| 质粒名称: | pGRG36 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 16666 |
| 存储实验室: | Nancy Craig |
| 载体骨架基本信息 | |
| 载体名称: | pGRG36 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 12549 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mTn7::MCS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | DQ460223 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SmaI, AvrII, NotI, PacI, and XhoI(未破坏) |
| 3' 克隆位点: | SmaI, AvrII, NotI, PacI, and XhoI(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGRG36 was a gift from Nancy Craig (Addgene plasmid # 16666 ; http://n2t.net/addgene:16666 ; RRID:Addgene_16666) |
| 参考文献部分: | Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. McKenzie GJ, Craig NL. BMC Microbiol. 2006 . 6():39. 10.1186/1471-2180-6-39 PubMed 16646962 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGRG36 12549 bp ds-DNA circular SYN 07-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGRG36
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12549)
AUTHORS McKenzie GJ, Craig NL
TITLE Fast, easy and efficient: site-specific insertion of transgenes into
enterobacterial chromosomes using Tn7 without need for selection of
the insertion event.
JOURNAL BMC Microbiol. 2006 . 6():39.
PUBMED 16646962
REFERENCE 2 (bases 1 to 12549)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 7, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12549
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(223..1101)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1128..1412
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 1335..1354
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
primer_bind complement(8103..8120)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 8273..8359
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 8451..8478
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 8497..8588
/gene="bla"
/label=AmpR promoter
CDS 8589..9449
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8807..8826)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind complement(9578..9597)
/label=F1ori-R
/note="F1 origin, reverse primer"
oriT 9620..9729
/note="incP origin of transfer"
primer_bind complement(9767..9786)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
rep_origin 10469..10691
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS 10739..11689
/codon_start=1
/gene="rep101 encoding an A56V mutation"
/product="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
/label=Rep101(Ts)
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
mobile_element 12122..12287
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
1 attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac
61 tttattgtcc gcccacaggc cacgatgcgt ccggcgtaga ggatctgctc atgtttgaca
121 gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
181 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
241 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
301 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
361 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
421 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
481 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
541 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
601 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
661 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
721 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
781 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
841 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
901 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
961 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
1021 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
1081 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
1141 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa
1201 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
1261 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
1321 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
1381 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta gcgaattcga
1441 gctcggtacc caagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
1501 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
1561 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc
