pMIB/V5-His B

价格:1250元

联系方式:I47-825O-882O

买家导航

pMIB-V5-HisB载体基本信息

出品公司: Invitrogen
载体名称: pMIB/V5-His B
质粒类型: 昆虫细胞表达系统载体
高拷贝/低拷贝: 高拷贝
启动子: OpIE2
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3600bp
5' 测序引物及序列: OpIE2-F: 5'd[CGCAACGATCTGGTAAACAC]3'
3' 测序引物及序列: --
载体标签: HBM (Nterm), V5 (Cterm), His (Cterm)
载体抗性: Ampicillin
筛选标记: Blasticidin
备注: 在昆虫细胞中能够稳定表达,HBM标签可实现目的蛋白的分泌表达
产品目录号: V803001
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 非病毒

pMIB-V5-HisB载体质粒图谱和多克隆位点信息

pMIB/V5-His B载体图谱



pMIB/V5-His B多克隆位点

pMIB/V5-His B载体特征

pMIB-V5-HisB载体简介

pMIB-V5-HisB载体序列

LOCUS       pMIB/V5-His B	3600 bp 	DNA	SYN
DEFINITION  pMIB/V5-His B
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="pMIB/V5-His B"
                     /mol_type="other DNA"
     misc_feature    511..530
                     /label="OpIE2_fwd_primer"
     rep_origin      complement(1066..1685)
                     /label="pBR322_origin"
     promoter        2059..2126
                     /label="EM7_promoter"
     gene            2127..2525
                     /label="blast (variant)"
                     /gene="blast (variant)"
     gene            2645..3505
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2645..3505
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG
   61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT
  121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC
  181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC
  241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC
  301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT
  361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA
  421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC
  481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG
  541 CATCTGTTCG AATTTAAAGC TACCATGAAA TTCTTAGTCA ACGTTGCCCT TGTTTTTATG
  601 GTCGTATACA TTTCTTACAT CTATGCCGGC ATGCTAAGCT TGGTACCGAG CTCGGATCCA
  661 CTAGTCCAGT GTGGTGGAAT TCTGCAGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG
  721 AGGGCCCGCG GTTCGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGC
  781 GTACCGGTCA TCATCACCAT CACCATTGAG TTTATCTGAC TAAATCTTAG TTTGTATTGT
  841 CATGTTTTAA TACAATATGT TATGTTTAAA TATGTTTTTA ATAAATTTTA TAAAATAATT
  901 TCAACTTTTA TTGTAACAAC ATTGTCCATT TACACACTCC TTTCAAGCGC GTGGGATCGA
  961 TGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
 1021 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
 1081 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
 1141 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
 1201 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
 1261 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
 1321 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
 1381 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
 1441 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
 1501 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
 1561 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
 1621 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
 1681 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
 1741 CATGCGAAAC ACGCACGGCG CGCGCACGCA GCTTAGCACA AACGCGTCGT TGCACGCGCC
 1801 CACCGCTAAC CGCAGGCCAA TCGGTCGGCC GGCCTCATAT CCGCTCACCA GCCGCGTCCT
 1861 ATCGGGCGCG GCTTCCGCGC CCATTTTGAA TAAATAAACG ATAACGCCGT TGGTGGCGTG
 1921 AGGCATGTAA AAGGTTACAT CATTATCTTG TTCGCCATCC GGTTGGTATA AATAGACGTT
 1981 CATGTTGGTT TTTGTTTCAG TTGCAAGTTG GCTGCGGCGC GCGCAGCACC TTTGCCGGGA
 2041 TCTGCCGGGC TGCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT
 2101 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC
 2161 TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG
 2221 CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA
 2281 CTGGGGGACC TTGCGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA
 2341 ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC
 2401 GGTGCCGACA GGTTCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG
 2461 ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG
 2521 GCTAAGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTCC CGGGAGATCT GCATGTCTAC
 2581 TAAACTCACA AATTAGAGCT TCAATTTAAT TATATCAGTT ATTACCCATT GAAAAAGGAA
 2641 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
 2701 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
 2761 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG
 2821 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT
 2881 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA
 2941 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
 3001 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
 3061 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG
 3121 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC
 3181 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT
 3241 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
 3301 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
 3361 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT
 3421 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG
 3481 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
 3541 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT

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