| 质粒名称: | pEP4 E02S EM2K |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20923 |
| 存储实验室: | James Thomson |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 12600 bp |
| 载体类型: | 游离型/ EBNA |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Oct4 and Sox2; Myc and Klf4 |
| 物种来源: | H. sapiens (人) |
| 插入大小: | 2700 bp |
| GenBank编号 | NM_002701 NM_004235 NM_002467 NM_003106 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | multiple(未知是否被破坏) |
| 3' 克隆位点: | multiple(未知是否被破坏) |
| 5' 测序引物 | na |
|
文献引用方法 |
|
| 材料和方法部分: | pEP4 E02S EM2K was a gift from James Thomson (Addgene plasmid # 20923) |
| 参考文献部分: |
Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEP4 E02S EM2K Sequencing Result 15885 bp DNA SYN
DEFINITION pEP4 E02S EM2K Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..15885
/organism="pEP4 E02S EM2K Sequencing Result"
/mol_type="other DNA"
misc_feature 263..847
/label="IRES"
CDS 747..1823
/label="ORF frame 3"
terminator complement(1866..1985)
/label="SV40_PA_terminator"
misc_feature complement(1880..1899)
/label="EBV_rev_primer"
misc_feature complement(2133..2155)
/label="pGEX_3_primer"
CDS complement(2511..3425)
/label="ORF frame 2"
promoter 2518..3685
/label="EF1a_promoter"
misc_feature 3633..3653
/label="EF1a_fwd_primer"
CDS 3817..5136
/label="ORF frame 1"
misc_feature 5044..5073
/label="Myc"
misc_feature 5202..5786
/label="IRES"
CDS 5686..7224
/label="ORF frame 1"
CDS complement(6169..7020)
/label="ORF frame 1"
terminator complement(7273..7392)
/label="SV40_PA_terminator"
misc_feature complement(7287..7306)
/label="EBV_rev_primer"
misc_feature complement(7540..7562)
/label="pGEX_3_primer"
rep_origin complement(7751..9734)
/label="EBV_origin"
CDS complement(10045..11346)
/label="ORF frame 1"
CDS complement(10743..11231)
/label="ORF frame 2"
CDS 10758..11348
/label="ORF frame 3"
promoter 11906..11934
/label="AmpR_promoter"
gene 11976..12836
/label="Ampicillin"
/gene="Ampicillin"
CDS 11976..12836
/label="ORF frame 3"
rep_origin 12991..13610
/label="pBR322_origin"
CDS complement(13765..14679)
/label="ORF frame 1"
promoter 13772..14939
/label="EF1a_promoter"
misc_feature 14887..14907
/label="EF1a_fwd_primer"
CDS 14993..190
/label="ORF frame 2"
ORIGIN
1 TGAGGCTGCT GGGTCTCCTT TCTCAGGGGG ACCAGTGTCC TTTCCTCTGG CCCCAGGGCC
61 CCATTTTGGT ACCCCAGGCT ATGGGAGCCC TCACTTCACT GCACTGTACT CCTCGGTCCC
121 TTTCCCTGAG GGGGAAGCCT TTCCCCCTGT CTCCGTCACC ACTCTGGGCT CTCCCATGCA
181 TTCAAACTGA GGTGCCTGCC CTCTAGTTTA CGCGTGGGGG CGGCCGGCCG CTTTCTCGAG
241 GCCATCGATG TTTAAACGAT CCGCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA
301 AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG
361 TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG
421 GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT
481 CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC
541 CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA
601 AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG
