| 质粒名称: | HBT-pcoCas9,pHBT-pcoCas9-FLAG-NLS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 52254 |
| 存储实验室: | Jen Sheen |
|
载体骨架基本信息 |
|
| 载体名称: | HBT-FLAG |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | CRISPR,植物表达 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pcoCas9,plant codon–optimized Cas9 |
| 物种来源: | Synthetic |
| GenBank编号 | KF264451 |
| 启动子 | hybrid constitutive promoter 35SPPDK |
| 标签/融合蛋白: |
FLAG（插入N端子）
NLS（插入件上的N端子）
NLS（插接件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | PstI (未破坏) |
| 5' 测序引物 | sequencing from the promoter: 5’ gtcacgtagtaagcagctctcgg 3’ |
| 3' 测序引物 | sequencing from the terminator: 5’ atcgcaagaccggcaacagga 3’ |
|
文献引用方法 |
|
| 材料和方法部分: | HBT-pcoCas9 was a gift from Jen Sheen (Addgene plasmid # 52254 ; http://n2t.net/addgene:52254 ; RRID:Addgene_52254) |
| 参考文献部分: | Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM, Sheen J. Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654. 10.1038/nbt.2654 PubMed 23929339 |
买家导航
HBTpcoCas9载体基本信息
HBTpcoCas9载体图谱质粒图谱和多克隆位点信息
HBTpcoCas9载体简介
HBTpcoCas9载体序列
LOCUS HBT-pcoCas9 7940 bp ds-DNA circular SYN 20-SEP-2018
DEFINITION transient expression of pcoCas9 gene in plant cells.
ACCESSION .
VERSION .
KEYWORDS HBT-pcoCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7940)
AUTHORS Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM,
Sheen J
TITLE Multiplex and homologous recombination-mediated genome editing in
Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.
JOURNAL Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
PUBMED 23929339
REFERENCE 2 (bases 1 to 7940)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 20, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7940
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(39..55)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(39..55)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(52..74)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 63..79
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(87..117)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 132..153
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(270..287)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(441..1029)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(521..540)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1200..2060)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1823..1842
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2061..2165)
/gene="bla"
/label=AmpR promoter
primer_bind 2233..2251
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2289..2311)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2411..2430
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2624..2646
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2638..2655
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2639..2655
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2722..3231
/label=35SPPDK promoter
/note="hybrid promoter consisting of the cauliflower mosaic
virus 35S enhancer fused to the maize C4PPDK basal promoter
(Yoo et al., 2007)"
CDS 3243..3290
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/label=2xFLAG
/translation="DYKDDDDKDYKDDDDK"
CDS 3297..3317
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS join(3342..4778,4968..7631)
/codon_start=1
/product="Cas9 endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system"
/label=pcoCas9
/note="plant codon-optimized Cas9 gene containing the
potato IV2 intron"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
intron 4779..4967
/label=IV2 intron
/note="modified second intron of the potato ST-LS1 gene
(Vancanneyt et al., 1990)"
CDS 7632..7679
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
terminator 7688..7940
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
ORIGIN
1 cgatgataag ctgtcaaaca tgagaattcg taatcatggt catagctgtt tcctgtgtga
61 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
121 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
