| 质粒名称: | pRSF-pLtetO-gfp |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 89476 |
| 存储实验室: | George Church |
|
载体骨架基本信息 |
|
| 载体名称: | pRSF |
| 载体出品公司: | Sergey V Mashko |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8728 bp |
| 完整质粒大小: | 9664 bp |
| 载体修饰: | 插入 pLtetO-gfpmut3b |
| 载体类型: | -- |
| 筛选标记: | 卡巴尼西林 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pLtetO-GFPmut3b |
| 物种来源: | -- |
| 插入大小: | 717 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | pLtetO |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | GGCATCAAATTAAGCAGAAGGCCATCCTGA |
| 3' 测序引物 | GCTTCCCGTTTCAGCTGCGTTTC |
|
文献引用方法 |
|
| 材料和方法部分: | pRSF-pLtetO-gfp was a gift from George Church (Addgene plasmid # 89476 ; http://n2t.net/addgene:89476 ; RRID:Addgene_89476) |
| 参考文献部分: | Vibrio natriegens, a new genomic powerhouse. Lee HH, Ostrov N, Wong BG, Gold MA, Khalil A, Church GM. bioRxiv 058487 10.1101/058487 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRSF-pLtetO-gfp 9716 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Broad-host plasmid expressing gfp under pLtetO promoter.
ACCESSION .
VERSION .
KEYWORDS pRSF-pLtetO-gfp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9716)
AUTHORS Lee HH, Ostrov N, Wong BG, Gold MA, Khalil A, Church GM
TITLE Vibrio natriegens, a new genomic powerhouse
JOURNAL bioRxiv 058487
REFERENCE 2 (bases 1 to 9716)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9716
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..74
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
protein_bind 1..19
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 26..44
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
RBS 84..95
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 102..818
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/label=GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(132..160)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 755..775
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
terminator 878..924
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin 1607..2001
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010; requires the RSF1010 RepA/B/C proteins for
replication (Scholz et al., 1989)"
oriT 2343..2429
/label=RSF1010 oriT
/note="origin of transfer of the broad-host-range plasmid
RSF1010 (Scholz et al., 1989)"
CDS 3668..4639
/codon_start=1
/gene="repB"
/product="replication protein B of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepB
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEETGKAMAEASPALAERKPGHEAD
YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
CDS 5150..5989
/codon_start=1
/gene="repA"
/product="replication protein A of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepA
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
promoter 7093..7123
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind 7131..7147
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7136..7158
/label=M13/pUC Reverse
/note="In lacZ gene"
RBS 7172..7194
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 7202..8284
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind complement(7221..7240)
/label=LacI-R
/note="LacI, reverse primer"
CDS 8418..9278
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
terminator 9477..9562
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 9654..9681
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
ORIGIN
1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc
61 aggacacact gaccgaattc attaaagagg agaaaggtac catgagtaaa ggagaagaac
121 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
181 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
241 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
301 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
361 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
421 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
481 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
541 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
601 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
661 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
721 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
781 ctgggattac acatggcatg gatgaactat acaaataaaa gcttgatatc gaattcctgc
841 agcccggggg atcccatggt acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc
901 gaaagactgg gcctttcgtt ttatgcggcc gcgatcaagc aggtgcgaca gacgtcatac
961 tagatatcaa gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg
1021 ctggccaaag ccttagcgta ggattccgcc ccttcccgca aacgacccca aacaggaaac
1081 gcagctgaaa cgggaagctc aacacccact gacgcatggg ttgttcaggc agtacttcat
1141 caaccagcaa ggcggcactt tcggccatcc gccgcgcccc acagctcggg cagaaaccgc
1201 gacgcttaca gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta
1261 gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc
1321 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
1381 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat
1441 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt
1501 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt
1561 gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat
1621 aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca
1681 aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt
1741 atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg
1801 cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc
1861 gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac
1921 cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag
1981 ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca
2041 tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca
2101 tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc
2161 atggccccca ccagcaccct gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt
2221 gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc
2281 cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc
2341 tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggg
2401 cacttaccgg tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct
2461 ccgagggcca ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta
2521 tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga
2581 ctacatccag cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc
2641 cgggcacatg ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta
2701 tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac
2761 cctcgaccag cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg
2821 cctgccgtat acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat
2881 gatctccgag cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta
2941 caacggcaag accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc
3001 atggcttgag cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc
3061 tggccacgac gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc
3121 cggtgttcac ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg
3181 ggcagacgtg gccctgaaca tcgacaccgc caacacccag atcatcgact tacaggaata
3241 ccgggaggca atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg
3301 agttagcgga gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc
3361 ggcaggtcat gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag
3421 cccagcgcca gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg
