pTXB1

价格:850元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: NEB(New England Biolabs)
载体名称: pTXB1
质粒类型: 原核细胞表达载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6706bp
5' 测序引物及序列: T7 Fwd
3' 测序引物及序列: --
载体标签:  intein
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: --
产品目录号: N6707
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pTXB1载体图谱



pTXB1多克隆位点

pTXB1载体特征

载体简介

pTXB1 contains a mini-intein from the Mycobacterium xenopi gyrA gene (Mxe GyrA intein; 198 amino acid residues)  that has been modified to undergo thiol-induced cleavage at its N-terminus (3,5). The vector allows for the 
purification of a target protein without any extra amino acids by cloning into the NdeI and SapI sites. The target protein is fused at its C-terminus to a self-cleavable intein tag (~28 kDa) that contains the chitin 
binding domain (CBD, 6 kDa) allowing for affinity purification of the fusion precursor on a chitin column.

载体序列

      1  AACTACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT 
     51  GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 
    101  CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA 
    151  ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 
    201  TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 
    251  TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 
    301  CCTTGAGAGT TTTCGCCCCG AAGAACGTTC TCCAATGATG AGCACTTTTA 
    351  AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG 
    401  CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC 
    451  ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 
    501  GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 
    551  ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 
    601  GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA 
    651  TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 
    701  TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA 
    751  ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 
    801  CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 
    851  CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 
    901  CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC 
    951  GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 
   1001  CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TACCCCGGTT 
   1051  GATAATCAGA AAAGCCCCAA AAACAGGAAG ATTGTATAAG CAAATATTTA 
   1101  AATTGTAAAC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA 
   1151  TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA 
   1201  TCAAAAGAAT AGCCCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA 
   1251  GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG 
   1301  TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCAA ATCAAGTTTT 
   1351  TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC 
   1401  CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG 
   1451  GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC 
   1501  ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG 
   1551  CGCGTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 
   1601  ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 
   1651  GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 
   1701  TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 
   1751  GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 
   1801  ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 
   1851  ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 
   1901  GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 
   1951  ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 
   2001  CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 
   2051  CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 
   2101  GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 
   2151  CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 
   2201  TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 
   2251  GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 
   2301  CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 
   2351  CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC 
   2401  CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC TATGGTGCAC TCTCAGTACA 
   2451  ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA 
   2501  CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC 
   2551  GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA 
   2601  CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA 
   2651  CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GCAGCGATTC 
   2701  ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA 
   2751  GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT 
   2801  TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG 
   2851  GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG 
   2901  ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG 
   2951  GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCC 
   3001  GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA 
   3051  TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT 
   3101  TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT 
   3151  CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG 
   3201  CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG 
   3251  GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA 
   3301  GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA GTGAGCTAAC 
   3351  TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 
   3401  TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 
   3451  GCGTATTGGG CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC 
   3501  AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC 
   3551  CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG 
   3601  GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG 
   3651  ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC 
   3701  CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT 
   3751  CATTCAGCAT TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG 
   3801  CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA GATATTTATG 
   3851  CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA 
   3901  ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT 
   3951  CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC 
   4001  AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG 
   4051  CAATGGCATC CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG 
   4101  CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT CGACGCCGCT 
   4151  TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG 
   4201  ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG 
   4251  GTGGCAACGC CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC 
   4301  GCGGTTGGGA ATGTAATTCA GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC 
   4351  GCGTTTTCGC AGAAACGTGG CTGGCCTGGT TCACCACGCG GGAAACGGTC 
   4401  TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG TTACTGGTTT 
   4451  CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC 
   4501  CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC 
   4551  CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT 
   4601  TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCCGGCATGC CGCCCTTTCG 
   4651  TCTTCAAGAA TTAATTCCCA ATTCCCCAGG CATCAAATAA AACGAAAGGC 
   4701  TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG 
   4751  CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG 
   4801  CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGAA 
   4851  TTAATTCCCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG 
   4901  CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA 
   4951  AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC CCGGAGGGTG 
   5001  GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GAATTAATTC CCCAGGCATC 
   5051  AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT 
   5101  TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA 
   5151  TTTGAACGTT GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC 
   5201  CATAAACTGC CAGGAATTAA TTCCCCAGGC ATCAAATAAA ACGAAAGGCT 
   5251  CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC 
   5301  TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC 
   5351  AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGAAT 
   5401  TAATTCCCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC 
   5451  CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA 
   5501  ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG 
   5551  CGGGCAGGAC GCCCGCCATA AACTGCCAGG AATTGGGGAT CGGAATTAAT 
   5601  TCCCGGTTTA AACCGGGGAT CTCGATCCCG CGAAATTAAT ACGACTCACT 
   5651  ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA TAATTTTGTT 
   5701  TAACTTTAAG AAGGAGATAT ACATATGGCT AGCTCGCGAG TCGACGGCGG 
   5751  CCGCGAATTC CTCGAGGGCT CTTCCTGCAT CACGGGAGAT GCACTAGTTG 
   5801  CCCTACCCGA GGGCGAGTCG GTACGCATCG CCGACATCGT GCCGGGTGCG 
   5851  CGGCCCAACA GTGACAACGC CATCGACCTG AAAGTCCTTG ACCGGCATGG 
   5901  CAATCCCGTG CTCGCCGACC GGCTGTTCCA CTCCGGCGAG CATCCGGTGT 
   5951  ACACGGTGCG TACGGTCGAA GGTCTGCGTG TGACGGGCAC CGCGAACCAC 
   6001  CCGTTGTTGT GTTTGGTCGA CGTCGCCGGG GTGCCGACCC TGCTGTGGAA 
   6051  GCTGATCGAC GAAATCAAGC CGGGCGATTA CGCGGTGATT CAACGCAGCG 
   6101  CATTCAGCGT CGACTGTGCA GGTTTTGCCC GCGGAAAACC CGAATTTGCG 
   6151  CCCACAACCT ACACAGTCGG CGTCCCTGGA CTGGTGCGTT TCTTGGAAGC 
   6201  ACACCACCGA GACCCGGACG CCCAAGCTAT CGCCGACGAG CTGACCGACG 
   6251  GGCGGTTCTA CTACGCGAAA GTCGCCAGTG TCACCGACGC CGGCGTGCAG 
   6301  CCGGTGTATA GCCTTCGTGT CGACACGGCA GACCACGCGT TTATCACGAA 
   6351  CGGGTTCGTC AGCCACGCTA CTGGCCTCAC CGGTCTGAAC TCAGGCCTCA 
   6401  CGACAAATCC TGGTGTATCC GCTTGGCAGG TCAACACAGC TTATACTGCG 
   6451  GGACAATTGG TCACATATAA CGGCAAGACG TATAAATGTT TGCAGCCCCA 
   6501  CACCTCCTTG GCAGGATGGG AACCATCCAA CGTTCCTGCC TTGTGGCAGC 
   6551  TTCAATGACT GCAGGAAGGG GATCCGGCTG CTAACAAAGC CCGAAAGGAA 
   6601  GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 
   6651  GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT 
   6701  CCGGAT

载体质粒图谱pdf版和相关资料下载

载体应用举例

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