1621 catggctaac agtacaagaa gttccttctt caggtcgttc ccaccgtatt tattctcata
1681 agacgggacg agtccatcat ttgctatctg acttagagct tgctgttttt ctcagtcttg
1741 agtgggagag cagcgtgcta gatatacgcg agcagttccc cttattacct agtgatacca
1801 ggcagattgc aatagatagt ggtattaagc atcctgttat tcgtggtgta gatcaggtta
1861 tgtctactga ttttttagtg gactgcaaag atggtccttt tgagcagttt gctattcaag
1921 tcaaacctgc agcagcctta caagacgagc gtaccttaga aaaactagaa ctagagcgtc
1981 gctattggca gcaaaagcaa attccttggt tcatttttac tgataaagaa ataaatcccg
2041 tagtaaaaga aaatattgaa tggctttatt cagtgaaaac agaagaagtt tctgcggagc
2101 ttttagcaca actatcccca ttggcccata tcctgcaaga aaaaggagat gaaaacatta
2161 tcaatgtctg taagcaggtt gatattgctt atgatttgga gttaggcaaa acattgagtg
2221 agatacgagc cttaaccgca aatggtttta ttaagttcaa tatttataag tctttcaggg
2281 caaataagtg tgcagatctc tgtattagcc aagtagtgaa tatggaggag ttgcgctatg
2341 tggcaaatta atgaggttgt gctatttgat aatgatccgt atcgcatttt ggctatagag
2401 gatggccaag ttgtctggat gcaaataagc gctgataaag gagttccaca agctagggct
2461 gagttgttgc taatgcagta tttagatgaa ggccgcttag ttagaactga tgacccttat
2521 gtacatcttg atttagaaga gccgtctgta gattctgtca gcttccagaa gcgcgaggag
2581 gattatcgaa aaattcttcc tattattaat agtaaggatc gtttcgaccc taaagtcaga
2641 agcgaactcg ttgagcatgt ggtccaagaa cataaggtta ctaaggctac agtttataag
2701 ttgttacgcc gttactggca gcgtggtcaa acgcctaatg cattaattcc tgactacaaa
2761 aacagcggtg caccagggga aagacgttca gcgacaggaa cagcaaagat tggccgagcc
2821 agagaatatg gtaagggtga aggaaccaag gtaacgcccg agattgaacg cctttttagg
2881 ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa agaccaccgt tgcctataga
2941 cgatttgtgg acttgtttgc tcagtatttt cctcgcattc cccaagagga ttacccaaca
3001 ctacgtcagt ttcgttattt ttatgatcga gaatacccta aagctcagcg cttaaagtct
3061 agagttaaag caggggtata taaaaaagac gtacgaccct taagtagtac agccacttct
3121 caggcgttag gccctgggag tcgttatgag attgatgcca cgattgctga tatttattta
3181 gtggatcatc atgatcgcca aaaaatcata ggaagaccaa cgctttacat tgtgattgat
3241 gtgtttagtc ggatgatcac gggcttttat atcggctttg aaaatccgtc ttatgtggtg
3301 gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg ccatttgtgc ccagcatgat
3361 attgagatta gtagctcaga ctggccgtgt gtaggtttgc cagatgtgtt gctagcggac
3421 cgtggcgaat taatgagtca tcaggtcgaa gccttagttt ctagttttaa tgtgcgagtg
3481 gaaagtgctc cacctagacg tggcgatgct aaaggcatag tggaaagcac ttttagaaca
3541 ctacaagccg agtttaagtc ctttgcacct ggcattgtag agggcagtcg gatcaaaagc
3601 catggtgaaa cagactatag gttagatgca tctctgtcgg tatttgagtt cacacaaatt
3661 attttgcgta cgatcttatt cagaaataac catctggtga tggataaata cgatcgagat
3721 gctgattttc ctacagattt accgtctatt cctgtccagc tatggcaatg gggtatgcag
3781 catcgtacag gtagtttaag ggctgtggag caagagcagt tgcgagtagc gttactgcct
3841 cgccgaaagg tctctatttc ttcatttggc gttaatttgt ggggtttgta ttactcgggg
3901 tcagagattc tgcgtgaggg ttggttgcag cggagcactg atatagctag acctcaacat
3961 ttagaagcgg cttatgaccc agtgctggtt gatacgattt atttgtttcc gcaagttggc
4021 agccgtgtat tttggcgctg taatctgacg gaacgtagtc ggcagtttaa aggtctctca
4081 ttttgggagg tttgggatat acaagcacaa gaaaaacaca ataaagccaa tgcgaagcag
4141 gatgagttaa ctaaacgcag ggagcttgag gcgtttattc agcaaaccat tcagaaagcg
4201 aataagttaa cgcccagtac tactgagccc aaatcaacac gcattaagca gattaaaact
4261 aataaaaaag aagccgtgac ctcggagcgt aaaaaacgtg cggagcattt gaagccaagc
4321 tcttcaggtg atgaggctaa agttattcct ttcaacgcag tggaagcgga tgatcaagaa
4381 gattacagcc tacccacata cgtgcctgaa ttatttcagg atccaccaga aaaggatgag
4441 tcatgagtgc tacccggatt caagcagttt atcgtgatac gggggtagag gcttatcgtg
4501 ataatccttt tatcgaggcc ttaccaccat tacaagagtc agtgaatagt gctgcatcac
4561 tgaaatcctc tttacagctt acttcctctg acttgcaaaa gtcccgtgtt atcagagctc
4621 ataccatttg tcgtattcca gatgactatt ttcagccatt aggtacgcat ttgctactaa
4681 gtgagcgtat ttcggtcatg attcgaggtg gctacgtagg cagaaatcct aaaacaggag
4741 atttacaaaa gcatttacaa aatggttatg agcgtgttca aacgggagag ttggagacat
4801 ttcgctttga ggaggcacga tctacggcac aaagcttatt gttaattggt tgttctggta
4861 gtgggaagac gacctctctt catcgtattc tagccacgta tcctcaggtg atttaccatc
4921 gtgaactcaa tgtagagcag gtggtgtatt tgaaaataga ctgctcgcat aatggttcgc
4981 taaaagaaat ctgcttgaat tttttcagag cgttggatcg agccttgggc tcgaactatg
5041 agcgtcgtta tggcttaaaa cgtcatggta tagaaaccat gttggctttg atgtcgcaaa
5101 tagccaatgc acatgcttta gggttgttgg ttattgatga aattcagcat ttaagccgct
