| 质粒名称: | pBABe-HA-ER-IPpoI |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32565 |
| 存储实验室: | Michael Kastan |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-Puro |
| 载体出品公司: | margenstern and L (1991) PMID Z194165 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | I-PpoI |
| 物种来源: | Physarum polycephalum |
| 插入大小: | 1514 bp |
| 基因突变: | -- |
| GenBank编号 | PMD 17473858 |
| 启动子 | -- |
| 标签/融合蛋白: |
HA（插入件上的N端子）
ER（插入件上的N端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI（克隆过程中销毁） |
| 3' 克隆位点: | BamHI（克隆过程中销毁） |
| 5' 测序引物 | PBABE 5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBABe-HA-ER-IPpoI was a gift from Michael Kastan (Addgene plasmid # 32565 ; http://n2t.net/addgene:32565 ; RRID:Addgene_32565) |
| 参考文献部分: | Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair. Berkovich E, Monnat RJ Jr, Kastan MB. Nat Cell Biol. 2007 Jun;9(6):683-90. Epub 2007 May 7. 10.1038/ncb1599 PubMed 17486112 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABe-HA-ER-IPpoI 6600 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABe-HA-ER-IPpoI
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6600)
AUTHORS Berkovich E, Monnat RJ Jr, Kastan MB
TITLE Roles of ATM and NBS1 in chromatin structure modulation and DNA
double-strand break repair.
JOURNAL Nat Cell Biol. 2007 Jun;9(6):683-90. Epub 2007 May 7.
PUBMED 17486112
REFERENCE 2 (bases 1 to 6600)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6600
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 942..1358
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1288..1310
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1326..1342
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1386..1412
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(2930..2950)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2935..3264
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3115..3250
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3177..3196
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3274..3873
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3274..3293)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3770..3790
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 4094..4563
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(4693..4710)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4864..5452)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4944..4963)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5612..6472)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6235..6254
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6473..6577)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatcatc gaattcacca
1381 tggcataccc atacgatgtt ccagattacg ctagcggatc tcgaaatgaa atgggtgctt
1441 caggagacat gagggctgcc aacctttggc caagccctct tgtgattaag cacactaaga
1501 agaatagccc tgccttgtcc ttgacagctg accagatggt cagtgccttg ttggatgctg
1561 aaccgcccat gatctattct gaatatgatc cttctagacc cttcagtgaa gcctcaatga
1621 tgggcttatt gaccaaccta gcagataggg agctggttca tatgatcaac tgggcaaaga
1681 gagtgccagg ctttggggac ttgaatctcc atgatcaggt ccaccttctc gagtgtgcct
1741 ggctggagat tctgatgatt ggtctcgtct ggcgctccat ggaacacccg gggaagctcc
1801 tgtttgctcc taacttgctc ctggacagga atcaaggtaa atgtgtggaa ggcatggtgg
1861 agatctttga catgttgctt gctacgtcaa gtcggttccg catgatgaac ctgcagggtg
1921 aagagtttgt gtgcctcaaa tccatcattt tgcttaattc cggagtgtac acgtttctgt
1981 ccagcacctt gaagtctctg gaagagaagg accacatcca ccgtgtcctg gacaagatca
2041 cagacacttt gatccacctg atggccaaag ctggcctgac tctgcagcag cagcatcgcc
2101 gcctagctca gctccttctc attctttccc atatccggca tatgagtaac aaacgcatgg
2161 agcatctcta caacatgaaa tgcaagaacg tggtacccct ctatgacctg ctcctggaga
2221 tgttggatgc ccaccgcctt catgccccag ccagtcgcat gggagtgccc ccagaggagc
2281 ccagccagac ccagctggcc accaccagct ccacttcagc acattcctta caaacctact
2341 acataccccc ggaagcagag ggcttcccca acacgatcgg atctatggcg ctcaccaatg
2401 ctcaaatctt ggctgtgatt gacagctggg aagaaacagt cggtcagttt ccagtgataa
2461 cgcaccatgt accattaggt ggcggtctgc aaggaacgct ccattgttac gagatccccc
2521 tagcagctcc ttatggggtt ggctttgcta agaatgggcc tacccgctgg caatacaaac
2581 ggacaatcaa tcaagtcgtc cacagatggg gatcccacac agtccctttt ctattagaac
2641 cggataacat caacggcaaa acctgcacag catcgcacct atgtcataat actcgatgcc
2701 acaatccctt gcacttgtgc tgggagtcac tagacgacaa caaaggcaga aactggtgcc
2761 cgggtcccaa cgggggatgt gtccatgcgg tggtttgttt aaggcagggt ccgttgtacg
2821 gcccgggggc gactgtggca ggtcctcaac aaaggggcag tcactttgtg gtataaagat
2881 cccagtgtgg tggtacgtag gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt
2941 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
3001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
3061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
3121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
3181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
3241 tttggaggcc taggcttttg caaaaagctt accatgaccg agtacaagcc cacggtgcgc
3301 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
3361 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
3421 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
3481 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
3541 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
3601 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
3661 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
3721 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
3781 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
3841 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
3901 accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt tagtctccag
3961 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
4021 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
4081 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
4141 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
4201 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
4261 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
4321 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
4381 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
4441 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
4501 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
4561 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg
4621 gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
4681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
4741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
4801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
4861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
4921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
4981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
5041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
5101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
5161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
5221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
5281 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
5341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
5401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
5461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
5521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgc aaatcaatct
5581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
5641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
5701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
5761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
5821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
5881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
5941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
6001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
6061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
6121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
6181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
6241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
6301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
6361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
6421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
6481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
6541 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
