pMSCVpuro

价格:1200元

联系方式:I47-825O-882O

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pMSCV-puro载体基本信息

出品公司: Clontech
载体名称: pMSCVpuro, pMSCV-puro, pMSCV puro
质粒类型: 哺乳动物细胞逆病毒基因表达载体
高拷贝/低拷贝: 低拷贝
启动子: LTR
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6264bp
5' 测序引物及序列: MSCV-F: CCCTTGAACCTCCTCGTTCGACC
3' 测序引物及序列: pMSCV-R: GAGACGTGCTACTTCCATTTGTC
载体标签: --
载体抗性: 氨苄
筛选标记: 潮霉素(Hygromycin)
备注: 逆病毒表达载体,优化后,有利于在造血干细胞中和癌细胞中进行基因表达。
产品目录号: 634401
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

pMSCV-puro载体质粒图谱和多克隆位点信息

pMSCVpuro载体图谱



pMSCVpuro多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

pMSCV-puro载体简介

pMSCV-puro载体序列

LOCUS       pMSCVpuro   6264 bp    DNA   circular             4-DEC-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 634401,631461
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..515
                     /label=5'\MMSV\LTR
     CDS             complement(4473..5330)
                     /label=Amp(R)
     rep_origin      3710..3710
                     /label=pUC\origin
     misc_signal     516..1404
                     /label=Extended\packaging\signal
     misc_feature    1410..1433
                     /label=MCS
     promoter        1429..1937
                     /label=Phosphoglycerate\Kinase\Promoter
     CDS             1958..2557
                     /label=Puro(R)
     LTR             2687..3170
                     /label=3'\PCMV\LTR
BASE COUNT     1407 a      1785 c      1666 g      1406 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg 
     1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca 
     1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc 
     1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga 
     1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc 
     1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg 
     1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg 
     1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg 
     1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct 
     1921 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt 
     1981 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 
     2041 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 
     2101 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 
     2161 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 
     2221 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 
     2281 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 
     2341 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 
     2401 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 
     2461 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 
     2521 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg 
     2581 cccgaccgaa aggagcgcac gaccccatgc atcgataaaa taaaagattt tatttagtct 
     2641 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac 
     2701 gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta 
     2761 ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 
     2821 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag 
     2881 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 
     2941 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat 
     3001 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg 
     3061 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct 
     3121 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag 
     3181 tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac 
     3241 tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga 
     3301 cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac 
     3361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 
     3421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 
     3481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     3541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     3601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     3661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     3721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     3781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     3841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     3901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     3961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     4021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     4081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     4141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     4201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     4261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     4321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     4381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     4441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     4501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     4561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     4621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     4681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     4741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     4801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     4861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     4921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     4981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     5041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     5101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     5161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     5221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     5281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     5341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     5401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
     5461 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 
     5521 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 
     5581 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 
     5641 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 
     5701 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 
     5761 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 
     5821 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 
     5881 acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca 
     5941 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac 
     6001 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat 
     6061 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga 
     6121 ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc 
     6181 aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa aagattttat 
     6241 ttagtctcca gaaaaagggg ggaa 
//

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pMSCV-puro载体应用举例

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