661 CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG
721 GGATCTGATC TGGGGCCTCG GTACACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA
781 CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT
841 GGCCACACCG CGAATTAATT CGATTCCGCA TGTACAACAT GATGGAGACG GAGCTGAAGC
901 CGCCGGGCCC GCAGCAAACT TCGGGGGGCG GCGGCGGCAA CTCCACCGCG GCGGCGGCCG
961 GCGGCAACCA GAAAAACAGC CCGGACCGCG TCAAGCGGCC CATGAATGCC TTCATGGTGT
1021 GGTCCCGCGG GCAGCGGCGC AAGATGGCCC AGGAGAACCC CAAGATGCAC AACTCGGAGA
1081 TCAGCAAGCG CCTGGGCGCC GAGTGGAAAC TTTTGTCGGA GACGGAGAAG CGGCCGTTCA
1141 TCGACGAGGC TAAGCGGCTG CGAGCGCTGC ACATGAAGGA GCACCCGGAT TATAAATACC
1201 GGCCCCGGCG GAAAACCAAG ACGCTCATGA AGAAGGATAA GTACACGCTG CCCGGCGGGC
1261 TGCTGGCCCC CGGCGGCAAT AGCATGGCGA GCGGGGTCGG GGTGGGCGCC GGCCTGGGCG
1321 CGGGCGTGAA CCAGCGCATG GACAGTTACG CGCACATGAA CGGCTGGAGC AACGGCAGCT
1381 ACAGCATGAT GCAGGACCAG CTGGGCTACC CGCAGCACCC GGGCCTCAAT GCGCACGGCG
1441 CAGCGCAGAT GCAGCCCATG CACCGCTACG ACGTGAGCGC CCTGCAGTAC AACTCCATGA
1501 CCAGCTCGCA GACCTACATG AACGGCTCGC CCACCTACAG CATGTCCTAC TCGCAGCAGG
1561 GCACCCCTGG CATGGCTCTT GGCTCCATGG GTTCGGTGGT CAAGTCCGAG GCCAGCTCCA
1621 GCCCCCCTGT GGTTACCTCT TCCTCCCACT CCAGGGCGCC CTGCCAGGCC GGGGACCTCC
1681 GGGACATGAT CAGCATGTAT CTCCCCGGCG CCGAGGTGCC GGAACCCGCC GCCCCCAGCA
1741 GACTTCACAT GTCCCAGCAC TACCAGAGCG GCCCGGTGCC CGGCACGGCC ATTAACGGCA
1801 CACTGCCCCT CTCACACATG TGAGGGCCGG AAATCACTAG CTAGTTCGAA GGATCGATCC
1861 AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
1921 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
1981 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG
2041 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCCGGC
2101 TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG
2161 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG
2221 GGTGTTGGCG GGTGTCGGGG CGCAGCCATG AGGTCGACCA ATTCTCATGT TTGACAGCTT
2281 ATCATCGCAG ATCCGGGCAA CGTTGTTGCC ATTGCTGCAG GCGCAGAACT GGTAGGTATG
2341 GAAGATCTAT ACATTGAATC AATATTGGCA ATTAGCCATA TTAGTCATTG GTTATATAGC
2401 ATAAATCAAT ATTGGCTATT GGCCATTGCA TACGTTGTAT CTATATCATA ATATGTACAT
2461 TTATATTGGC TCATGTCCAA TATGACCGCC ATGTTGACAT TGATTATTGA CTAGCTTCGT
2521 GAGGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG
2581 GGGGAGGGGT CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA
2641 GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG
2701 CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG
2761 CCGTGTGTGG TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA
2821 TTACTTCCAC CTGGCTCCAG TACGTGATTC TTGATCCCGA GCTGGAGCCA GGGGCGGGCC
2881 TTGCGCTTTA GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG
2941 CCGCCGCGTG CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT
3001 AGCCATTTAA AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT
3061 AAATGCGGGC CAGGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG
3121 GGCCCGTGCG TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG
