pLVX-Het-Nuc1

价格:4000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pLVX-Het-Nuc1
质粒类型: 慢病毒载体;双顺反子载体;亚细胞定位载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8923 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: DmrA
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-Het-Nuc1组成型表达DmrA融合蛋白;利用信号受体的二聚化作用研究细胞信号通路;DmrA含有核定位信号。
产品目录号: 635075
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-Het-Nuc1载体图谱



pLVX-Het-Nuc1 多克隆位点

pLVX-Het-Nuc1 载体特征

载体简介

载体序列

LOCUS       pLVX-Het-Nuc1   8923 bp    DNA   circular             8-NOV-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No.635074, 635075
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=CTS/cppt\(DNA\flap)
     promoter        2185..2788
                     /label=pCMV
     misc_feature    4845..5436
                     /label=WPRE
     rep_origin      complement(6746..7416)
                     /label=pUC\origin
     CDS             complement(7561..8557)
                      /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             5640..6276
                     /label=3'\LTR
     CDS             3797..4831
                     /label=Hyg(R)
     misc_feature    3198..3773
                     /label=IRES
     misc_feature    2822..2845
                     /label=NLS
     misc_feature    2846..3172
                     /label=DmrA
BASE COUNT     2304 a      2157 c      2307 g      2155 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 
     2821 tatgcctaag aagaagagaa aggtgtctag aggagtgcag gtggaaacca tctccccagg 
     2881 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct 
     2941 tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct 
     3001 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca 
     3061 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat 
     3121 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaagcggccg 
     3181 cggatcctac gtatgaccgc ccctctccct cccccccccc taacgttact ggccgaagcc 
     3241 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 
     3301 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc 
     3361 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc 
     3421 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc 
     3481 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg 
     3541 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 
     3601 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat 
     3661 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc 
     3721 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc 
     3781 acaacccgta ccaaagatgg atagatccgg aaagcctgaa ctcaccgcga cgtctgtcga 
     3841 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga 
     3901 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag 
     3961 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct 
     4021 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc 
     4081 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct 
     4141 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg 
     4201 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg 
     4261 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc 
     4321 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg 
     4381 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac 
     4441 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat 
     4501 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag 
     4561 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga 
     4621 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg 
     4681 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag 
     4741 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg 
     4801 ccccagcact cgtccgaggg caaaggaata gacgcgtctg gaacaatcaa cctctggatt 
     4861 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 
     4921 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 
     4981 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 
     5041 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 
     5101 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 
     5161 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 
     5221 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 
     5281 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 
     5341 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 
     5401 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct 
     5461 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg 
     5521 gctagaagca caagaggagg aggaggtggg tttttccagt cacacctcag gtacctttaa 
     5581 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac 
     5641 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca 
     5701 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
     5761 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
     5821 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
     5881 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
     5941 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
     6001 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
     6061 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
     6121 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     6181 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     6241 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 
     6301 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag 
     6361 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 
     6421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     6481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     6541 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 
     6601 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     6661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     6721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     6781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     6841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     6901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     6961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     7021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     7081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     7141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     7201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 
     7261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     7321 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     7381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     7441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     7501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     7681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     8521 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt 
     8581 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     8641 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     8701 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc 
     8761 ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa 
     8821 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt 
     8881 aaagaaattg tatttgttaa atatgtacta caaacttagt agt 
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng