LOCUS pCMV-SynA 6020 bp ds-DNA circular SYN 14-SEP-2021
DEFINITION Expresses mouse syncytin A in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-SynA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6020)
AUTHORS Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
RK, Koonin EV, Zhang F
TITLE Mammalian retrovirus-like protein PEG10 packages its own mRNA and
can be pseudotyped for mRNA delivery.
JOURNAL Science. 2021 Aug 20;373(6557):882-889. doi:
10.1126/science.abg6155.
PUBMED 34413232
REFERENCE 2 (bases 1 to 6020)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 14, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6020
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 201..225
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 338..813
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
regulatory 881..890
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 2822..3216
/label=-beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
primer_bind complement(3555..3572)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3726..4314)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3806..3825)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4485..5345)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5108..5127
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5346..5450)
/gene="bla"
/label=AmpR promoter
primer_bind complement(5520..5539)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
enhancer 5641..6020
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
301 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
361 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
421 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
481 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
541 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
601 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
661 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
721 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
781 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
841 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgg ttcgtccttg
901 ggttttctgt ctcctcctgt tcccttgttc ctctgcctac tcggacagct ggatgcccct
961 ggtaaacctc actcaacacc tcctccagga ggctaactct tccttctctt ccaactgctg
1021 ggtctgctta tccatccaaa cccagcgctc tctagccatg ccagccccac taaggacttg
1081 gacagagaca cccatgaaac ttcgaatcat gtactcagcc cggaccctct ccggcccata
1141 ccctatcacc gaccttgaga ggcgcctcca gaatttccaa ccattgactc cccactcctc
1201 ttttgtcaac cctgaccagc gggccattgc tttccttcag atcaccagcg tgacaggcat
1261 acttcccata ctttctcgga tcacctcggt gagatacccc gatgaccacg tctatgaatc
1321 tgcccagcgc cccatatggg gctcactctc cacccagacg atcctcacct cccaggcccc
1381 tctctgcata tcccgcttct tcaagaattc aaaccatgcc accttcgtgg gcaaactccc
1441 tgcctctctt tgcaatcaca cctttcagct ctccccctct gccaaccacc aatccataga
1501 tctgtcctcc agctatgcat tcgccccatt aatggccatg ccagggtcta aatggagaaa
1561 ccccttacgc ttttcaggac ccccttccct gaactcaggg atgcctcact actcctgccc
1621 gatagatgac atccactgcc acacctaccc caccaccccc tggaggtcct gtccttcctt
1681 cccagctagc acctgctata atctcaccct attcgagccg gacaattcga gccaccctat
1741 taccctgtct gtggacacca catacttcaa gattaaactc cagggacaca aagaccccta
1801 tccactcttt cagtaccagc ccctcatggg ggcagccctc tctggacaat attcaatctg
1861 ggaatatgaa cccactgtta agaaaaacgg cggtatcact ccaaatatct tctcccacct
1921 tgtctcctta acatactcct tctgcctcaa ctcctctggt gttttcttcc tctgtggaaa
1981 ctcaacttat gtctgtctcc cggccaattg gtccggcgtc tgtacccttg tcttccaata
2041 cccggatatt gaactccttc ccaacaacca aaccatatct gtcccgcttt ttgctacagt
2101 tccctcctct gtccccgctt ctcgccggaa gcgagccctt cctctccttc ctctcctcgc
2161 gggcctgggc attgcttctg ccctggggtt aggcatcgcg ggtatcacca cctcaactgt
2221 gtatttccaa cagctttcca aggctctctc ggacagccta gatgaaatag ccacctccat
2281 catcagcctc caagaccaaa tagactcgct ggcgggtgtc gttctccaaa accgcagagc
2341 tctggacctc attgtggctg agaggggggg cacctgcctc ttcctccagg aagagtgctg
2401 cttctacata aaccagtccg gggtagtccg gcacgcggca aggaaacttc gagaaagggc
2461 ctcggaactc ggcacaagct cgagttcttg gatccagtgg ctggggctag gaccctggct
2521 gccctcttgg ttgacttccc tcatggctcc cattctcttt atcctggtac tgctggtttt
2581 caggccttgt cttcttaact gcctgactca ttctgtatcg cggcgaatga gttctttcat
2641 tcacaccacc accgaaggac acgtggacaa gatccttctg cttcgagagt cccagtacaa
2701 gagacttccc caagagcccc cggaggagga tgccgtctag gaattcaccc caccagtgca
2761 ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta
2821 agctcgcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta
2881 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat
2941 ttattttcat tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg
3001 tgggaggtca gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa
3061 tacactatat cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg
3121 caacagcccc tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt
3181 gatttggagg ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc
3241 tcatgaatgt cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact
3301 cagttctctt gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc
3361 gagatggttt ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt
3421 ctacttgaag aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct
3481 gcctccccca ctcacagtga cccggaatcc ctcgacatgg cagtctagca ctagtgcggc
3541 cgcagatctg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
4021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
4081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
4141 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
4201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
4261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4861 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
5041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
5101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
5161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
5221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
5281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
5341 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5461 cgaaaagtgc cacctgacgt ggatcccctg agggggcccc catgggctag aggatccggc
5521 ctcggcctct gcataaataa aaaaaattag tcagccatga gcttggccca ttgcatacgt
5581 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
5641 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
5701 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
5761 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
5821 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
5881 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
5941 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
6001 tagtcatcgc tattaccatg
//