3181 AATCGGACGG GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC
3241 GTGTATCGCC CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA
3301 AAGATGGCCG CTTCCCGGCC CTGCTCCAGG GGGCTCAAAA TGGAGGACGC GGCGCTCGGG
3361 AGAGCGGGCG GGTGAGTCAC CCACACAAAG GAAAGGGGCC TTTCCGTCCT CAGCCGTCGC
3421 TTCATGTGAC TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTGGAGCTT
3481 TTGGAGTACG TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC
3541 TGAGTGGGTG GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
3601 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT
3661 TTTTCTTCCA TTTCAGGTGT CGTGAACACG TGGTCGCGGC CAATTCTGCA GTCGAGCTCA
3721 AGCTTCGAAC TAGGGCCGCG ACTCTAGAAG CTGGGTACCA GCTGCTAGCG CTACCGGACT
3781 CAGATCTCGA GCTCAAGCTT CGAATTCGCG ATCGCCATGC CCCTCAACGT TAGCTTCACC
3841 AACAGGAACT ATGACCTCGA CTACGACTCG GTGCAGCCGT ATTTCTACTG CGACGAGGAG
3901 GAGAACTTCT ACCAGCAGCA GCAGCAGAGC GAGCTGCAGC CCCCGGCGCC CAGCGAGGAT
3961 ATCTGGAAGA AATTCGAGCT GCTGCCCACC CCGCCCCTGT CCCCTAGCCG CCGCTCCGGG
4021 CTCTGCTCGC CCTCCTACGT TGCGGTCACA CCCTTCTCCC TTCGGGGAGA CAACGACGGC
4081 GGTGGCGGGA GCTTCTCCAC GGCCGACCAG CTGGAGATGG TGACCGAGCT GCTGGGAGGA
4141 GACATGGTGA ACCAGAGTTT CATCTGCGAC CCGGACGACG AGACCTTCAT CAAAAACATC
4201 ATCATCCAGG ACTGTATGTG GAGCGGCTTC TCGGCCGCCG CCAAGCTCGT CTCAGAGAAG
4261 CTGGCCTCCT ACCAGGCTGC GCGCAAAGAC AGCGGCAGCC CGAACCCCGC CCGCGGCCAC
4321 AGCGTCTGCT CCACCTCCAG CTTGTACCTG CAGGATCTGA GCGCCGCCGC CTCAGAGTGC
4381 ATCGACCCCT CGGTGGTCTT CCCCTACCCT CTCAACGACA GCAGCTCGCC CAAGTCCTGC
4441 GCCTCGCAAG ACTCCAGCGC CTTCTCTCCG TCCTCGGATT CTCTGCTCTC CTCGACGGAG
4501 TCCTCCCCGC AGGGCAGCCC CGAGCCCCTG GTGCTCCATG AGGAGACACC GCCCACCACC
4561 AGCAGCGACT CTGAGGAGGA ACAAGAAGAT GAGGAAGAAA TCGATGTTGT TTCTGTGGAA
4621 AAGAGGCAGG CTCCTGGCAA AAGGTCAGAG TCTGGATCAC CTTCTGCTGG AGGCCACAGC
4681 AAACCTCCTC ACAGCCCACT GGTCCTCAAG AGGTGCCACG TCTCCACACA TCAGCACAAC
4741 TACGCAGCGC CTCCCTCCAC TCGGAAGGAC TATCCTGCTG CCAAGAGGGT CAAGTTGGAC
4801 AGTGTCAGAG TCCTGAGACA GATCAGCAAC AACCGAAAAT GCACCAGCCC CAGGTCCTCG
4861 GACACCGAGG AGAATGTCAA GAGGCGAACA CACAACGTCT TGGAGCGCCA GAGGAGGAAC
4921 GAGCTAAAAC GGAGCTTTTT TGCCCTGCGT GACCAGATCC CGGAGTTGGA AAACAATGAA
4981 AAGGCCCCCA AGGTAGTTAT CCTTAAAAAA GCCACAGCAT ACATCCTGTC CGTCCAAGCA
5041 GAGGAGCAAA AGCTCATTTC TGAAGAGGAC TTGTTGCGGA AACGACGAGA ACAGTTGAAA
5101 CACAAACTTG AACAGCTACG GAACTCTTGT GCGTAAGTTT AAACATCGAT GGCCTCGAGA
5161 AAGCGGCCGC TTTCTCGAGG CCATCGATGT TTAAACGATC CGCCCCTCTC CCTCCCCCCC
5221 CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT
5281 ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT
5341 CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA
5401 TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC
5461 CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG
5521 TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT
5581 TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA
5641 GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TACACATGCT TTACATGTGT
