| 质粒名称: | H2B-mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20972 |
| 存储实验室: | Robert Benezra |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Histone cluster 1, H2bb,H2B.1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1100 bp |
| 基因突变: | -- |
| GenBank编号 | NM_021062 |
| 启动子 | -- |
| 标签/融合蛋白: | mCherry（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI (未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | Sp6 |
|
文献引用方法 |
|
| 材料和方法部分: | H2B-mCherry was a gift from Robert Benezra (Addgene plasmid # 20972 ; http://n2t.net/addgene:20972 ; RRID:Addgene_20972) |
| 参考文献部分: | High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442 |
买家导航
H2BmCherry载体基本信息
H2BmCherry载体图谱质粒图谱和多克隆位点信息
H2BmCherry载体简介
H2BmCherry载体序列
LOCUS H2B-mCherry 6476 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION H2B fused to mCherry for chromatin visualization.
ACCESSION .
VERSION .
KEYWORDS H2B-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6476)
AUTHORS Nam HS, Benezra R
TITLE High levels of Id1 expression define B1 type adult neural stem
cells.
JOURNAL Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
PUBMED 19896442
REFERENCE 2 (bases 1 to 6476)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6476
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 213..233
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin complement(242..670)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(352..373)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 564..583
/label=F1ori-R
/note="F1 origin, reverse primer"
polyA_signal 716..940
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 929..946
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
promoter 966..984
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 966..983
/label=SP6
/note="SP6 promoter, forward primer"
CDS complement(1000..1710)
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1278..1297)
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 1547..1565
/label=mCherry-R
/note="mCherry, reverse primer"
regulatory 1707..1716
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS complement(1732..2106)
/codon_start=1
/gene="HIST1H2BB"
/product="human histone H2B"
/label=H2B
/translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAVTKYTSS"
primer_bind complement(2131..2150)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2132..2150)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(2195..2398)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(2224..2244)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 2399..2778
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 2950..2969
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 3044..3148
/gene="bla"
/label=AmpR promoter
CDS 3149..4009
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3367..3386)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4180..4768
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4669..4688
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4922..4939
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5056..5077
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5092..5122
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5130..5146
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5135..5157
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5154..5170
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5154..5170
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 5219..5340
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5230..5249)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5284..5303
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(5514..6308)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5625..5644)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 6235..6254
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(6375..228)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6389..48
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6443..6462)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
61 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
121 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
181 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat
241 taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
301 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
361 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
421 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
481 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
541 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
601 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
661 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
721 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
781 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
841 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
901 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgagct
961 ctagcattta ggtgacacta tagaataggg ccctctagat tacttgtaca gctcgtccat
1021 gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc
1081 gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg
1141 cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt
1201 cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga
1261 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc
1321 gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt
1381 caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga
1441 cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtacat
1501 gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg tcaccttcag
1561 cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc
1621 gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct tgatgatggc
1681 catgttatcc tcctcgccct tgctcaccat ggtggcgacc ggtggatcct tagagctagt
1741 gtacttggta actgccttag tgccctcgga cacagcatgc ttagccagct ccccaggcag
1801 cagcaggcgc acagccgtct gaatctccct ggaggtgatg gtcgagcgct tattgtagtg
1861 agccaggcga gaagcctcgc ccgcgatgcg ctcgaagatg tcgttgacga aggagttcat
1921 gatccccatg gccttggatg agatgccggt gtcggggtgg acctgcttca gaaccttgta
1981 cacatagata gaatagctct ccttgcggct gcgcttacgc ttcttaccat ccttcttctg
2041 cgccttagtg atagccttct tagaaccctt tttaggggct ggagcagact tagagggttc
2101 aggcatggtg gcggtaccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa
2161 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
2221 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
2281 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
2341 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
2401 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
2461 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
2521 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
2581 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
2641 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
2701 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
2761 tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac
2821 gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt
2881 aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc
2941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc
3001 tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
3061 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
3121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
3181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
3241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
3301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
3361 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
3421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
3481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
3541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
3601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
3661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
3721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
3781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
3841 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
3901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
3961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
4021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
4081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
4141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
4201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
4261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
4321 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
4381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
4441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
4501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
4561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
4621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
4681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
4741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
4801 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
4861 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
4921 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
4981 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
5041 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
5101 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
5161 cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac agacatgata
5221 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
5281 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
5341 ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
5401 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
5461 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
5521 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
5581 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
5641 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
5701 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
5761 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
5821 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
5881 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
5941 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
6001 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
6061 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
6121 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
6181 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
6241 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
6301 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atccgaaaat ggatatacaa
6361 gctcccggga gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
6421 aatagctcag aggcagaggc ggcctcggcc tctgcataaa taaaaaaaat tagtca
//
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