3481 ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc
3541 tatggctaac cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt
3601 tgctcttgct cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg
3661 tggcccgatg aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg
3721 ctgtgagcgc ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga
3781 atggtcagcc gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg
3841 caatgacgtg tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga
3901 cctcagcgag tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt
3961 ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga
4021 acttcggggg cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc
4081 cgacagccgc cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac
4141 ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc
4201 tggcccggcg ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga
4261 gaaggcccgc aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg
4321 cacggcgctg gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga
4381 cctcagcaag tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga
4441 ggaaaccggc aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca
4501 cgaagcggat tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct
4561 tgcgcgggcc gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg
4621 gccagatttc agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc
4681 acgcacagaa gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat
4741 caaaagtgac aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga
4801 ggctggccgc ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc
4861 cttgttccgc gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag
4921 accaaaagga caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc
4981 gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg
5041 acgaatacga ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta
5101 gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca
5161 taagcctatc aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt
5221 gcccaacatg gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc
5281 catgctggcc ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg
5341 cgaactgccc accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca
5401 tcaccgcctg cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga
5461 cggcctgctg atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt
5521 cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg
5581 gttccacatc gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga
5641 ggccatcgcc gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc
5701 ggccatgatg ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga
5761 taacatccgc tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg
5821 tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg
5881 cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt
5941 gctggagagg cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca
6001 gcctcagcca cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc
6061 tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggtgacggca
6121 agcggatcga gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag
6181 ggctggtggc catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag
6241 gcggacggca gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgatgcca
6301 tggtggtcaa aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg
6361 gtggggcgct caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg
6421 cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg
6481 cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg
6541 gccagcatgt gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc
6601 tgctgcacca gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag
6661 ataccttgtg cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc
6721 gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt
6781 tcgcggcggg caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct
6841 attgtaaaca agacattttt atcttttata ttcaatggct tattttcctg ctaattggta
6901 ataccatgaa aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact
6961 gagcgctgcc gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct
7021 tctgctccga tctgcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta
7081 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggaa ttgtgagcgg
7141 ataacaattt cacacaggat ctagaaataa ttttgtttaa ctttaagaag gagatataca
7201 tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
7261 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
7321 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
7381 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
7441 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
7501 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
7561 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
7621 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
7681 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
7741 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
7801 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
7861 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
7921 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
7981 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
8041 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
8101 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
8161 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
8221 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
8281 gtgagcggat ctcgaagctg atccgcggcc gccacgttgt gtctcaaaat ctctaatgtt
8341 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
8401 gtaatacaag gggtgttatg agcattcagc attttcgtgt ggcgctgatt ccgttttttg
8461 cggcgttttg cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg
8521 aagatcaact gggtgcgcgc gtgggctata ttgaactgga tctgaacagc ggcaaaattc
8581 tggaatcttt tcgtccggaa gaacgttttc cgatgatgag cacctttaaa gtgctgctgt
8641 gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca actgggccgt cgtattcatt
8701 atagccagaa cgatctggtg gaatatagcc cggtgaccga aaaacatctg accgatggca
8761 tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag cgataacacc gcggcgaacc
8821 tgctgctgac gaccattggc ggtccgaaag aactgaccgc gtttctgcat aacatgggcg
8881 atcatgtgac ccgtctggat cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg
8941 aacgtgatac caccatgccg gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg
9001 agctgctgac cctggcaagc cgccagcaac tgattgattg gatggaagcg gataaagtgg
9061 cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt tattgcggat aaaagcggtg
9121 cgggcgaacg tggcagccgt ggcattattg cggcgctggg cccggatggt aaaccgagcc
9181 gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt aaccgtcaga
9241 ttgcggaaat tggcgcgagc ctgattaaac attggtaatc agaattggtt aattggttga
9301 ttaccctgtt atccctatct agagtcgacc tgcaggcatg caagcttaga tcctttgcct
9361 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
9421 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcattaaat
9481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
9541 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
9601 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
9661 catcctgacg gatggccttt ttgcgtttct acaaactctt tttgggtacc agatct
//
