pFBDM

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pFBDM
质粒类型: 昆虫细胞表达系统载体
高拷贝/低拷贝: 高拷贝
启动子:
Polyhedrin,p10
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5279bp
5' 测序引物及序列:
p2Bac-F (for MCS1):ggacctttaattcaacccaacac
Polyhedrin-F (for MCS2):AAATGATAACCATCTCGC
 
3' 测序引物及序列:
TK-pA-R (for MCS1):TTGTCTCCTTCCGTGTTTCA
SV40pA-R (for MCS2):GAAATTTGTGATGCTATTGC
 
Genbank: DJ417502.1
载体标签:
载体抗性: 氨苄青霉素
筛选标记: 庆大霉素
备注: 可同时表达两种蛋白
产品目录号: --
稳定性: 稳定
组成型: 非组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

pFBDM载体图谱



pFBDM多克隆位点

载体简介

载体序列

LOCUS       pFBDM	5279 bp 	DNA   circular	SYN
DEFINITION  pFBDM
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="pFBDM"
                     /mol_type="other DNA"
     rep_origin      119..425
                     /label="f1_origin"
     promoter        619..647
                     /label="AmpR_promoter"
     gene            689..1549
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             689..1549
                     /label="ORF frame 2"
     rep_origin      1704..2323
                     /label="pBR322_origin"
     gene            complement(2902..3435)
                     /label="gent"
                     /gene="gent"
     CDS             complement(2902..3435)
                     /label="ORF frame 3"
     CDS             3537..4235
                     /label="ORF frame 3"
     terminator      complement(4016..4286)
                     /label="TK_PA_terminator"
     promoter        4521..4609
                     /label="PH_promoter"
     misc_feature    4535..4552
                     /label="polyhedrin_fwd_primer"
     terminator      4872..5003
                     /label="SV40_PA_terminator"
     misc_feature    4960..4979
                     /label="EBV_rev_primer"
ORIGIN
    1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
   61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
  121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
  181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
  241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
  301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
  361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
  421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
  481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
  541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
  601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
  661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
  721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
  781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
  841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
  901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
  961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
 1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
 1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
 1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
 1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
 1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
 1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
 1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
 1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
 1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
 1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
 1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
 1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
 1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
 1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
 1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
 1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
 1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
 2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
 2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
 2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
 2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
 2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
 2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
 2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
 2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
 2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
 2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
 2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
 2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
 2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
 2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
 2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
 2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
 3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
 3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
 3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
 3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
 3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
 3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
 3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
 3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
 3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
 3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
 3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
 3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
 3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
 3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
 3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
 3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
 3961 atagttctag tggttggcct acagctttgt ttaaacaaag ctgtacccgt agtggctatg
 4021 gcagggcttg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggt
 4081 tggggtgggg aaaaggaaga aacgcgggcg tattggtccc aatggggtct cggtggggta
 4141 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc
 4201 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
 4261 cttccgtgtt tcagttagcc tcccccatct cccggtaccg catgctatgc atcagctgct
 4321 agcaccatgg ctcgagatcc cgggtgatca agtcttcgtc gagtgattgt aaataaaatg
 4381 taatttacag tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa
 4441 ctataatata ttgtgttggg ttgaattaaa ggtccgtata ctagtatcga ttcgcgacct
 4501 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
 4561 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
 4621 attcataccg tcccaccatc gggcgcggat cccggtccga agcgcgcgga attcaaaggc
 4681 ctacgtcgac gagctcacta gtcgcggccg ctttcgaatc tagagcctgc agtctcgaca
 4741 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
 4801 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
 4861 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
 4921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
 4981 atgtatctta tcatgtctgg atctgatcac tgcttgagcc taggagatcc gaaccagata
 5041 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
 5101 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
 5161 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
 5221 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctacttt

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