pLVX-CherryPicker Control

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-CherryPicker Control
质粒类型: 慢病毒载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8998 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: CherryPicker (C-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-CherryPicker Control是pLVX-CherryPicker1和pLVX-CherryPicker2的阳性对照载体。
产品目录号: 632580
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-CherryPicker Control载体图谱



pLVX-CherryPicker Control 载体特征

载体简介

载体序列

LOCUS       pLVX-CherryPicker_Control   8999 bp    DNA   circular            11-APR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632580,632574,632581,632575
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4967..5558
                     /label=WPRE
     rep_origin      complement(6867..7540)
                     /label=pUC\origin
     CDS             complement(7685..8681)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5761..6397
                     /label=3'\LTR
     misc_feature    3825..4333
                     /label=PGK\Promoter
     misc_feature    4354..4953
                     /label=Puro(R)
     CDS             2885..3811
                     /label=CherryPicker
     misc_feature    3812..3814
                     /label=Stop\codon
BASE COUNT     2264 a      2310 c      2406 g      2019 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccgcg gccgcgttta 
     2881 aacgatgatg gtggacggcg acaacagcca cgtggagatg aagctggccg tggacgagga 
     2941 agagaacgcc gacaacaaca ccaaggccaa cgtgaccaag cccaagcggt gcagcggcag 
     3001 catctgctac ggcacaatcg ccgtgatcgt gttcttcctg atcggcttca tgatcggcta 
     3061 cctgggctac tgcaagtcta gcgacggccc tggcgagaca ggcatggtgt ccaagggcga 
     3121 agaggacaac atggccatca tcaaagaatt catgcggttc aaggtgcaca tggaaggcag 
     3181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgaa ggcagacctt acgagggcac 
     3241 ccagaccgcc aagctgaaag tgaccaaggg cggacccctg ccctttgcct gggacatcct 
     3301 gagcccccag ttcatgtacg gcagcaaggc ctacgtgaag caccccgccg acatccccga 
     3361 ctacctgaag ctgagcttcc ccgagggctt caagtgggag agagtgatga acttcgagga 
     3421 cggcggcgtg gtcaccgtga cccaggacag cagcctgcag gacggcgagt tcatctacaa 
     3481 agtgaagctg cggggcacca acttccctag cgacggcccc gtgatgcaga aaaagaccat 
     3541 gggctgggag gccagcagcg agagaatgta ccccgaggat ggcgccctga agggcgagat 
     3601 caagcagcgg ctgaagctga aggatggcgg ccactacgac gccgaagtga aaaccaccta 
     3661 caaggccaag aaacccgtgc agctgcctgg cgcctacaac gtgaacatca agctggacat 
     3721 caccagccac aacgaggact acaccatcgt ggagcagtac gagagagccg agggcagaca 
     3781 cagcaccggc ggcatggacg agctgtacaa atgacaatct agataattct accgggtagg 
     3841 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 
     3901 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 
     3961 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 
     4021 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 
     4081 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 
     4141 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 
     4201 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 
     4261 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 
     4321 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 
     4381 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 
     4441 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 
     4501 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 
     4561 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 
     4621 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 
     4681 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 
     4741 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 
     4801 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 
     4861 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 
     4921 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 
     4981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 
     5041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 
     5101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 
     5161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 
     5221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 
     5281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 
     5341 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 
     5401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 
     5461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 
     5521 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 
     5581 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 
     5641 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 
     5701 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 
     5761 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 
     5821 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 
     5881 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 
     5941 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 
     6001 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 
     6061 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 
     6121 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 
     6181 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 
     6241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 
     6301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 
     6361 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 
     6421 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 
     6481 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 
     6541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     6601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     6661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     6721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     6781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     6841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     6901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     6961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     7021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     7081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     7141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     7201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     7261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     7321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 
     7381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     7441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     7501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     7561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     7621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     7681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     7741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     7801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     7861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     7921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     7981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     8041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     8101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     8161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     8221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     8281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     8341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     8401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     8461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     8521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     8581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     8641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 
     8701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     8761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     8821 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 
     8881 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 
     8941 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt 
//

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