181 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
841 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
1021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
1081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
1141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
1201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
1261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
1321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
1381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
1441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
1501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
1561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
1621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
1681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
1741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
1801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
1861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
1921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
1981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
2041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
2101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
2161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
2221 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
2281 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
2341 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
2401 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
2461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
2521 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
2581 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
2641 aaaacgacgg ccagtgccaa gctctcgaga agcttactcc aagaatatca aagatacagt
2701 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
2761 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
2821 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
2881 cagtggtccc aaagatggac ccccacccac aaggagcatc gtggaaaaag aagacgttcc
2941 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
3001 acaatcccac tatccttcgc cccaagcttg ggcccaagct tgggtcgcgc cccacggatg
3061 gtataagaat aaaggcattc cgcgtgcagg attcacccgt tcgcctctca ccttttcgct
3121 gtactctctc gccacacaca ccccctctcc agctccgttg gagctccgga cagcagcagg
3181 cgcggggcgg tcacgtagta agcagctctc ggctccctct ccccttgctc cgtggatcca
3241 tggattacaa ggatgatgat gataaggatt acaaggatga tgatgataag atggctccaa
3301 agaagaagag aaaggttgga atccacggag ttccagctgc tgataagaag tactctatcg
3361 gacttgacat cggaaccaac tctgttggat gggctgttat caccgatgag tacaaggttc
3421 catctaagaa gttcaaggtt cttggaaaca ccgatagaca ctctatcaag aagaacctta
3481 tcggtgctct tcttttcgat tctggagaga ccgctgaggc taccagattg aagagaaccg
3541 ctagaagaag atacaccaga agaaagaaca gaatctgcta ccttcaggaa atcttctcta
3601 acgagatggc taaggttgat gattctttct tccacagact tgaggagtct ttccttgttg
3661 aggaggataa gaagcacgag agacacccaa tcttcggaaa catcgttgat gaggttgctt
3721 accacgagaa gtacccaacc atctaccacc ttagaaagaa gttggttgat tctaccgata
3781 aggctgatct tagacttatc taccttgctc ttgctcacat gatcaagttc agaggacact
3841 tccttatcga gggagacctt aacccagata actctgatgt tgataagttg ttcatccagc
3901 ttgttcagac ctacaaccag cttttcgagg agaacccaat caacgcttct ggagttgatg
3961 ctaaggctat cctttctgct agactttcta agtctcgtag acttgagaac cttatcgctc
4021 agcttccagg agagaagaag aacggacttt tcggaaacct tatcgctctt tctcttggac
4081 ttaccccaaa cttcaagtct aacttcgatc ttgctgagga tgctaagttg cagctttcta
4141 aggataccta cgatgatgat cttgataacc ttcttgctca gatcggagat cagtacgctg
4201 atcttttcct tgctgctaag aacctttctg atgctatcct tctttctgac atccttagag
4261 ttaacaccga gatcaccaag gctccacttt ctgcttctat gatcaagaga tacgatgagc
4321 accaccagga tcttaccctt ttgaaggctc ttgttagaca gcagcttcca gagaagtaca
4381 aggaaatctt cttcgatcag tctaagaacg gatacgctgg atacatcgat ggaggagctt
4441 ctcaggagga gttctacaag ttcatcaagc caatccttga gaagatggat ggaaccgagg
4501 agcttcttgt taagttgaac agagaggatc ttcttagaaa gcagagaacc ttcgataacg
4561 gatctatccc acaccagatc caccttggag agcttcacgc tatccttcgt agacaggagg
