| 出品公司: | Clontech (TaKaRa) |
|---|---|
| 载体名称: | pTetone |
| 载体又名: | pTetone载体，pTetone质粒，pTetone载体质粒 |
| 英文名称: | pTetone, pTetone Vector, pTetone Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TRE3GS |
| 载体大小: | 4.2 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | pTet-One 3G载体套件是Tet-One™可诱导表达系统的一个组成部分，用于构建严格调控且高度敏感的四环素（Tet）可诱导哺乳动物表达系统，该系统可通过向培养基中添加多西环素来激活。pTetOne载体在正向方向上从组成型人磷酸甘油醛激酶（PGK）启动子组成型表达Tet-On® 3G反式激活因子，在反向方向上从PTRE3GS启动子表达您感兴趣的基因。 |
| 产品目录号: | 20268 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 相关载体: | pTetOne-Luc Control |
| 载体说明书: |
pTetOne载体说明书 pTetOne载体操作手册 pTetOne载体COA证书 |
pTetone
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pTetone载体质粒基本信息
pTetone质粒图谱载体图谱和pTetone载体序列质粒序列多克隆位点信息
pTetone质粒载体简介
pTetone质粒序列载体序列
Snapgene载体序列下载: pTetOne序列 .gb格式载体序列下载:pTetOne载体序列
LOCUS pTetOne 4207 bp DNA circular SYN 07-MAR-2024
DEFINITION All-in-one vector for strong inducible expression of genes in
mammalian cells using the Tet-On(R) system.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4207)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Jun 21, 2026 from SnapGene 8.1.0
https://www.biofeng.com
COMMENT Sequence Label: pTetOne
FEATURES Location/Qualifiers
source 1..4207
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(1..368)
/label=TRE3GS promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, modified to eliminate
binding sites for endogenous mammalian transcription
factors"
/note="color: #ffffff; direction: LEFT"
protein_bind 126..144
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
/note="color: #31849b"
protein_bind 162..180
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
/note="color: #31849b"
protein_bind 198..216
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
/note="color: #31849b"
protein_bind 234..252
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
/note="color: #31849b"
protein_bind 270..288
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
/note="color: #31849b"
protein_bind 306..324
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
/note="color: #31849b"
protein_bind 342..360
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
/note="color: #31849b"
misc_feature 372..463
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
/note="color: #a6acb3"
polyA_signal 477..525
/note="synthetic polyadenylation signal"
/note="color: #a6acb3"
promoter 545..1055
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 1074..1820
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/note="color: #993366"
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
polyA_signal 1937..2018
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
rep_origin complement(2200..2788)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(join(2959..3750,3751..3819))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 2959 .. 3750 / #ccffcc
2: 3751 .. 3819 / #ccffcc / signal sequence
Cleavage site after base 3750"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3820..3924)
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: LEFT"
polyA_signal 3957..4038
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
misc_feature 4158..4207
/label=MCS
/note="multiple cloning site"
/note="color: #99ccff"
ORIGIN
1 tttacgaggg taggaagtgg tacggaaagt tggtataaga caaaagtgtt gtggaattga
61 agtttactca aaaaatcagc actcttttat aggcgccctg gtttacataa gcaaagctta
121 tacgttctct atcactgata gggagtaaac tggatatacg ttctctatca ctgataggga
181 gtaaactgta gatacgttct ctatcactga tagggagtaa actggtcata cgttctctat
241 cactgatagg gagtaaactc cttatacgtt ctctatcact gatagggagt aaagtctgca
301 tacgttctct atcactgata gggagtaaac tcttcatacg ttctctatca ctgataggga
361 gtaaactcga gagagaaatg ttctggcacc tgcacttgca ctggggacag cctattttgc
421 tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta tcgattcaca
481 caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattctcga ggtgataatt
541 ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
601 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
661 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
721 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
781 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
841 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
901 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
961 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
1021 ccctcgttga ccgaatcacc gacctctctc cccaggggga tcatcgaatt accatgtcta
1081 gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg
1141 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
1201 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
1261 atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
1321 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
1381 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
1441 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
1501 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
1561 ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg
1621 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
1681 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
1741 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
1801 ttgacatgct ccccgggtaa atctccagag gatcataatc agccatacca catttgtaga
1861 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
1921 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
1981 catcacaaat ttcacaaata aagcattttt ttcactgccc cgagcttcct cgctcactga
2041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
2101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
2161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
2221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
2281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
2341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
2401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
2461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
2521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
2581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
2641 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
2701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
2761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
2821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
2881 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
2941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
3001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
3061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
3121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
3181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
3241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
3301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
3361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
3421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
3481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
3541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
3601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
3661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
3721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
3781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
3841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
3901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag
3961 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
4021 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
4081 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
4141 gatcctctag acatatgctg cagagatctg gatccaccgg tatcgatacg cgtgcggccg
4201 cgaattc
//
pTetone载体图谱质粒图谱pdf版和pTetone载体序列质粒序列等相关资料下载
pTetone质粒载体应用举例
pTetone-Luc Control
2026-06-21
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