pCMV-SPORT2

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pCMV-SPORT2
质粒类型: 哺乳动物表达载体;cDNA克隆载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 4774 bp
5' 测序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: DH5α 等
宿主细胞(系): 哺乳动物细胞
备注: 用于cDNA文库的构建和表达
产品目录号: --
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-SPORT2载体图谱
pCMV-SPORT2多克隆位点

载体简介

载体序列

LOCUS       pCMV-Sport2	4474 bp 	DNA	SYN
DEFINITION  pCMV-Sport2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="pCMV-Sport2"
                     /mol_type="other DNA"
     terminator      complement(135..254)
                     /label="SV40_PA_terminator"
     misc_feature    complement(149..168)
                     /label="EBV_rev_primer"
     misc_feature    complement(542..557)
                     /label="SV40_int"
     misc_feature    649..671
                     /label="M13_pUC_fwd_primer"
     promoter        664..680
                     /label="M13_forward20_primer"
     promoter        691..709
                     /label="T7_promoter"
     misc_feature    complement(833..850)
                     /label="Sp6_primer"
     misc_feature    complement(886..908)
                     /label="M13_pUC_rev_primer"
     promoter        complement(932..1051)
                     /label="CMV2_promoter"
     misc_feature    complement(994..1018)
                     /label="LNCX_primer"
     promoter        complement(994..1065)
                     /label="CMV_promoter"
     misc_feature    complement(1001..1020)
                     /label="pCEP_fwd_primer"
     promoter        complement(1017..1521)
                     /label="CMV_immearly_promoter"
     misc_feature    complement(1044..1064)
                     /label="CMV_fwd_primer"
     misc_feature    complement(1230..1517)
                     /label="CAG_enhancer"
     misc_feature    complement(1541..1563)
                     /label="M13_pUC_rev_primer"
     promoter        1754..1782
                     /label="AmpR_promoter"
     rep_origin      complement(2054..2673)
                     /label="pBR322_origin"
     misc_feature    2735..2768
                     /label="loxP"
     gene            complement(2870..3730)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2870..3730)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      complement(4150..4456)
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
   61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
  121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
  181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
  241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
  301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
  361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
  421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
  481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
  541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
  601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagatctcc cagtcacgac
  661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg agagagctat
  721 gacgtcgcat gcacgcgtaa gcttgggccc ctcgagggat cctctagagc ggccgccgac
  781 tagtgagctc gtcgacccgg gaattccgga ccggtacctg caggcgtacc ttctatagtg
  841 tcacctaaat agctttttgc aaaagcctag tggtcatagc tgtttcctgt gtgaaattgt
  901 tatccgctcc gcggcctagg ctagagtccg gaggctggat cggtcccggt gtcttctatg
  961 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct
 1021 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
 1081 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
 1141 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
 1201 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
 1261 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
 1321 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
 1381 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
 1441 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
 1501 atttaccgta agttatgtaa cgacctgcac gatgctgttt cctgtgtgaa attgttatcc
 1561 gctcacaatt ccacacatta tacgagccgg aagctataaa gtgtaaagcc tggggtgcct
 1621 aatgagtgaa agggcctcgt atacgcctat ttttataggt taatgtcatg ataataatgg
 1681 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
 1741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
 1801 aataatattg aaaaacgcgc gaattgcaag ctctgcatta atgaatcggc caacgcgcgg
 1861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
 1921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
 1981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
 2041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
 2101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
 2161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
 2221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
 2281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
 2341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
 2401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
 2461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
 2521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
 2581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
 2641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
 2701 acgaaaactc acgttaaggg attttggtca tgccataact tcgtatagca tacattatac
 2761 gaagttatgg catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
 2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
 2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
 2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
 3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
 3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
 3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
 3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
 3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
 3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
 3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
 3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
 3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
 3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
 3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
 3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
 3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
 3781 tgccaggggt gggcacacat atttgatacc agcgatccct acacagcaca taattcaatg
 3841 cgacttccct ctatcgcaca tcttagacct ttattctccc tccagcacac atcgaagctg
 3901 ccgagcaagc cgttctcacc agtccaagac ctggcatgag cggatacata tttgaatgta
 3961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
 4021 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
 4081 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
 4141 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
 4201 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
 4261 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
 4321 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
 4381 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
 4441 ccgccgcgct taatgcgccg ctacagggcg cgtc
//

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