| 质粒名称: | pMF111 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000585 |
| 产品用途: | Mammalian TetR-specific SEAP expression vector (PhCMV*-1-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | tetO7 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pMF111
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pMF111载体质粒基本信息
pMF111质粒图谱载体图谱和pMF111载体序列质粒序列多克隆位点信息
pMF111质粒载体简介
pMF111质粒序列载体序列
LOCUS pMF111 5377 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5377)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5377
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 2..295
/label=TET07
protein_bind 15..285
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
primer_bind complement(291..310)
/label=oYW06
misc_feature 296..444
/label=hcmvmin
primer_bind complement(428..445)
/label=oLZ158 TetO7-hcmvmin R
primer_bind complement(429..444)
/label=oYW11
misc_feature 467..491
/label=New Feature
CDS 493..2013
/codon_start=1
/label=SEAP
/translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPG"
polyA_signal 3057..3191
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3536..4164)
/direction=LEFT
/label=ColE1 origin
rep_origin complement(3554..4142)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4611..4989)
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
promoter complement(5174..5278)
/gene="bla"
/label=AmpR promoter
misc_feature 5350..5373
/label=New Feature
primer_bind 5350..5373
/label=oLZ157 TetO7-hcmvmin F
primer_bind 5353..5372
/label=oYW05
ORIGIN
1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
421 accgggaccg atccagcctc cgcggccccg aattcgagct cgcccgggga tcctctagag
481 tcagcttcct gcatgctgct gctgctgctg ctgctgggcc tgaggctaca gctctccctg
541 ggcatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc agccgaggcc
601 ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct catcatcttc
661 ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa agggcagaag
721 aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata tgtggctctg
781 tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc cacggcctac
841 ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc ccgctttaac
901 cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc caagaaagca
961 gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc agccggcacc
1021 tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc ctcggcccgc
1081 caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat tgacgtgatc
1141 ctaggtggag gccgaaagta catgtttcgc atgggaaccc cagaccctga gtacccagat
1201 gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga atggctggcg
1261 aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc ttccctggac
1321 ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata cgagatccac
1381 cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct gcgcctgctg
1441 agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga ccatggtcat
1501 catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga cgccattgag
1561 agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc cgaccactcc
1621 cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg gctggcccct
1681 ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg tccaggctat
1741 gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag ccccgagtat
1801 cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga cgtggcggtg
1861 ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac cttcatagcg
1921 cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct ggcgcccccc
1981 gccggcacca ccgacgccgc gcacccgggt taacccgtgg tccccgcgtt gcttcctctg
2041 ctggccggga ccctgctgct gctggagacg gccactgctc cctgagtgtc ccgtccctgg
2101 ggctcctgct tccccatccc ggagttctcc tgctccccac ctcctgtcgt cctgcctggc
2161 ctccagcccg agtcgtcatc cccggagtcc ctatacagag gtcctgccat ggaaccttcc
2221 cctccccgtg cgctctgggg actgagccca tgacaccaaa cctgcccctt ggctgctctc
2281 ggactcccta ccccaacccc agggactgca ggttgtgccc tgtggctgcc tgcaccccag
2341 gaaaggaggg ggctcaggcc atccagccac cacctacagc ccagtggcct cgagacctgc
2401 agcccaagct taaaacagct ctggggttgt acccacccca gaggcccacg tggcggctag
2461 tactccggta ttgcggtacc cttgtacgcc tgttttatac tcccttcccg taacttagac
2521 gcacaaaacc aagttcaata gaagggggta caaaccagta ccaccacgaa caagcacttc
2581 tgtttccccg gtgatgtcgt atagactgct tgcgtggttg aaagcgacgg atccgttatc
2641 cgcttatgta cttcgagaag cccagtacca cctcggaatc ttcgatgcgt tgcgctcagc
2701 actcaacccc agagtgtagc ttaggctgat gagtctggac atccctcacc ggtgacggtg
2761 gtccaggctg cgttggcggc ctacctatgg ctaacgccat gggacgctag ttgtgaacaa
2821 ggtgtgaaga gcctattgag ctacataaga atcctccggc ccctgaatgc ggctaatccc
2881 aacctcggag caggtggtca caaaccagtg attggcctgt cgtaacgcgc aagtccgtgg
2941 cggaaccgac tactttgggt gtccgtgttt ccttttattt tattgtggct gcttatggtg
3001 acaatcacag attgttatca taaagcgaat tggattgcgg ccgcaaagct agcttcgatc
3061 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
3121 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
3181 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
3241 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg
3301 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
3361 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
3421 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
3481 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatcgggccg
3541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
3661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
3781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
3841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
3901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
3961 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
4021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
4081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
4141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
4321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
4381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
4441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
4501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
4561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
4621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
4681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
4741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
4801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
4861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
4921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
4981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
5041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
5101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
5161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
5221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
5281 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
5341 ataaaaatag gcgtatcacg aggccctttc gtcttca
//
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