5701 TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG
5761 AAAAACACGA TGATAATATG GCCACACCGC GATCGCGAAT TCGCGATCGC CATGGCTGTC
5821 AGCGACGCGC TGCTCCCATC TTTCTCCACG TTCGCGTCTG GCCCGGCGGG AAGGGAGAAG
5881 ACACTGCGTC AAGCAGGTGC CCCGAATAAC CGCTGGCGGG AGGAGCTCTC CCACATGAAG
5941 CGACTTCCCC CAGTGCTTCC CGGCCGCCCC TATGACCTGG CGGCGGCGAC CGTGGCCACA
6001 GACCTGGAGA GCGGCGGAGC CGGTGCGGCT TGCGGCGGTA GCAACCTGGC GCCCCTACCT
6061 CGGAGAGAGA CCGAGGAGTT CAACGATCTC CTGGACCTGG ACTTTATTCT CTCCAATTCG
6121 CTGACCCATC CTCCGGAGTC AGTGGCCGCC ACCGTGTCCT CGTCAGCGTC AGCCTCCTCT
6181 TCGTCGTCGC CGTCGAGCAG CGGCCCTGCC AGCGCGCCCT CCACCTGCAG CTTCACCTAT
6241 CCGATCCGGG CCGGGAACGA CCCGGGCGTG GCGCCGGGCG GCACGGGCGG AGGCCTCCTC
6301 TATGGCAGGG AGTCCGCTCC CCCTCCGACG GCTCCCTTCA ACCTGGCGGA CATCAACGAC
6361 GTGAGCCCCT CGGGCGGCTT CGTGGCCGAG CTCCTGCGGC CAGAATTGGA CCCGGTGTAC
6421 ATTCCGCCGC AGCAGCCGCA GCCGCCAGGT GGCGGGCTGA TGGGCAAGTT CGTGCTGAAG
6481 GCGTCGCTGA GCGCCCCTGG CAGCGAGTAC GGCAGCCCGT CGGTCATCAG CGTCAGCAAA
6541 GGCAGCCCTG ACGGCAGCCA CCCGGTGGTG GTGGCGCCCT ACAACGGCGG GCCGCCGCGC
6601 ACGTGCCCCA AGATCACGCA GGAGGCGGTC TCTTCGTGCA CCCACTTGGG CGCTGGACCC
6661 CCTCTCAGCA ATGGCCACCG GCCGGCTGCA CACGACTTCC CCCTGGGGCG GCAGCTCCCC
6721 AGCAGGACTA CCCCGACCCT GGGTCTTGAG GAAGTGCTGA GCAGCAGGGA CTGTCACCCT
6781 GCCCTGCCGC TTCCTCCCGG CTTCCATCCC CACCCGGGGC CCAATTACCC ATCCTTCCTG
6841 CCCGATCAGA TGCAGCCGCA AGTCCCGCCG CTCCATTACC AAGAGCTCAT GCCACCCGGT
6901 TCCTGCATGC CAGAGGAGCC CAAGCCAAAG AGGGGAAGAC GATCGTGGCC CCGGAAAAGG
6961 ACCGCCACCC ACACTTGTGA TTACGCGGGC TGCGGCAAAA CCTACACAAA GAGTTCCCAT
7021 CTCAAGGCAC ACCTGCGAAC CCACACAGGT GAGAAACCTT ACCACTGTGA CTGGGACGGC
7081 TGTGGATGGA AATTCGCCCG CTCAGATGAA CTGACCAGGC ACTACCGTAA ACACACGGGG
7141 CACCGCCCGT TCCAGTGCCA AAAATGCGAC CGAGCATTTT CCAGGTCGGA CCACCTCGCC
7201 TTACACATGA AGAGGCATTT TTAAATCCCA GACAGTGGAT ATGACCCGTT TCTAGTTCGA
7261 AGGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT
7321 GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
7381 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
7441 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
7501 ATCCGGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC
7561 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC
7621 GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGAGG TCGATCGACT CTAGCTAGAG
7681 GATCGATCCC CGCCCCGGAC GAACTAAACC TGACTACGAC ATCTCTGCCC CTTCTTCGCG
7741 GGGCAGTGCA TGTAATCCCT TCAGTTGGTT GGTACAACTT GCCAACTGGG CCCTGTTCCA
7801 CATGTGACAC GGGGGGGGAC CAAACACAAA GGGGTTCTCT GACTGTAGTT GACATCCTTA
7861 TAAATGGATG TGCACATTTG CCAACACTGA GTGGCTTTCA TCCTGGAGCA GACTTTGCAG
7921 TCTGTGGACT GCAACACAAC ATTGCCTTTA TGTGTAACTC TTGGCTGAAG CTCTTACACC
7981 AATGCTGGGG GACATGTACC TCCCAGGGGC CCAGGAAGAC TACGGGAGGC TACACCAACG
8041 TCAATCAGAG GGGCCTGTGT AGCTACCGAT AAGCGGACCC TCAAGAGGGC ATTAGCAATA
8101 GTGTTTATAA GGCCCCCTTG TTAACCCTAA ACGGGTAGCA TATGCTTCCC GGGTAGTAGT
8161 ATATACTATC CAGACTAACC CTAATTCAAT AGCATATGTT ACCCAACGGG AAGCATATGC
