| 质粒名称: | pAAV-dMito-PSmOrange3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000580 |
| 产品用途: | Labels mitochondria with PSmOrange3 |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | COX8, PSmOrange3 |
| 物种来源: | H.sapiens, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking.
Subach O.M., Olumayowa F., Tang M., Qiao Y., Zhang Y., Vlaskina A.V., Nikolaeva A. Y., Kononikhin A. S., Agapova Y.K., Belousov A., Borshchevskiy V., Samygina V. R., Perfilov M. M., Mishin A. S., Piatkevich K. D., Subach F. V.
Sci. Reports 2025 151 15, 40166- (2025).
|
pAAV-dMito-PSmOrange3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-dMito-PSmOrange3载体质粒基本信息
pAAV-dMito-PSmOrange3质粒图谱载体图谱和pAAV-dMito-PSmOrange3载体序列质粒序列多克隆位点信息
pAAV-dMito-PSmOrange3质粒载体简介
pAAV-dMito-PSmOrange3质粒序列载体序列
LOCUS pAAV-dMito-PSmOrange3 5640 bp DNA circular SYN 04-JUL-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5640)
AUTHORS 1
TITLE Direct Submission
JOURNAL Exported Jul 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..5640
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
CDS 3649..3723
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 3751..3855
/codon_start=1
/label=Mito2
/translation="SVLTPLLLRGLTGSARRLPVPRAKIHSLGPARSAS"
CDS 3862..4569
/codon_start=1
/product="enhanced orange to far-red photoswitchable
monomeric fluorescent protein (Subach et al., 2012)"
/label=PSmOrange3
/note="mammalian codon-optimized"
/translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEVEGKGEGHPYEG
FQTVKLKVTKGGPLPFAWDILSPLVTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEVRMRLKLKDGGHYTSEAKTTYKAKKSVLLPGAYIVGIKLDITSHNEDFTIVEQYERS
EARHSTGGKDELYK"
misc_feature 4310..4315
/label=SDS
misc_feature 4678..4683
/label=SDS
CDS complement(5013..5024)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5173..5294
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature join(5640,1..940)
/label=ColE1
/note="ColE1"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccaccg gtgccaccat gtccgtcctg
3661 acgccgctgc tgctgcgggg cttgacaggc tcggcccggc ggctcccagt gccgcgcgcc
3721 aagatccatt cgttggggcc tgcaagatcc agtgtattaa cacctctgct gctgcggggc
3781 ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca agattcacag tcttggtcca
3841 gctagatctg ctagcctcga gatggtgagc aagggcgagg agaataatat ggccatcatt
3901 aaggagttca tgcgcttcaa ggtgcacatg gagggcactg tgaacggcca cgagttcgag
3961 gtcgagggca agggcgaggg ccacccctac gagggctttc agaccgttaa gctgaaggtg
4021 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctctcgt cacctacggc
4081 tccaaggcgt acgtgaagca ccccgccgac atccccgact acttcaagct gtccttcccc
4141 gagggattca agtgggagcg cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc
4201 caggactcct ctctgcagga cggcgagttc atctacaagg tgaagatgcg cggcaccaac
4261 ttcccctccg acgggcccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
4321 cggatgtacc ccgaggacgg cgccctgaag ggcgaggtca ggatgaggct gaagctgaag
4381 gatggcggcc actacacctc cgaggccaag actacctaca aggccaagaa gtccgtgctg
4441 ctgcccggcg cctacatcgt cggcatcaag ttggacatta cctcacacaa cgaggacttc
4501 accatcgtgg aacagtatga acgcagcgag gcccgccact ccaccggcgg caaggacgag
4561 ctgtacaagt aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
4621 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
4681 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
4741 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
4801 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
4861 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
4921 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
4981 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
5041 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
5101 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacccggg cggccgcttc
5161 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
5221 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
5281 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
5341 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
5401 ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa
5461 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
5521 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
5581 cgcagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
//
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