| 质粒名称: |
pAAVLINK-EF1a-HA-5'-HIVEP2
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001689 |
| 产品用途: | 5' AAVLINK plasmid for HIVEP2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | HIVEP2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-HIVEP2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-HIVEP2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-HIVEP2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-HIVEP2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-HIVEP2质粒载体简介
pAAVLINK-EF1a-HA-5'-HIVEP2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-HIVEP2 6887 bp DNA circular SYN 19-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6887)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6887
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4027
/codon_start=1
/label=HIVEP2
/translation="DTGDTALGQKATSRSGETDKASGRWRQEQSAVIKMSTFGSHEGQR
QPQIEPEQIGNTASAQLFGSGKLASPSEVVQQVAEKQYPPHRPSPYSCQHSLSFPQHSL
PQGVMHSTKPHQSLEGPPWLFPGPLPSVASEDLFPFPIHGHSGGYPRKKISSLNPAYSQ
YSQKSIEQAEEAHKKEHKPKKPGKYICPYCSRACAKPSVLKKHIRSHTGERPYPCIPCG
FSFKTKSNLYKHRKSHAHAIKAGLVPFTESAVSKLDLEAGFIDVEAEIHSDGEQSTDTD
EESSLFAEASDKMSPGPPIPLDIASRGGYHGSLEESLGGPMKVPILIIPKSGIPLPNES
SQYIGPDMLPNPSLNTKADDSHTVKQKLALRLSEKKGQDSEPSLNLLSPHSKGSTDSGY
FSRSESAEQQISPPNTNAKSYEEIIFGKYCRLSPRNALSVTTTSQERAAMGRKGIMEPL
PHVNTRLDVKMFEDPVSQLIPSKGDVDPSQTSMLKSTKFNSESRQPQIIPSSIRNEGKL
YPANFQGSNPVLLEAPVDSSPLIRSNSVPTSSATNLTIPPSLRGSHSFDERMTGSDDVF
YPGTVGIPPQRMLRRQAAFELPSVQEGHVEVEHHGRMLKGISSSSLKEKKLSPGDRVGY
DYDVCRKPYKKWEDSETPKQNYRDISCLSSLKHGGEYFMDPVVPLQGVPSMFGTTCENR
KRRKEKSVGDEEDTPMICSSIVSTPVGIMASDYDPKLQMQEGVRSGFAMAGHENLSHGH
TERFDPCRPQLQPGSPSLVSEESPSAIDSDKMSDLGGRKPPGNVISVIQHTNSLSRPNS
FERSESAELVACTQDKAPSPSETCDSEISEAPVSPEWAPPGDGAESGGKPSPSQQVQQQ
SYHTQPRLVRQHNIQVPEIRVTEEPDKPEKEKEAQSKEPEKPVEEFQWPQRSETLSQLP
AEKLPPKKKRLRLADMEHSSGESSFESTGTGLSRSPSQESNLSHSSSFSMSFEREETSK
LSALPKQDEFGKHSEFLTVPAGSYSLSVPGHHHQKEMRRCSSEQMPCPHPAEVPEVRSK
SFDYGNLSHAPVSGAAASTVSPSRERKKCFLVRQASFSGSPEISQ"
misc_feature 2414..2419
/label=SDS
misc_feature 2550..2555
/label=SDS
misc_feature 3125..3130
/label=SDS
intron 4028..4068
/label=Splicing Donor
misc_feature 4072..4105
/label=lox2272-JT1571
repeat_region 4150..4290
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4150..4279
/label=AAV2 ITR
rep_origin 4365..4820
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5102..5206
/gene="bla"
/label=AmpR promoter
CDS 5207..6067
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6238..6826
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctga cactggggac acagctctag gacaaaaagc
781 tacctcaagg tctggagaaa ctgataaagc atcaggtaga tggagacagg aacaatcagc
841 tgttattaag atgagcactt ttggcagtca tgaaggacag cggcaaccac aaatagagcc
901 tgagcaaatc ggaaacacag catcagcaca actgtttggt tctgggaaac tggcctcccc
961 tagtgaagtg gtgcagcaag tcgcagagaa gcaatatcca ccgcatcgtc cgagtcctta
1021 ctcatgccaa cactcactct ctttccctca gcactcattg ccacaggggg tcatgcacag
1081 caccaagcca catcagagcc tcgaaggtcc tccgtggctt ttccctggcc ctttgccatc
1141 cgttgcctct gaggacttat ttccttttcc tatacatggc cacagtggtg gttatcctag
1201 aaaaaagatt tcaagtctga accctgctta tagccaatac tcccagaaaa gtattgaaca
1261 ggcagaagag gctcacaaga aagagcacaa acccaaaaag cctggcaagt acatttgccc
1321 ttactgcagc agagcgtgtg ccaaacctag tgtactgaaa aaacacatca ggtcccatac
1381 tggggagcgg ccatatccat gtataccttg tggtttctct ttcaagacaa agagcaattt
1441 gtacaagcac aggaagtcac atgcccatgc aattaaggca ggattagtac ctttcacaga
1501 gtcagctgta tctaaattgg acctagaggc tggttttatt gatgtagaag cagaaataca
1561 ttcagatggt gaacagagta cagacacaga tgaggagagt tctttatttg ccgaggcttc
1621 tgacaaaatg agtcctggtc cacccatccc actggacatt gccagcagag gcggctatca
1681 tgggtcattg gaagaatcat tgggaggtcc aatgaaggtg ccgattttga ttatccctaa
1741 aagtgggatt cctctcccta atgaaagctc tcagtatatt ggccctgata tgctaccaaa
1801 tccatcttta aatactaagg ctgatgattc gcacacagtc aaacagaaac ttgcactaag
1861 actgtcagag aaaaaaggac aagattctga gccatcgctc aaccttctga gcccgcacag
1921 taaaggaagc actgattctg gttacttttc tcgctcagaa agtgctgagc agcaaataag
1981 ccctcccaac acaaatgcaa agtcttatga agaaatcatc tttggaaaat actgtcggct
2041 tagtccgaga aatgcactca gtgttacaac cacaagtcag gagcgtgccg caatgggtag
2101 gaagggcata atggaaccat tacctcacgt taacaccagg ttagatgtca agatgtttga
2161 agatcctgtt tcacagctga tcccaagcaa gggagatgtc gaccccagtc aaacgagcat
2221 gctgaaatcc actaagttca acagtgagtc cagacaaccc cagattattc catcatctat
2281 caggaacgaa ggaaaacttt atccagcaaa cttccaaggc agcaacccgg ttctcttaga
2341 agctcctgta gactcttcac cccttattag aagcaactca gtgccaactt cttcagcaac
2401 taatctaact attcctcctt ctttgagagg aagtcactca tttgatgaaa ggatgactgg
2461 ttccgacgat gtattctatc cagggaccgt gggcataccc cctcagcgca tgctaagaag
2521 acaagcggca tttgagctgc cttcggtaca ggagggccac gtggaagtcg agcaccatgg
2581 caggatgttg aagggtatca gcagttcatc cctgaaggaa aagaaattgt ctcctgggga
2641 cagggttggg tatgactatg atgtctgtcg gaaaccctat aagaagtggg aggactctga
2701 aacaccaaag caaaactaca gggacatttc ctgcttgagt tctttaaagc atggtggaga
2761 atatttcatg gatcccgtgg tgccattgca gggagtacca agcatgtttg gaactacctg
2821 tgaaaacagg aaacgccgga aagagaagag cgtaggggat gaagaggaca cgcccatgat
2881 ctgcagcagc attgtaagca ctcctgtggg catcatggct tccgattatg accccaaact
2941 gcagatgcag gaaggagtca ggagtggatt tgccatggct ggacacgaaa acctttctca
3001 tggtcacacg gaacgctttg acccatgtcg gccccaactg cagcctggaa gtccatctct
3061 tgtgtcagag gagtcacctt cagccattga ttcagacaag atgtcagacc tagggggcag
3121 gaaacctcct ggaaatgtga tttctgtgat tcagcacacc aactcactga gccgacccaa
3181 ttcatttgaa aggtctgagt cagccgaact tgtggcttgc acacaggata aagccccttc
3241 cccttcagag acttgtgaca gtgagatttc agaagcccca gtgagtcctg agtgggctcc
3301 acctggggat ggtgcagaaa gtggggggaa accctctcca tctcagcagg tgcagcagca
3361 gtcctatcac acacagccca ggctagttcg gcaacacaac atccaggttc ctgagattcg
3421 agtgaccgag gagcctgata aacctgagaa ggagaaggaa gcccagagca aagagccaga
3481 gaagcctgtg gaagaatttc agtggcccca gagaagtgag accctttccc agctccccgc
3541 ggagaagttg ccacccaaaa agaagcgtct gcgacttgca gatatggagc actcctcagg
3601 ggagtccagc tttgaatcca caggcacagg cctctcccgc agccccagcc aagaaagcaa
3661 cttgtcccac agctccagtt tctccatgtc ttttgaaaga gaagaaacca gtaagctttc
3721 tgcacttcct aagcaggatg agtttgggaa gcattcagag tttctgactg tccctgctgg
3781 ttcatactca ttgtctgtcc caggccatca ccaccagaaa gagatgcgac gctgctcatc
3841 agagcagatg ccttgtcctc acccagcgga agtcccagaa gttcggagca aatcatttga
3901 ttatgggaat ctgtcccatg ctcctgtgtc gggagcagca gcctccacgg tatcaccgtc
3961 cagggagagg aagaaatgct ttctggtgcg gcaagcttcc ttcagtggct ccccagaaat
4021 ctcccaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
4081 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
4141 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
4201 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
4261 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
4321 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4441 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4501 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4561 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4621 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4681 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4741 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
4801 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
4861 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
4921 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
4981 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5041 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
5101 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
5161 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
5221 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
5281 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
5341 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5401 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5461 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5521 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5581 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5641 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5701 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
5761 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
5821 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
5881 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
5941 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6001 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6061 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
6121 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
6181 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6241 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6301 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6361 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6421 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6481 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6541 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6601 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6661 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6721 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6781 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6841 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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