pLVX-Het-2

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-Het-2
质粒类型: 慢病毒载体;双顺反子载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8902 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: DmrA
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-Het-2表达DmrA融合蛋白;两个MCS区。
产品目录号: 635075
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-Het-2载体图谱



pLVX-Het-2 多克隆位点

pLVX-Het-2 载体特征

载体简介

载体序列

LOCUS       pLVX-Het2    8902 bp    DNA   circular             8-NOV-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No.635074, 635075 
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=CTS/cppt\(DNA\flap)
     promoter        2185..2788
                     /label=pCMV
     misc_feature    4824..5415
                     /label=WPRE
     rep_origin      complement(6725..7395)
                     /label=pUC\origin
     CDS             complement(7540..8536)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             5619..6255
                     /label=LTR
     CDS             3776..4810
                     /label=Hyg(R)
     misc_feature    3177..3752
                     /label=IRES
     misc_feature    2822..3151
                     /label=DmrA
BASE COUNT     2294 a      2156 c      2299 g      2153 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 
     2821 tatgtctaga ggagtccagg tggaaaccat ctccccagga gacgggcgca ccttccccaa 
     2881 gcgcggccag acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga 
     2941 ttcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg 
     3001 aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc 
     3061 tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct 
     3121 cgtcttcgat gtggagcttc taaaactgga aagcggccgc ggatcctacg tatgaccgcc 
     3181 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 
     3241 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 
     3301 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 
     3361 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 
     3421 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 
     3481 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 
     3541 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg 
     3601 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca 
     3661 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg 
     3721 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccgtac caaagatgga 
     3781 tagatccgga aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt 
     3841 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt 
     3901 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa 
     3961 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga 
     4021 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac 
     4081 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat 
     4141 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca 
     4201 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt 
     4261 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga 
     4321 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt 
     4381 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga 
     4441 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt 
     4501 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc 
     4561 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt 
     4621 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc 
     4681 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga 
     4741 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc 
     4801 aaaggaatag acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga 
     4861 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     4921 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     4981 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     5041 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     5101 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     5161 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 
     5221 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     5281 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     5341 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     5401 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac 
     5461 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga 
     5521 ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 
     5581 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc 
     5641 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 
     5701 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca 
     5761 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct 
     5821 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga 
     5881 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca 
     5941 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc 
     6001 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt 
     6061 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 
     6121 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 
     6181 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 
     6241 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 
     6301 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag 
     6361 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     6421 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     6481 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     6541 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     6601 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     6661 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     6721 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     6781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     6841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     6901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     6961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     7021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     7081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     7141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     7201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     7261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     7321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     7381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     7441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     7501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     7561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     7621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     7681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     7741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     7801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     7861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     7921 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     7981 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     8041 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     8101 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     8161 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     8221 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     8281 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     8341 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     8401 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     8461 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     8521 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt 
     8581 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     8641 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc 
     8701 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc 
     8761 tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat 
     8821 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa 
     8881 tatgtactac aaacttagta gt 
//

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