| 质粒名称: | CDA1-BE3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 100804 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3402 bp |
| 完整质粒大小: | 8469 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CDA1-BE3 |
| 物种来源: | 合成；化脓链球菌 |
| 插入大小: | 5067 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 吉布森克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | CDA1-BE3 was a gift from David Liu (Addgene plasmid # 100804 ; http://n2t.net/addgene:100804 ; RRID:Addgene_100804) |
| 参考文献部分: | Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. aao4774 [pii] PubMed 28875174 |
买家导航
CDA1-BE3载体 CDA1BE3质粒基本信息
CDA1-BE3质粒图谱 CDA1BE3载体图谱和CDA1-BE3载体序列 CDA1BE3质粒序列多克隆位点信息
CDA1-BE3质粒 CDA1BE3载体简介
CDA1-BE3质粒序列 CDA1BE3载体序列
LOCUS CDA1-BE3 8469 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses CDA1-BE3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS CDA1-BE3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8469)
AUTHORS Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW,
Kim YB, Badran AH, Liu DR
TITLE Improved base excision repair inhibition and bacteriophage Mu Gam
protein yields C:G-to-T:A base editors with higher efficiency and
product purity.
JOURNAL Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774.
eCollection 2017 Aug.
PUBMED 28875174
REFERENCE 2 (bases 1 to 8469)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8469
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 409..1032
/codon_start=1
/product="cytidine deaminase from the sea lamprey
Petromyzon marinus (Rogozin et al., 2007)"
/label=PmCDA1
/translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
CDS 1081..5181
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5194..5442
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5455..5475
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5484..5501
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5524..5541)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5530..5754
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5825..5841)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5825..5841)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5838..5860)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5849..5865
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5873..5903)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5918..5939
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6056..6073)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6227..6815)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6307..6326)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6986..7846)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7609..7628
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7847..7951)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8030..8049)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8221..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gacagacgct
421 gaatatgtta ggatccatga aaaactggat atctatacat ttaagaagca gttcttcaat
481 aacaaaaagt cagtatctca cagatgctat gtcctgttcg aactcaagag aagaggagaa
541 aggcgggcct gtttctgggg gtacgcggtt aataaacccc agtccgggac cgagaggggg
601 attcacgccg agatcttttc aattaggaag gttgaagagt atcttcgcga caatcccggt
661 cagttcacaa ttaactggta cagctcctgg agcccttgcg ctgattgcgc cgagaaaata
721 ctcgaatggt acaaccagga gttgagaggc aatggccaca ctctcaagat ttgggcttgc
781 aagctttact acgagaagaa cgcgagaaat cagattggct tgtggaacct cagggacaac
841 ggggtcgggt tgaatgttat ggtgtccgaa cattaccagt gctgtagaaa gatcttcatt
901 cagtccagtc acaatcagct gaacgagaac agatggctgg agaaaacact gaaacgggca
961 gagaaaaggc gctcagagct gagtatcatg atccaggtca aaatcctgca tacaaccaaa
1021 agcccggctg taagcggcag cgagactccc gggacctcag agtccgccac acccgaaagt
1081 gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg ggctgtcata
1141 accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat
1201 tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac ggcagaggcg
1261 actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg aatatgttac
1321 ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt tcaccgtttg
1381 gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat ctttggaaac
1441 atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct cagaaaaaag
1501 ctagttgact caactgataa agcggacctg aggttaatct acttggctct tgcccatatg
1561 ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa ctcggatgtc
1621 gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga gaaccctata
1681 aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa atcccgacgg
1741 ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt cggtaacctt
1801 atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt agctgaagat
1861 gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct actggcacaa
1921 attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga tgcaatcctc
1981 ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc cgcttcaatg
2041 atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct agtccgtcag
2101 caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg gtacgcaggt
2161 tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc catattagag
2221 aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct actgcgaaag
2281 cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga attgcatgct
2341 atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga aaagattgag
2401 aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg gaactctcgg
2461 ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt tgaggaagtt
2521 gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt tgacaagaat
2581 ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt cacagtgtac
2641 aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc ctttctaagc
2701 ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa agtgacagtt
2761 aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt cgagatctcc
2821 ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct aaagataatt
2881 aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga tatagtgttg
2941 actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac atacgctcac
3001 ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg ctggggacga
3061 ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac tattctcgat
3121 tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca tgatgactct
3181 ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga ctcattgcac
3241 gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact ccagacagtc
3301 aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa cattgtaatc
3361 gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg agagcggatg
3421 aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga gcatcctgtg
3481 gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa tggaagggac
3541 atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt cgatcacatt
3601 gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac acgctcggat
3661 aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa aatgaagaac
3721 tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga taacttaact
3781 aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa acgtcagctc
3841 gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg aatgaatacg
3901 aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa
3961 ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat aaataactac
4021 caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat taagaaatac
4081 ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt ccgtaagatg
4141 atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt ttattctaac
4201 attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg caaacgacct
4261 ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg ggacttcgcg
4321 acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac tgaggtgcag
4381 accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa gctcatcgct
4441 cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac agttgcctat
4501 tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa gtcagtcaaa
4561 gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc catcgacttc
4621 cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact accaaagtat
4681 agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg agagcttcaa
4741 aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt agcgtcccat
4801 tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt tgttgagcag
4861 cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa gagagtcatc
4921 ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag ggataaaccc
4981 atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct cggcgctcca
5041 gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc taccaaggag
5101 gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac tcggatagat
5161 ttgtcacagc ttgggggtga ctctggtggt tctactaatc tgtcagatat tattgaaaag
5221 gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa
5281 gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc
5341 accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg
5401 gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag
5461 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
5521 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
5581 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
5641 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
5701 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
5761 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat
5821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
5881 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
5941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
6001 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
6061 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
6121 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
6181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
6241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
6301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
6661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
6721 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
6781 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
6841 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
6901 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
6961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
7021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
7081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
7141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
7201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
7261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
7321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
7381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
7441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
7501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
7561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
7621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
7681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
7741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
7801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
7861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
7921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
7981 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
8041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
8101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
8161 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
8221 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
8281 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
8341 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
8401 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
8461 aagtgtatc
//