8221 TATCGAATTA GGGTTAGTAA AAGGGTCCTA AGGAACAGCG ATATCTCCCA CCCCATGAGC
8281 TGTCACGGTT TTATTTACAT GGGGTCAGGA TTCCACGAGG GTAGTGAACC ATTTTAGTCA
8341 CAAGGGCAGT GGCTGAAGAT CAAGGAGCGG GCAGTGAACT CTCCTGAATC TTCGCCTGCT
8401 TCTTCATTCT CCTTCGTTTA GCTAATAGAA TAACTGCTGA GTTGTGAACA GTAAGGTGTA
8461 TGTGAGGTGC TCGAAAACAA GGTTTCAGGT GACGCCCCCA GAATAAAATT TGGACGGGGG
8521 GTTCAGTGGT GGCATTGTGC TATGACACCA ATATAACCCT CACAAACCCC TTGGGCAATA
8581 AATACTAGTG TAGGAATGAA ACATTCTGAA TATCTTTAAC AATAGAAATC CATGGGGTGG
8641 GGACAAGCCG TAAAGACTGG ATGTCCATCT CACACGAATT TATGGCTATG GGCAACACAT
8701 AATCCTAGTG CAATATGATA CTGGGGTTAT TAAGATGTGT CCCAGGCAGG GACCAAGACA
8761 GGTGAACCAT GTTGTTACAC TCTATTTGTA ACAAGGGGAA AGAGAGTGGA CGCCGACAGC
8821 AGCGGACTCC ACTGGTTGTC TCTAACACCC CCGAAAATTA AACGGGGCTC CACGCCAATG
8881 GGGCCCATAA ACAAAGACAA GTGGCCACTC TTTTTTTTGA AATTGTGGAG TGGGGGCACG
8941 CGTCAGCCCC CACACGCCGC CCTGCGGTTT TGGACTGTAA AATAAGGGTG TAATAACTTG
9001 GCTGATTGTA ACCCCGCTAA CCACTGCGGT CAAACCACTT GCCCACAAAA CCACTAATGG
9061 CACCCCGGGG AATACCTGCA TAAGTAGGTG GGCGGGCCAA GATAGGGGCG CGATTGCTGC
9121 GATCTGGAGG ACAAATTACA CACACTTGCG CCTGAGCGCC AAGCACAGGG TTGTTGGTCC
9181 TCATATTCAC GAGGTCGCTG AGAGCACGGT GGGCTAATGT TGCCATGGGT AGCATATACT
9241 ACCCAAATAT CTGGATAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TAGGCTATCC
9301 TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC
9361 TAATTTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC
9421 TAATCTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC
9481 TAATCTATAT CTGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TAGGCTATCC
9541 TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC
9601 TAATCTGTAT CCGGGTAGCA TATGCTATCC TCATGCATAT ACAGTCAGCA TATGATACCC
9661 AGTAGTAGAG TGGGAGTGCT ATCCTTTGCA TATGCCGCCA CCTCCCAAGG GGGCGTGAAT
9721 TTTCGCTGCT TGTCCTTTTC CTGCTGGTTG CTCCCATTCT TAGGTGAATT TAAGGAGGCC
9781 AGGCTAAAGC CGTCGCATGT CTGATTGCTC ACCAGGTAAA TGTCGCTAAT GTTTTCCAAC
9841 GCGAGAAGGT GTTGAGCGCG GAGCTGAGTG ACGTGACAAC ATGGGTATGC CCAATTGCCC
9901 CATGTTGGGA GGACGAAAAT GGTGACAAGA CAGATGGCCA GAAATACACC AACAGCACGC
9961 ATGATGTCTA CTGGGGATTT ATTCTTTAGT GCGGGGGAAT ACACGGCTTT TAATACGATT
10021 GAGGGCGTCT CCTAACAAGT TACATCACTC CTGCCCTTCC TCACCCTCAT CTCCATCACC
10081 TCCTTCATCT CCGTCATCTC CGTCATCACC CTCCGCGGCA GCCCCTTCCA CCATAGGTGG
10141 AAACCAGGGA GGCAAATCTA CTCCATCGTC AAAGCTGCAC ACAGTCACCC TGATATTGCA
10201 GGTAGGAGCG GGCTTTGTCA TAACAAGGTC CTTAATCGCA TCCTTCAAAA CCTCAGCAAA
10261 TATATGAGTT TGTAAAAAGA CCATGAAATA ACAGACAATG GACTCCCTTA GCGGGCCAGG
10321 TTGTGGGCCG GGTCCAGGGG CCATTCCAAA GGGGAGACGA CTCAATGGTG TAAGACGACA
10381 TTGTGGAATA GCAAGGGCAG TTCCTCGCCT TAGGTTGTAA AGGGAGGTCT TACTACCTCC
10441 ATATACGAAC ACACCGGCGA CCCAAGTTCC TTCGTCGGTA GTCCTTTCTA CGTGACTCCT
10501 AGCCAGGAGA GCTCTTAAAC CTTCTGCAAT GTTCTCAAAT TTCGGGTTGG AACCTCCTTG
10561 ACCACGATGC TTTCCAAACC ACCCTCCTTT TTTGCGCCTG CCTCCATCAC CCTGACCCCG
10621 GGGTCCAGTG CTTGGGCCTT CTCCTGGGTC ATCTGCGGGG CCCTGCTCTA TCGCTCCCGG