5161 ctcgttcggg tggatctcaa gagatgctga acttttttgt gacgatggtg aatattattg
5221 gcgtaccagt gatgttgatt ggtaccccta aagcacgaga gatttttgag gctgatttgc
5281 ggtctgcacg tagaggggca gggtttggag ctatattctg ggatcctata caacaaacgc
5341 aacgtggaaa gcccaatcaa gagtggatcg cttttacgga taatctttgg caattacagc
5401 ttttacaacg caaagatgcg ctgttatcgg atgaggtccg tgatgtgtgg tatgagctaa
5461 gccaaggagt gatggacatt gtagtaaaac tttttgtact cgctcagctc cgtgcgctag
5521 ctttaggcaa tgagcgtatt accgctggtt tattgcggca agtgtatcaa gatgagttaa
5581 agcctgtgca ccccatgcta gaggcattac gctcgggtat cccagaacgc attgctcgtt
5641 attctgatct agtcgttccc gagattgata aacggttaat ccaacttcag ctagatatcg
5701 cagcgataca agaacaaaca ccagaagaaa aagcccttca agagttagat accgaagatc
5761 agcgtcattt atatctgatg ctgaaagagg attacgattc aagcctgtta attcccacta
5821 ttaaaaaagc gtttagccag aatccaacga tgacaagaca aaagttactg cctcttgttt
5881 tgcagtggtt gatggaaggc gaaacggtag tgtcagaact agaaaagccc tccaagagta
5941 aaaaggtttc ggctataaag gtagtcaagc ccagcgactg ggatagcttg cctgatacgg
6001 atttacgtta tatctattca caacgccaac ctgaaaaaac catgcatgaa cggttaaaag
6061 ggaaaggggt aatagtggat atggcgagct tatttaaaca agcaggttag ccatgagaaa
6121 ctttcctgtt ccgtactcga atgagctgat ttatagcact attgcacggg caggcgttta
6181 tcaagggatt gttagtccta agcagctgtt ggatgaggtg tatggcaacc gcaaggtggt
6241 cgctacctta ggtctgccct cgcatttagg tgtgatagca agacatctac atcaaacagg
6301 acgttacgct gttcagcagc ttatttatga gcatacctta ttccctttat atgctccgtt
6361 tgtaggcaag gagcgccgag acgaagctat tcggttaatg gagtaccaag cgcaaggtgc
6421 ggtgcattta atgctaggag tcgctgcttc tagagttaag agcgataacc gctttagata
6481 ctgccctgat tgcgttgctc ttcagctaaa taggtatggg gaagcctttt ggcaacgaga
6541 ttggtatttg cccgctttgc catattgtcc aaaacacggt gctttagtct tctttgatag
6601 agctgtagat gatcaccgac atcaattttg ggctttgggt catactgagc tgctttcaga
6661 ctaccccaaa gactccctat ctcaattaac agcactagct gcttatatag cccctctgtt
6721 agatgctcca cgagcgcaag agctttcccc aagccttgag cagtggacgc tgttttatca
6781 gcgcttagcg caggatctag ggctaaccaa aagcaagcac attcgtcatg acttggtggc
6841 ggagagagtg aggcagactt ttagtgatga ggcactagag aaactggatt taaagttggc
6901 agagaacaag gacacgtgtt ggctgaaaag tatattccgt aagcatagaa aagcctttag
6961 ttatttacag catagtattg tgtggcaagc cttattgcca aaactaacgg ttatagaagc
7021 gctacagcag gcaagtgctc ttactgagca ctctataacg acaagacctg ttagccagtc
7081 tgtgcaacct aactctgaag atttatctgt taagcataaa gactggcagc aactagtgca
7141 taaataccaa ggaattaagg cggcaagaca gtctttagag ggtggggtgc tatacgcttg
7201 gctttaccga catgacaggg attggctagt tcactggaat caacagcatc aacaagagcg
7261 tctggcaccc gcccctagag ttgattggaa ccaaagagat cgaattgctg tacgacaact
7321 attaagaatc ataaagcgtc tagatagtag ccttgatcac ccaagagcga catcgagctg
7381 gctgttaaag caaactccta acggaacctc tcttgcaaaa aatctacaga aactgccttt
7441 ggtagcgctt tgcttaaagc gttactcaga gagtgtggaa gattatcaaa ttagacggat
7501 tagccaagct tttattaagc ttaaacagga agatgttgag cttaggcgct ggcgattatt
7561 aagaagtgca acgttatcta aagagcggat aactgaggaa gcacaaagat tcttggaaat
7621 ggtttatggg gaagagtgag tggttaggct agctacattt aatgacaatg tgcaggttgt
7681 acatattggt catttattcc gtaactcggg tcataaggag tggcgtattt ttgtttggtt
7741 taatccaatg caagaacgga aatggactcg atttactcat ttgcctttat taagtcgagc
7801 taaggtggtt aacagtacaa caaagcaaat aaataaggcg gatcgtgtga ttgagtttga
7861 agcatcggat cttcaacgag ccaaaataat cgattttcct aatctctcgt cctttgcttc
7921 cgtacgcaac aaggatggag cgcagagttc atttatttac gaagctgaaa caccatatag
7981 caagactcgt tatcacatcc cacagttaga gctagctcgg tcattatttt taggggatcc
8041 tctagagtcg acctgcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc
8101 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
8161 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
8221 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
8281 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
8341 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
8401 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
8461 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
8521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
8581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
8641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
8701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
8761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
