| 质粒名称: | pSLCAR-CD19-BBz |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 135992 |
| 存储实验室: | Scott McComb |
|
载体骨架基本信息 |
|
| 载体名称: | pSL |
| 载体出品公司: | Heavily modified from LentiCRISPR v1 backbone |
| 载体抗性: | 氨苄青霉素 |
| 载体修饰: | 使用BpiI单锅限制性连接将CAR的抗原结合域从CD19交换到所需的特异性 |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FMC63-41BB-3z-P2A-EGFP,CD19(FMC63)-CAR, 41BB costim., CD3z stim domains, P2A-GFP marker,Contains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, 41BB-ITD, CD3z-ITD |
| 物种来源: | H. sapiens (human) |
| 启动子 | EF1a-short |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BpiI（在克隆过程中被破坏） |
| 3' 克隆位点: | BpiI（在克隆过程中被破坏） |
| 5' 测序引物 | CGGGTTTGCCGCCA |
| 3' 测序引物 | caggagccTGCTCCTCTTACTcc |
|
文献引用方法 |
|
| 材料和方法部分: | pSLCAR-CD19-BBz was a gift from Scott McComb (Addgene plasmid # 135992 ; http://n2t.net/addgene:135992 ; RRID:Addgene_135992) |
| 参考文献部分: | A High-Throughput Method for Characterizing Novel Chimeric Antigen Receptors in Jurkat Cells. Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K, Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon RA, Gilbert R, Marcil A, Weeratna RD, McComb S. Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi: 10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13. 10.1016/j.omtm.2020.01.012 PubMed 32083149 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSLCAR-CD19-BBz 8872 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Modular 41BB-CD3z CAR backbone, For Transient Expression or
Lentiviral Production.
ACCESSION .
VERSION .
KEYWORDS pSLCAR-CD19-BBz
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8872)
AUTHORS Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K,
Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon
RA, Gilbert R, Marcil A, Weeratna RD, McComb S
TITLE A High-Throughput Method for Characterizing Novel Chimeric Antigen
Receptors in Jurkat Cells.
JOURNAL Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi:
10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13.
PUBMED 32083149
REFERENCE 2 (bases 1 to 8872)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8872
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1443..1676
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1861..1905
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2128..2245
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2271..2482
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 2571..2636
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 4107..4163
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4164..4880
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 4164..4877
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(4206..4227)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4467..4486)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4814..4835
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4895..5483
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4948..4968)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5366..5377)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5555..5788
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5866..5987
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5903..5922)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5957..5976
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6027..6162
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6089..6108
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6182..6201)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6183..6201)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6211..6228)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6211..6227)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6220..6242)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6369..6824
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6456..6475)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6666..6687
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6850..6954
/gene="bla"
/label=AmpR promoter
CDS 6955..7815
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7173..7192)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7986..8574
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8475..8494
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(8682..8701)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2041 ttatcgtttc agacccacct cccaaccccg aggggacccc gggtttatta cagggacagc
2101 agagatccac tttggcgccg gctcgagttt taaaagaaaa ggggggattg gggggtacag
2161 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
2221 acaaattaca aaaattcaaa atttttcgag tggctccggt gcccgtcagt gggcagagcg
2281 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
2341 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
2401 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
2461 cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg ctcaggctgc
2521 tcttggctct caacttattc ccttcaattc aagtaacagg agggtcttcg gactacaagg
2581 atcatgacgg agactataag gatcacgata ttgattacaa agatgacgac gacaaagaca
2641 tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga gtcaccatca
2701 gttgcagggc aagtcaggac atctctaagt atttgaattg gtatcagcag aaaccagatg
2761 gaactgttaa actcctgatc taccatacat caagattaca ctcaggagtc ccatcaaggt
2821 tcagtggcag tgggtctgga acagattatt ctctcaccat tagcaacctg gagcaagaag
2881 atattgccac ttacttttgc caacagggta atacgcttcc gtacacgttc ggagggggga
2941 ctaagttgga aataacaggc tccacctctg gatccggcaa gcccggatct ggcgagggat
3001 ccaccaaggg cgaggtgaaa ctgcaggagt caggacctgg cctggtggcg ccctcacaga
3061 gcctgtccgt cacatgcact gtctcagggg tctcattacc cgactatggt gtaagctgga
3121 ttcgccagcc tccacgaaag ggtctggagt ggctgggagt aatatggggt agtgaaacca
3181 catactataa ttcagctctc aaatccagac tgaccatcat caaggacaac tccaagagcc
3241 aagttttctt aaaaatgaac agtctgcaaa ctgatgacac agccatttac tactgtgcca
3301 aacattatta ctacggtggt agctatgcta tggactactg gggtcaagga acctcagtca
3361 ccgtctcctc agcggccgca ggtaccacca caacgcccgc tcctcggcca ccgacgccag
3421 cgccaactat tgcgagtcag cctctcagtc tgcgacctga ggcttgtcga ccagcagccg
3481 gaggcgcagt gcacacgagg gggctggact tcgcctgtga tagaagacct ccttctaagc
3541 ccttttgggt gctggtggtg gttggtggag tcctggcttg ctatagcttg ctagtaacag
3601 tggcctttat tattttctgg gtgaggaaac ggggcagaaa gaaactcctg tatatattca
3661 aacaaccatt tatgagacca gtacaaacta ctcaagagga agatggctgt agctgccgat
3721 ttccagaaga agaagaagga ggatgtgaac tggctagcct gagagtgaag ttcagcagga
3781 gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag ctcaatctag
3841 gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct gagatggggg
3901 gaaagccgca gagaaggaag aaccctcagg aaggcctgta caatgaactg cagaaagata
3961 agatggcgga ggcctacagt gagattggga tgaaaggcga gcgccggagg ggcaaggggc
4021 acgatggcct ttaccagggt ctcagtacag ccaccaagga cacctacgac gcccttcaca
4081 tgcaggccct gccccctcgc gctagcgcca cgaacttctc tctgttaaag caagcaggcg
4141 acgtggaaga aaaccccggt cccgtgagca agggcgagga gctgttcacc ggggtggtgc
4201 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
4261 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
4321 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
4381 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
4441 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
4501 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
4561 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
4621 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
4681 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
4741 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
4801 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
4861 tggacgagct gtacaagtaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
4921 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
4981 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
5041 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
5101 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
5161 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
5221 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
5281 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
5341 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
5401 cctgctgccg gctctgcggc ctcttccgcg tctttgcctt cgccctcaga cgagtcggat
5461 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
5521 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
5581 agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
5641 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
5701 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
5761 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
5821 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
5881 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
5941 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
6001 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
6061 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
6121 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
6181 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
6241 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
6301 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
6361 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
6421 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
6481 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
6541 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
6601 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
6661 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
6721 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
6781 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
6841 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
6901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
6961 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
7021 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
7081 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
7141 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
7201 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
7261 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
7321 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
7381 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
7441 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
7501 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
7561 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
7621 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
7681 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
7741 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
7801 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
7861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
7921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
7981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
8041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
8101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
8161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
8221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
8281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
8341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
8401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
8461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
8521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
8581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtgtcga
8641 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
8701 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
8761 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
8821 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
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