10681 GGGCACGTCA GGCTCACCAT CTGGGCCACC TTCTTGGTGG TATTCAAAAT AATCGGCTTC
10741 CCCTACAGGG TGGAAAAATG GCCTTCTACC TGGAGGGGGC CTGCGCGGTG GAGACCCGGA
10801 TGATGATGAC TGACTACTGG GACTCCTGGG CCTCTTTTCT CCACGTCCAC GACCTCTCCC
10861 CCTGGCTCTT TCACGACTTC CCCCCCTGGC TCTTTCACGT CCTCTACCCC GGCGGCCTCC
10921 ACTACCTCCT CGACCCCGGC CTCCACTACC TCCTCGACCC CGGCCTCCAC TGCCTCCTCG
10981 ACCCCGGCCT CCACCTCCTG CTCCTGCCCC TCCTGCTCCT GCCCCTCCTC CTGCTCCTGC
11041 CCCTCCTGCT CCTGCCCCTC CCGCTCCTGC TCCTGCTCCT GTTCCACCGT GGGTCCCTTT
11101 GCAGCCAATG CAACTTGGAC GTTTTTGGGG TCTCCGGACA CCATCTCTAT GTCTTGGCCC
11161 TGATCCTGAG CCGCCCGGGG CTCCTGGTCT TCCGCCTCCT CGTCCTCGTC CTCTTCCCCG
11221 TCCTCGTCCA TGGTTATCAC CCCCTCTTCT TTGAGGTCCA CTGCCGCCGG AGCCTTCTGG
11281 TCCAGATGTG TCTCCCTTCT CTCCTAGGCC ATTTCCAGGT CCTGTACCTG GCCCCTCGTC
11341 AGACATGATT CACACTAAAA GAGATCAATA GACATCTTTA TTAGACGACG CTCAGTGAAT
11401 ACAGGGAGTG CAGACTCCTG CCCCCTCCAA CAGCCCCCCC ACCCTCATCC CCTTCATGGT
11461 CGCTGTCAGA CAGATCCAGG TCTGAAAATT CCCCATCCTC CGAACCATCC TCGTCCTCAT
11521 CACCAATTAC TCGCAGCCCG GAAAACTCCC GCTGAACATC CTCAAGATTT GCGTCCTGAG
11581 CCTCAAGCCA GGCCTCAAAT TCCTCGTCCC CCTTTTTGCT GGACGGTAGG GATGGGGATT
11641 CTCGGGACCC CTCCTCTTCC TCTTCAAGGT CACCAGACAG AGATGCTACT GGGGCAACGG
11701 AAGAAAAGCT GGGTGCGGCC TGTGAGGATC AGCTTATCGA TGATAAGCTG TCAAACATGA
11761 GAATTAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA
11821 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC
11881 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
11941 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG
12001 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG
12061 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC
12121 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA
12181 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC
12241 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA
12301 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG
12361 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT
12421 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG
12481 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC
12541 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA
12601 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA
12661 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC
12721 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG
12781 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT
12841 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA
12901 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT
12961 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
13021 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
13081 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
13141 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
13201 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
13261 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
13321 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
