| 质粒名称: | pCP5b |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37185 |
| 存储实验室: | Adam Bogdanove |
|
载体骨架基本信息 |
|
| 载体名称: | pCP5 |
| 载体出品公司: | Voytas lab (Addgene Plasmid #35397) |
| 载体抗性: | 氯霉素和氨苄青霉素 |
| 空载体大小: | 13853 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 质粒pCP5酵母2微子复制源和卡那霉素抗性基因替换为pBR322复制源和来自pBluescript SK的氨苄青霉素抗性盒（以提高复制产量） |
| 载体类型: | 酵母表达 |
| 筛选标记: | TRP1 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCP5b was a gift from Adam Bogdanove (Addgene plasmid # 37185 ; http://n2t.net/addgene:37185 ; RRID:Addgene_37185) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCP5b 12874 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCP5b
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12874)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 12874)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12874
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 21..37
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 21..37
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 97..740
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase"
primer_bind 634..657
/label=GPDpro-F
/note="S. cerevisiae GPD promoter, forward primer"
primer_bind 776..799
/label=GAL1
/note="S. cerevisiae GAL1 promoter, forward primer"
promoter 1962..2177
/gene="S. cerevisiae URA3"
/label=URA3 promoter
CDS 2178..2981
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
primer_bind 3154..3170
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3187..3418
/gene="mutant version of attP"
/label=attP1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction
(pDONR(TM)201 version)"
CDS complement(3814..4119)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind complement(3916..3935)
/label=ccdB-fwd
/note="ccdB gene, forward primer"
CDS complement(4464..5123)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 5038..5057
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(5124..5226)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(5371..5602)
/gene="mutant version of attP"
/label=attP2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction
(pDONR(TM)201 version)"
terminator 7949..8195
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(7964..7982)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
primer_bind complement(8235..8251)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(8364..8952)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8444..8463)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9123..9983)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9746..9765
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9984..10088)
/gene="bla"
/label=AmpR promoter
rep_origin 10205..11547
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind complement(11168..11215)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind 11581..11599
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(11637..11659)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 11759..11778
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 11799..12080
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
CDS 12081..12755
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
ORIGIN
1 atcaacgttg gtcattcaca caggaaacag ctatgaccat gtgacttgac tgatttcagt
61 tcagtaggtc taaatcgtac gaatcatcgc agtttatcat tatcaatact cgccatttca
121 aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag
181 ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat
241 ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag
301 cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc
361 ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac
421 aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga
481 tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc
541 ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat
601 tattccccta cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa
661 atctatttct taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa
721 acaccagaac ttagtttcga cggattctag aaaggatcct caacaaaaaa ttgttaatat
781 acctctatac tttaacgtca aggagaaaaa actataatga ctaaatctca ttcagaagaa
841 gtgattgtac ctgagttcaa ttctagcgca aaggaattac caagaccatt ggccgaaaag
901 tgcggtattc caagtcttgc caagcccaga tccggagctt ggctgttgcc cgtctcactg
961 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
1021 gattcattaa tgcagctggc acgacaggtt tcccgactta atcgccttgc agcacatccc
1081 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
1141 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
1201 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
1261 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
1321 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
1381 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
1441 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt
1501 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg
1561 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
1621 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt
1681 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt
1741 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc
1801 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta
1861 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg
1921 gtggttgaac tgcacaccga gctcaacccc cataccacct tttcaattca tcattttttt
1981 tttattcttt tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac
2041 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
2101 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca
2161 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
2221 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
2281 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
2341 tttgtttact aaaaactcat gtggacatct tgactgattt ttctatggag ggcacagtta
2401 agccgctaaa ggcattatcc gccaagtaca attttttact ctttgaagac agaaaatttg
2461 ctgacattgg taatacagtc aaattgcaat actctgcggg tgtttacaga atagcagaat
2521 gggcagacat tacgaatgca cacggtgtgg tgggtccagg tattgttagc ggtttgaagc
2581 aggcggcaga agaagtaaca aaggaaccaa ggggcctttt gatgttagca gaattgtcat
2641 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
2701 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
2761 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc
2821 aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa
2881 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
2941 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
3001 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattaccct
3061 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
3121 gcgttaatgg gtttcatggt atcagagatc tttgtaaaac gacggccagt cttaagctcg
3181 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa cacattgatg
3241 agcaatgctt ttttataatg ccaactttgt acaaaaaagc tgaacgagaa acgtaaaatg
3301 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
3361 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt
3421 cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc
3481 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
3541 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
3601 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
3661 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
3721 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
3781 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
3841 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
3901 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
3961 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
4021 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
4081 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
4141 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
4201 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
4261 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
4321 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
4381 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
4441 agtaagccgg atccacgcgg cgtttacgcc ccgccctgcc actcatcgca gtactgttgt
4501 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
4561 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
4621 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
4681 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
4741 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
4801 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
