| 质粒名称: | pAY5 (mAID-EGFP-NLS piggyBac) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 140532 |
| 存储实验室: | Masato Kanemaki |
|
载体骨架基本信息 |
|
| 载体名称: | pPB-LR5 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 8447 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | 潮霉素 |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mAID-EGFP-NLS |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAY5 (mAID-EGFP-NLS piggyBac) was a gift from Masato Kanemaki (Addgene plasmid # 140532 ; http://n2t.net/addgene:140532 ; RRID:Addgene_140532) |
| 参考文献部分: | The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT. Nat Commun. 2020 Nov 11;11(1):5701. doi: 10.1038/s41467-020-19532-z. 10.1038/s41467-020-19532-z PubMed 33177522 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pAY5 (mAID-EGFP-NLS piggyBac) 8447 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mAID-EGFP-NLS piggyBac.
ACCESSION .
VERSION .
KEYWORDS pAY5 (mAID-EGFP-NLS piggyBac)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8447)
AUTHORS Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano
R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y,
Hayashi KI, Kanemaki MT.
TITLE The auxin-inducible degron 2 technology provides sharp degradation
control in yeast, mammalian cells, and mice.
JOURNAL Nat Commun (2020) 11(1):5701
REFERENCE 2 (bases 1 to 8447)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8447
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(140..161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 352..371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
repeat_region 674..708
/label=piggyBac left (5') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
promoter 1154..2335
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1384..2326
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 2283..2303
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 2400..2603
/codon_start=1
/product="minimal degron from the auxin-responsive IAA17
protein of Arabidopsis thaliana (Kubota et al., 2013)"
/label=mini-AID
/note="acts as an auxin-inducible degron (AID) in the
presence of TIR1"
/translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
AAFVKVSMDGAPYLRKIDLRMYK"
CDS 2610..3326
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2610..3326
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2655..2676)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2916..2935)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3263..3284
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3363..3383
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 3519..3640
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3556..3575)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3610..3629
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind complement(3671..3704)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter 3765..4264
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(3800..3823)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4197..4216
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 4325..5344
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
protein_bind complement(5907..5940)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
repeat_region 6146..6208
/label=piggyBac right (3') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
primer_bind complement(6259..6279)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(6259..6277)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6298..6314)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6298..6314)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6311..6333)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6322..6338
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6346..6376)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6391..6412
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6529..6546)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6700..7288)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6780..6799)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7459..8319)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8082..8101
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8320..8424)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
961 atattttctt gttatagata tcaactagaa tgctagtaat gagtaattca tacaaaagga
1021 ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa cgtagaaccc
1081 agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg aggtggagaa
1141 gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
1201 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
1261 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
1321 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
1381 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
1441 gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg
1501 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
1561 ttgagttgag gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
1621 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
1681 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
1741 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
1801 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
1861 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
1921 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
1981 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
2041 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
2101 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
2161 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
2221 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
2281 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacgcta
2341 gcccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta cccaccatga
2401 aggagaagag tgcttgtcct aaagatccag ccaaacctcc ggccaaggca caagttgtgg
2461 gatggccacc ggtgagatca taccggaaga acgtgatggt ttcctgccaa aaatcaagcg
2521 gtggcccgga ggcggcggcg ttcgtgaagg tatcaatgga cggagcaccg tacttgagga
2581 aaatcgattt gaggatgtat aaagctagca tggtgagcaa gggcgaggag ctgttcaccg
2641 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
2701 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
2761 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
2821 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
2881 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
2941 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
3001 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
3061 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
3121 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
3181 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
3241 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
3301 ctctcggcat ggacgagctg tacaagggtg caggcgccca agcttcgaat tctgcagtcg
3361 accccaagaa gaaaagaaag gtcaggtagg gatccaccgg atctagataa ctgatcataa
3421 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
3481 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
3541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
3601 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtcgat catattcaat
3661 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag
3721 tttacgtcca gccaagctta ggatctcgac ctcgaaattc taccgggtag gggaggcgct
3781 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
3841 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cgactccgtt
3901 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
3961 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
4021 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
4081 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
4141 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
4201 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
4261 ttcgacctgc atccatctag atctcgagca gctgaagctt accgctagca tggatagatc
4321 cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
4381 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
4441 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
4501 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
4561 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
4621 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
4681 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
4741 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
4801 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
4861 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
4921 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
4981 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
5041 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
5101 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
5161 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
5221 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
5281 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
5341 ataggctagc cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
5401 tgcatcgatg atatcagatc cccgggatgc agaaattgat gatctattaa acaataaaga
5461 tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta
5521 cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa
5581 atgcctgctc tttactgaag gctctttact attgctttat gataatgttt catagttgga
5641 tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat
5701 ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt
5761 tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc
5821 ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa
5881 gctggtcgag atccggaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
5941 taggtccctc gaagaggttc actagctagt taaaagtttt gttactttat agaagaaatt
6001 ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta
6061 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
6121 cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt
6181 acgtcacaat atgattatct ttctagggtt aatctagtat acgcgtatgc ggccgcttaa
6241 ttaatccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
6301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
6361 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
6421 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
6481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
6541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
6601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
6661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
6721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
6781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
6841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
6901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
6961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
7021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
7081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
7141 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
7201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
7261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
7321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
7381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
7441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
7501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
7561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
7621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
7681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
7741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
7801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
7861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
7921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
7981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
8041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
8101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
8161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
8221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
8281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
8341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
8401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