4621 atttctaccc attcttgaag gataacagag agaagatcga gaagatcctt accttcagaa
4681 tcccatacta cgttggacca cttgctagag gaaactctcg tttcgcttgg atgaccagaa
4741 agtctgagga gaccatcacc ccttggaact tcgaggaggt aagtttctgc ttctaccttt
4801 gatatatata taataattat cattaattag tagtaatata atatttcaaa tatttttttc
4861 aaaataaaag aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt
4921 taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggtt gttgataagg
4981 gagcttctgc tcagtctttc atcgagagaa tgaccaactt cgataagaac cttccaaacg
5041 agaaggttct tccaaagcac tctcttcttt acgagtactt caccgtttac aacgagctta
5101 ccaaggttaa gtacgttacc gagggaatga gaaagccagc tttcctttct ggagagcaga
5161 agaaggctat cgttgatctt cttttcaaga ccaacagaaa ggttaccgtt aagcagttga
5221 aggaggatta cttcaagaag atcgagtgct tcgattctgt tgaaatctct ggagttgagg
5281 atagattcaa cgcttctctt ggaacctacc acgatctttt gaagatcatc aaggataagg
5341 atttccttga taacgaggag aacgaggaca tccttgagga catcgttctt acccttaccc
5401 ttttcgagga tagagagatg atcgaggaga gactcaagac ctacgctcac cttttcgatg
5461 ataaggttat gaagcagttg aagagaagaa gatacaccgg atggggtaga ctttctcgta
5521 agttgatcaa cggaatcaga gataagcagt ctggaaagac catccttgat ttcttgaagt
5581 ctgatggatt cgctaacaga aacttcatgc agcttatcca cgatgattct cttaccttca
5641 aggaggacat ccagaaggct caggtttctg gacagggaga ttctcttcac gagcacatcg
5701 ctaaccttgc tggatctcca gctatcaaga agggaatcct tcagaccgtt aaggttgttg
5761 atgagcttgt taaggttatg ggtagacaca agccagagaa catcgttatc gagatggcta
5821 gagagaacca gaccacccag aagggacaga agaactctcg tgagagaatg aagagaatcg
5881 aggagggaat caaggagctt ggatctcaaa tcttgaagga gcacccagtt gagaacaccc
5941 agcttcagaa cgagaagttg tacctttact accttcagaa cggaagagat atgtacgttg
6001 atcaggagct tgacatcaac agactttctg attacgatgt tgatcacatc gttccacagt
6061 ctttcttgaa ggatgattct atcgataaca aggttcttac ccgttctgat aagaacagag
6121 gaaagtctga taacgttcca tctgaggagg ttgttaagaa gatgaagaac tactggagac
6181 agcttcttaa cgctaagttg atcacccaga gaaagttcga taaccttacc aaggctgaga
6241 gaggaggact ttctgagctt gataaggctg gattcatcaa gagacagctt gttgagacca
6301 gacagatcac caagcacgtt gctcagatcc ttgattctcg tatgaacacc aagtacgatg
6361 agaacgataa gttgatcaga gaggttaagg ttatcacctt gaagtctaag ttggtttctg
6421 atttcagaaa ggatttccag ttctacaagg ttagagagat caacaactac caccacgctc
6481 acgatgctta ccttaacgct gttgttggaa ccgctcttat caagaagtac ccaaagttgg
6541 agtctgagtt cgtttacgga gattacaagg tttacgatgt tagaaagatg atcgctaagt
6601 ctgagcagga gatcggaaag gctaccgcta agtacttctt ctactctaac atcatgaact
6661 tcttcaagac cgagatcacc cttgctaacg gagagatcag aaagagacca cttatcgaga
6721 ccaacggaga gaccggagag atcgtttggg ataagggaag agatttcgct accgttagaa
6781 aggttctttc tatgccacag gttaacatcg ttaagaaaac cgaggttcag accggaggat
6841 tctctaagga gtctatcctt ccaaagagaa actctgataa gttgatcgct agaaagaagg
6901 attgggaccc aaagaagtac ggaggattcg attctccaac cgttgcttac tctgttcttg
6961 ttgttgctaa ggttgagaag ggaaagtcta agaagttgaa gtctgttaag gagcttcttg
7021 gaatcaccat catggagcgt tcttctttcg agaagaaccc aatcgatttc cttgaggcta
7081 agggatacaa ggaggttaag aaggatctta tcatcaagtt gccaaagtac tctcttttcg
7141 agcttgagaa cggaagaaag agaatgcttg cttctgctgg agagcttcag aagggaaacg
7201 agcttgctct tccatctaag tacgttaact tcctttacct tgcttctcac tacgagaagt
7261 tgaagggatc tccagaggat aacgagcaga agcagctttt cgttgagcag cacaagcact
7321 accttgatga gatcatcgag caaatctctg agttctctaa gagagttatc cttgctgatg
7381 ctaaccttga taaggttctt tctgcttaca acaagcacag agataagcca atcagagagc
7441 aggctgagaa catcatccac cttttcaccc ttaccaacct tggtgctcca gctgctttca
7501 agtacttcga taccaccatc gatagaaaaa gatacacctc taccaaggag gttcttgatg
7561 ctacccttat ccaccagtct atcaccggac tttacgagac cagaatcgat ctttctcagc
7621 ttggaggaga taagagacca gctgctacca agaaggctgg acaggctaag aagaagaagt
7681 gactgcagat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
7741 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
7801 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
7861 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
7921 gtcatctatg ttactagatc
//
HBTpcoCas9载体图谱质粒图谱pdf版和相关资料下载
HBTpcoCas9载体质粒应用举例
- 上一篇：Flag-SIRT1 H363Y
- 下一篇：SQT1665