8821 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
8881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
8941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
9001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
9061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
9121 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
9181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
9241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
9301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
9361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
9421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
9481 agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
9541 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
9601 tttctcgcca cgttcgccgc cggccagcct cgcagagcag gattcccgtt gagcaccgcc
9661 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
9721 atcctgcccg gcggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
9781 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
9841 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
9901 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
9961 gagtcgcata agggagagcg tcgatccccg acagtaagac gggtaagcct gttgatgata
10021 ccgctgcctt actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt
10081 ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca
10141 catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg
10201 ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact
10261 gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg
10321 atggtcggag acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa
10381 gcctttaggg ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt
10441 gtaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt
10501 tatctttttt tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa
10561 aacacacaaa ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc
10621 aaaggtctag cagaatttac agatacccac aactcaaagg aaaaggacta gtaattatca
10681 ttgactagcc catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat
10741 gtctgaatta gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga
10801 gcatgaaacc aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc
10861 tacaaggaaa gaacggacgg tatcgttcac ttataaccaa tacgttcaga tgatgaacat
10921 cagtagggaa aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac
10981 tgtggaaatc aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc
11041 caagttctca agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt
11101 ccagttaaaa aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata
11161 ctctatgagg atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc
11221 aaatatagag attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca
11281 tgagtttaaa aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta
11341 cagcaatatg aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca
11401 agttgaacta gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat
11461 gaatggtgac aaaataccaa caaccattac atcagattcc tacctacata acggactaag
11521 aaaaacacta cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt
11581 tttgagtgac atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa
11641 acaacgaacc acactagaga acatactggc taaatacgga aggatctgag gttcttatgg
11701 ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc
11761 gttacatatc aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag
11821 atacatcaga gcttttacga gtttttggtg catttaaagc tgttcaccat gaacagatcg
11881 acaatgtaac agatgaacag catgtaacac ctaatagaac aggtgaaacc agtaaaacaa
11941 agcaactaga acatgaaatt gaacacctga gacaacttgt tacagctcaa cagtcacaca
12001 tagacagcct gaaacaggcg atgctgctta tcgaatcaaa gctgccgaca acacgggagc
12061 cagtgacgcc tcccgtgggg aaaaaatcat ggcaattctg gaagaaatag cgctttcagc
12121 ctgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta
12181 tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa
12241 attaaaaatg acaaaatagt ttggaactag atttcactta tctggttggt cgacactagt
12301 attaccctgt tatccctaga tttaaatgat atcggatcct agtaagccac gttttaatta
12361 atcagatccc gggtcaatag cggccgccac tcgagcacct aggaggcgcg ccacggccgc
12421 gtcgacccca cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt
12481 tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag
12541 tttacaact
//
