pLP/VSVG

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pLP/VSVG, pLP-VSVG, pLP VSVG
质粒类型: 哺乳动物细胞慢病毒包装载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5821 bp
5' 测序引物及序列: CMV-F:  CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
备注:
载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组几率。pLP/VSVG, pLP1, pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。

第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG(或者pCMV-VSVG)。在使用的时候,按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时,对于60mm的细胞培养皿来说,按照慢病毒基因质粒(3ug)和package mix(6 ug,也就是6 ul)的比例进行慢病毒质粒包装实验。
 
产品目录号: K4970-00
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLP/VSVG载体图谱


pLP/VSVG载体特征

载体简介

载体序列

LOCUS       pLP/VSVG           5821 bp ds-DNA    circular SYN 10-一月-2013
DEFINITION  Lentiviral packaging plasmid for expression of the vesicular 
            stomatitis virus G glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    pLP VSVG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        79..458
                     /label="CMV enhancer"
                     /label="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /label="CMV promoter"
                     /label="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          796..1271
                     /label="beta-globin intron"
                     /label="internally truncated intron from human?beta-globin"
     CDS             1346..2881
                     /codon_start=1
                     /gene="
                     "
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label="VSV-G"
                     /label="Indiana strain"
                     /protein_id="
                     "
     polyA_site      3082..3476
                     /label="beta-globin poly(A)"
                     /label="human?beta-globin polyadenylation signal"
     rep_origin      complement(3986..4574)
                     /direction=LEFT
                     /label="ori"
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4745..5605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="AmpR"
                     /label="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(5606..5710)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
       61 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      721 gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc gagctcggat
      781 cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta
      841 tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca
      901 gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt
      961 tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta
     1021 tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag
     1081 caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat
     1141 attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag
     1201 gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct
     1261 tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag
     1321 cacgtgagat ctgaattctg acactatgaa gtgccttttg tacttagcct ttttattcat
     1381 tggggtgaat tgcaagttca ccatagtttt tccacacaac caaaaaggaa actggaaaaa
     1441 tgttccttct aattaccatt attgcccgtc aagctcagat ttaaattggc ataatgactt
     1501 aataggcaca gccttacaag tcaaaatgcc caagagtcac aaggctattc aagcagacgg
     1561 ttggatgtgt catgcttcca aatgggtcac tacttgtgat ttccgctggt atggaccgaa
     1621 gtatataaca cattccatcc gatccttcac tccatctgta gaacaatgca aggaaagcat
     1681 tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc cctcctcaaa gttgtggata
     1741 tgcaactgtg acggatgccg aagcagtgat tgtccaggtg actcctcacc atgtgctggt
     1801 tgatgaatac acaggagaat gggttgattc acagttcatc aacggaaaat gcagcaatta
     1861 catatgcccc actgtccata actctacaac ctggcattct gactataagg tcaaagggct
     1921 atgtgattct aacctcattt ccatggacat caccttcttc tcagaggacg gagagctatc
     1981 atccctggga aaggagggca cagggttcag aagtaactac tttgcttatg aaactggagg
     2041 caaggcctgc aaaatgcaat actgcaagca ttggggagtc agactcccat caggtgtctg
     2101 gttcgagatg gctgataagg atctctttgc tgcagccaga ttccctgaat gcccagaagg
     2161 gtcaagtatc tctgctccat ctcagacctc agtggatgta agtctaattc aggacgttga
     2221 gaggatcttg gattattccc tctgccaaga aacctggagc aaaatcagag cgggtcttcc
     2281 aatctctcca gtggatctca gctatcttgc tcctaaaaac ccaggaaccg gtcctgcttt
     2341 caccataatc aatggtaccc taaaatactt tgagaccaga tacatcagag tcgatattgc
     2401 tgctccaatc ctctcaagaa tggtcggaat gatcagtgga actaccacag aaagggaact
     2461 gtgggatgac tgggcaccat atgaagacgt ggaaattgga cccaatggag ttctgaggac
     2521 cagttcagga tataagtttc ctttatacat gattggacat ggtatgttgg actccgatct
     2581 tcatcttagc tcaaaggctc aggtgttcga acatcctcac attcaagacg ctgcttcgca
     2641 acttcctgat gatgagagtt tattttttgg tgatactggg ctatccaaaa atccaatcga
     2701 gcttgtagaa ggttggttca gtagttggaa aagctctatt gcctcttttt tctttatcat
     2761 agggttaatc attggactat tcttggttct ccgagttggt atccatcttt gcattaaatt
     2821 aaagcacacc aagaaaagac agatttatac agacatagag atgaaccgac ttggaaagta
     2881 actcaaatcc tgcacaacag attcttcatg tttggaccaa atcaacttgt gataccatgc
     2941 tcaaagaggc ctcaattata tttgagtttt taatttttat gaaaaaaaaa aaaaaaaacg
     3001 gaattcaccc caccagtgca ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc
     3061 ctggcccaca agtatcacta agctcgcttt cttgctgtcc aatttctatt aaaggttcct
     3121 ttgttcccta agtccaacta ctaaactggg ggatattatg aagggccttg agcatctgga
     3181 ttctgcctaa taaaaaacat ttattttcat tgcaatgatg tatttaaatt atttctgaat
     3241 attttactaa aaagggaatg tgggaggtca gtgcatttaa aacataaaga aatgaagagc
     3301 tagttcaaac cttgggaaaa tacactatat cttaaactcc atgaaagaag gtgaggctgc
     3361 aaacagctaa tgcacattgg caacagcccc tgatgcctat gccttattca tccctcagaa
     3421 aaggattcaa gtagaggctt gatttggagg ttaaagtttt gctatgctgt attttacatt
     3481 acttattgtt ttagctgtcc tcatgaatgt cttttcacta cccatttgct tatcctgcat
     3541 ctctcagcct tgactccact cagttctctt gcttagagat accacctttc ccctgaagtg
     3601 ttccttccat gttttacggc gagatggttt ctcctcgcct ggccactcag ccttagttgt
     3661 ctctgttgtc ttatagaggt ctacttgaag aaggaaaaac agggggcatg gtttgactgt
     3721 cctgtgagcc cttcttccct gcctccccca ctcacagtga cccggaatcc ctcgacatgg
     3781 cagtctagca ctagtgcggc cgcagatctg cttcctcgct cactgactcg ctgcgctcgg
     3841 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3901 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3961 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4021 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4081 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4141 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4201 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4261 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4321 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4381 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4441 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4501 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4561 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4621 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4681 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4741 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4801 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4861 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4921 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4981 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5041 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5101 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5161 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5221 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5281 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5341 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5401 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5461 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5521 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5581 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5641 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5701 gggttccgcg cacatttccc cgaaaagtgc cacctgacgg gatcccctga gggggccccc
     5761 atgggctaga ggatccggcc tcggcctctg cataaataaa aaaaattagt cagccatgag
     5821 c
//

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