pLenti CMV/TO Neo empty (w215-1)

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

质粒名称: pLenti CMV/TO Neo empty (w215-1) 
出品公司: Addgene
目录编号: 17485
存储实验室: Eric Campeau

载体骨架基本信息
载体名称: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
载体抗性: 氨苄霉素
空载体大小: 8186 bp
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
克隆方法: 限制性内切酶
5' 克隆位点: Xho I(未破坏)
3' 克隆位点: Xba I(未破坏)
5' 测序引物 CMVforw
3' 测序引物  

文献引用方法
材料和方法部分: pLenti CMV/TO Neo empty (w215-1) was a gift from Eric Campeau (Addgene plasmid # 17485)
参考文献部分: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394

载体质粒图谱和多克隆位点信息

pLenti CMV/TO Neo empty (w215-1)载体图谱

载体简介

载体序列

LOCUS       pLenti CMV/TO Neo empty (w215-1)	8186 bp 	DNA	SYN
DEFINITION  pLenti CMV/TO Neo empty (w215-1)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="pLenti CMV/TO Neo empty (w215-1)"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             132..992
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     CDS             3168..3668
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /label="RRE"
     CDS             3629..4171
                     /label="ORF frame 2"
     misc_feature    4016..4031
                     /label="cPPT"
     promoter        4149..4653
                     /label="CMV_immearly_promoter"
     misc_feature    4156..4443
                     /label="CAG_enhancer"
     misc_feature    4610..4630
                     /label="CMV_fwd_primer"
     misc_feature    4661..4700
                     /label="tetO"
     misc_feature    4741..4765
                     /label="attB1"
     misc_feature    4834..4858
                     /label="attB2"
     misc_feature    4891..5466
                     /label="WPRE"
     CDS             4979..5512
                     /label="ORF frame 2"
     misc_feature    complement(5555..5578)
                     /label="MSCV_rev_primer"
     misc_feature    5952..5971
                     /label="mPGK_F_primer"
     gene            6033..6833
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             6033..6836
                     /label="ORF frame 3"
     misc_feature    6910..6925
                     /label="cPPT"
     misc_feature    6910..6931
                     /label="U3PPT"
     misc_feature    6980..7160
                     /label="HIV-1_5_LTR"
     misc_feature    6980..7160
                     /label="truncHIV-1_3_LTR"
     terminator      7235..7354
                     /label="SV40_PA_terminator"
     misc_feature    7323..7342
                     /label="EBV_rev_primer"
     rep_origin      7372..7449
                     /label="SV40_origin"
     misc_feature    7434..7453
                     /label="SV40pro_F_primer"
     promoter        complement(7540..7558)
                     /label="T7_promoter"
     promoter        complement(7568..7584)
                     /label="M13_forward20_primer"
     misc_feature    complement(7577..7599)
                     /label="M13_pUC_fwd_primer"
     misc_feature    7577..7720
                     /label="lacZ_a"
     rep_origin      7746..8052
                     /label="f1_origin"
ORIGIN
    1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
   61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
  121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
  181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
  241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
  301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
  361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
  421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
  481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
  541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
  601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
  661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
  721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
  781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
  841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
  901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
  961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
 4321 tatCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
 4381 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
 4441 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
 4501 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
 4561 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
 4621 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCCCTATCAG TGATAGAGAT
 4681 CTCCCTATCA GTGATAGAGA TCGTCGACTA GTCCAGTGTG GTGGAATTCT GCAGATATCA
 4741 ACAAGTTTGT ACAAAAAAGC AGGCTCCACC ATGGGAACCA ATTCAGTCGA CTGGATCCGG
 4801 TACCGAATTC GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG
 4861 ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa tttgtgaaag
 4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
 4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
 5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
 5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
 5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
 5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
 5281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
 5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
 5401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
 5461 ttgggccgcc tccccgcctg gAATTCTGCA GATATCCGGA CTAGTGATCT AATTCTACCG
 5521 GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG
 5581 CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG
 5641 CCAACCGGCT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG
 5701 GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG
 5761 TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG
 5821 GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG
 5881 TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC
 5941 CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC
 6001 CTCATCTCCG GGCCTTTCGA CCTGCAGCCA ATATGGGATC GGCCATTGAA CAAGATGGAT
 6061 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
 6121 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
 6181 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
 6241 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
 6301 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
 6361 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
 6421 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
 6481 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
 6541 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
 6601 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
 6661 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
 6721 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
 6781 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGGGG
 6841 ATCCGTCGAG GGCCCGGTAC ctttaagacc aatgacttac aaggcagctg tagatcttag
 6901 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
 6961 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
 7021 tggctgccta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
 7081 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
 7141 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
 7201 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
 7261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
 7321 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
 7381 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
 7441 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
 7501 ggaggcctag gcttttgcgt cgagacgtac ccaattcgcc ctatagtgag tcgtattacg
 7561 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
 7621 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
 7681 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta
 7741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
 7801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
 7861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
 7921 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
 7981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
 8041 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
 8101 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
 8161 acaaaatatt aacgtttaca atttcc
//

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