13381 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
13441 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
13501 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
13561 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
13621 GGTTCCTGGC CTTTTGCTGG CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG
13681 ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG
13741 GGAAGGCCAT CCAGCCTCGC GTCGCTAGCT TCGTGAGGCT CCGGTGCCCG TCAGTGGGCA
13801 GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACCGGT
13861 GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT
13921 TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT
13981 CGCAACGGGT TTGCCGCCAG AACACAGGTA AGTGCCGTGT GTGGTTCCCG CGGGCCTGGC
14041 CTCTTTACGG GTTATGGCCC TTGCGTGCCT TGAATTACTT CCACCTGGCT CCAGTACGTG
14101 ATTCTTGATC CCGAGCTGGA GCCAGGGGCG GGCCTTGCGC TTTAGGAGCC CCTTCGCCTC
14161 GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC
14221 TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG
14281 CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAGGAT CTGCACACTG
14341 GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT
14401 CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG
14461 GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA
14521 GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTC
14581 CAGGGGGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC
14641 AAAGGAAAGG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG
14701 CGCCGTCCAG GCACCTCGAT TAGTTCTGGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG
14761 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC
14821 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT
14881 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAA
14941 CACGTGGTCG CGGCCAATTC TGCAGTCGAG CTCAAGCTTC GAACTAGTGA TTATGGCGGG
15001 ACACCTGGCT TCGGATTTCG CCTTCTCGCC CCCTCCAGGT GGTGGAGGTG ATGGGCCAGG
15061 GGGGCCGGAG CCGGGCTGGG TTGATCCTCG GACCTGGCTA AGCTTCCAAG GCCCTCCTGG
15121 AGGGCCAGGA ATCGGGCCGG GGGTTGGGCC AGGCTCTGAG GTGTGGGGGA TTCCCCCATG
15181 CCCCCCGCCG TATGAGTTCT GTGGGGGGAT GGCGTACTGT GGGCCCCAGG TTGGAGTGGG
15241 GCTAGTGCCC CAAGGCGGCT TGGAGACCTC TCAGCCTGAG GGTGAAGCAG GAGTCGGGGT
15301 GGAGAGCAAC TCCGATGGGG CCTCCCCGGA GCCCTGCACC GTCACCCCTG GTGCCGTGAA
15361 GCTGGAGAAG GAGAAGCTGG AGCAAAACCC GGAGGAGTCC CAGGACATCA AAGCTCTGCA
15421 GAAAGAACTC GAGCAATTTG CCAAGCTCCT GAAGCAGAAG AGGATCACCC TGGGATATAC
15481 ACAGGCCGAT GTGGGGCTCA CCCTGGGGGT TCTATTTGGG AAGGTATTCA GCCAAACGAC
15541 CATCTGCCGC TTTGAGGCTC TGCAGCTTAG CTTCAAGAAC ATGTGTAAGC TGCGGCCCTT
15601 GCTGCAGAAG TGGGTGGAGG AAGCTGACAA CAATGAAAAT CTTCAGGAGA TATGCAAAGC
15661 AGAAACCCTC GTGCAGGCCC GAAAGAGAAA GCGAACCAGT ATCGAGAACC GAGTGAGAGG
15721 CAACCTGGAG AATTTGTTCC TGCAGTGCCC GAAACCCACA CTGCAGCAGA TCAGCCACAT
15781 CGCCCAGCAG CTTGGGCTCG AGAAGGATGT GGTCCGAGTG TGGTTCTGTA ACCGGCGCCA
15841 GAAGGGCAAG CGATCAAGCA GCGACTATGC ACAACGAGAG GATTT
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