pLVX-rHom-Sec1

价格:4560元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-rHom-Sec1
质粒类型: 慢病毒载体;双顺反子载体;信号通路载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 9550 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢毒载体pLVX-rHom-Sec1载体表达DmrD融合蛋白;
利用信号受体的可逆二聚化作用研究细胞信号通路;
DmrD的N端带有信号肽。
产品目录号: 635077
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-rHom-Sec1载体图谱



pLVX-rHom-Sec1 多克隆位点

pLVX-rHom-Sec1 载体特征

载体简介

载体序列

LOCUS       pLVX-rHom-sec1   9550 bp    DNA   circular            10-NOV-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Catalog Nos. 635077, 635076
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=CTS/cppt\(DNA\flap)
     promoter        2185..2788
                     /label=pCMV
     misc_feature    5472..6063
                     /label=WPRE
     rep_origin      complement(7373..8043)
                     /label=pUC\origin
     CDS             complement(8188..9184)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             6267..6903
                     /label=3'\LTR
     misc_feature    4251..4826
                     /label=IRES
     CDS             4859..5458
                     /label=Puro(R)
     misc_feature    2822..2899
                     /label=Secretory\signal\sequence
     CDS             2903..3226
                     /label=DmrD
     CDS             3557..3880
                     /label=DmrD
     CDS             3230..3553
                     /label=DmrD
     CDS             3884..4207
                     /label=DmrD
BASE COUNT     2438 a      2396 c      2500 g      2216 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 
     2821 tatggctaca ggctcccgga cgtccctgct cctggctttt ggcctgctct gcctgccctg 
     2881 gcttcaagag ggcagtgcct ctagaggagt gcaggtggag actatctccc caggagacgg 
     2941 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga 
     3001 tggaaagaaa atggattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa 
     3061 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc 
     3121 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc 
     3181 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta gaggagtgca 
     3241 ggtggagact atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt 
     3301 ggtgcactac accgggatgc ttgaagatgg aaagaaaatg gattcctccc gggacagaaa 
     3361 caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt 
     3421 tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg 
     3481 tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct 
     3541 tctaaaactg gaaactagag gagtgcaggt ggagactatc tccccaggag acgggcgcac 
     3601 cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa 
     3661 gaaaatggat tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga 
     3721 ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact 
     3781 gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca 
     3841 tgccactctc gtcttcgatg tggagcttct aaaactggaa actagaggag tgcaggtgga 
     3901 gactatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca 
     3961 ctacaccggg atgcttgaag atggaaagaa aatggattcc tcccgggaca gaaacaagcc 
     4021 ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca 
     4081 gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac 
     4141 tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa 
     4201 actggaaact agtgctagaa accccagcgg ccgcggatcc tacgtatgac cgcccctctc 
     4261 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 
     4321 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 
     4381 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 
     4441 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 
     4501 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 
     4561 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 
     4621 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 
     4681 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 
     4741 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg 
     4801 ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac gaccttccat 
     4861 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 
     4921 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 
     4981 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 
     5041 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 
     5101 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 
     5161 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 
     5221 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 
     5281 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 
     5341 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 
     5401 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctagac 
     5461 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 
     5521 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 
     5581 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 
     5641 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 
     5701 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 
     5761 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 
     5821 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 
     5881 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 
     5941 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 
     6001 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 
     6061 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac 
     6121 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt 
     6181 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta 
     6241 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag 
     6301 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca 
     6361 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag 
     6421 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg 
     6481 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc 
     6541 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat 
     6601 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg 
     6661 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact 
     6721 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
     6781 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
     6841 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
     6901 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 
     6961 gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc 
     7021 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     7081 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 
     7141 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     7201 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     7261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     7321 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     7381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     7441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     7501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     7561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     7621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     7681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     7741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     7801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     7861 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     7921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg 
     7981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     8041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     8101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     8161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     8221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     8281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     8341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     8401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     8461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     8521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     8581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     8641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     8701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     8761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     8821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     8881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     8941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     9001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     9061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     9121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
     9181 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca 
     9241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     9301 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa 
     9361 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat 
     9421 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa 
     9481 atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa 
     9541 acttagtagt 
//

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