4861 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
4921 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
4981 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
5041 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
5101 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
5161 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
5221 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
5281 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
5341 gcgtcgggtg atgctgccaa cttagtcgac tacaggtcac taataccatc taagtagttg
5401 attcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
5461 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
5521 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
5581 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtaggac
5641 tagtgctgga gggtgtacca ccaatctcga gtattcagca tgctggtaag ctagcttttc
5701 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
5761 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
5821 ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
5881 ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
5941 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
6001 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
6061 acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
6121 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
6181 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
6241 aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
6301 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
6361 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
6421 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
6481 cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
6541 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
6601 gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
6661 cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
6721 gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
6781 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
6841 ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
6901 attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
6961 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag
7021 tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat
7081 ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
7141 cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg
7201 ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
7261 cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
7321 gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct
7381 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat
7441 ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg
7501 gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga
7561 ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat
7621 ctgccattgt cagacatgta taccccatac gtcttcccga gcgaaaacgg tctgcgctgc
7681 gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
7741 agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
7801 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
7861 agcccgtcag tatcggcgga atttcaactg agcgccggtc gctaccatta ccagttggtc
7921 tggtgtcaaa aataatgata gtcatgagca tgtaattagt tatgtcacgc ttacattcac
7981 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
8041 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
8101 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
8161 agaaggtttt gggacgctcg aaggctttaa tttgcgggcc ctgtcataca ttatcaacag
8221 gacttcataa gcaaactggc cgtcgtttta catcggccgg taatacggtt atccacagaa
8281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
8341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
8401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
8461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
8521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
8581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
8641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
8701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
8761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
8821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
8881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
8941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
9001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
9061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
9121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
9181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
9241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
9301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
9361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
9421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
9481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
9541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
9601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
9661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
9721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
9781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
9841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
9901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
9961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
10021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
10081 gttccgcgca ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
10141 accgacgacc gcgttcttcg gggcgaaaac tctccgcgca catttccccg aaaagtgcca
10201 cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
10261 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
10321 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
10381 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
10441 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
10501 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
10561 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
10621 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
10681 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
10741 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
10801 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
10861 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
10921 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
10981 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
11041 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
11101 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
11161 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc
11221 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
11281 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
11341 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta
11401 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc
11461 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa
11521 tgctatcatt tcctttgata ttggatcata ctaagaaacc attattaatt aacctataaa
11581 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
11641 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
11701 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
11761 gcatcagagc agattgtact gagagtgcac catagatcaa cgacattact atatatataa
11821 tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag ttatgacgcc
11881 agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt acgtacaatc
11941 ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag
12001 gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac gtgattaagc
12061 acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct ggtccattgg
12121 tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat tccgatgctg
12181 acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa
12241 ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg
12301 ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca
12361 ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt
12421 tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt
12481 cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac
12541 ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac
12601 attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg
12661 gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca
12721 aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag tatttattta
12781 agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga
12841 gaaaataccg catcaggaaa ttgtaagcgt taat
//
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