pLZ361

LOCUS       pLZ361            8673 bp DNA     circular SYN 20-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     147..166
                     /label=olyl254
     primer_bind     213..257
                     /label=oLH198 EF1a F
     primer_bind     213..232
                     /label=oSCL771
     primer_bind     214..250
                     /label=olyl433
     misc_feature    232
                     /label=CMV
     misc_feature    234..1300
                     /label=P_EF1a
     primer_bind     234..258
                     /label=P_EF1a F
     primer_bind     234..250
                     /label=olyl254
     intron          353..1291
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1150..1170
                     /label=P-EF1a-SEQ-F
     primer_bind     complement(1271..1322)
                     /label=oLH199 EF1a R
     primer_bind     complement(1271..1300)
                     /label=P_EF1a R
     primer_bind     complement(1283..1326)
                     /label=olyl434
     primer_bind     complement(1291..1302)
                     /label=olyl253
     primer_bind     1301..1328
                     /label=oLH099 LoxP F
     promoter        1309..1313
                     /label=P_TAL
     primer_bind     1318..1363
                     /label=olyl285
     misc_recomb     1329..1362
                     /label=LoxP
     protein_bind    complement(1329..1362)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1366..1400
                     /label=olyl405
     primer_bind     complement(1370..1396)
                     /label=olyl406
     primer_bind     1403..1422
                     /label=olyl294
     polyA_signal    1418..1642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1629..1650)
                     /label=olyl286
     primer_bind     1631..1683
                     /label=olyl287
     terminator      1651..1835
                     /label=SV40 late polyA
     primer_bind     complement(1816..1840)
                     /label=olyl288
     primer_bind     1821..1862
                     /label=olyl289
     primer_bind     1872..1906
                     /label=olyl285
     misc_feature    1872..1905
                     /label=loxP
     protein_bind    complement(1872..1905)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1912..1960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(1932..1963)
                     /label=olyl290
     primer_bind     complement(1954..1996)
                     /label=oLH100 LoxP R
     primer_bind     1976..2000
                     /label=oSCL1563
     regulatory      1980..1989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1986..2048
                     /label=Igk secretion signal
     CDS             1986
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation=""
     CDS             1989
                     /codon_start=1
                     /label=mCherry
                     /translation=""
     primer_bind     2006..2025
                     /label=oYS520
     primer_bind     complement(2028..2054)
                     /label=oSCL1564
     primer_bind     2035..2073
                     /label=oSCL1739
     primer_bind     2048..2090
                     /label=oSCL1082
     CDS             2055..2642
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     2055..2073
                     /label=oSCL1162
     primer_bind     2072..2130
                     /label=oSCL1083
     primer_bind     2111..2169
                     /label=oSCL1084
     primer_bind     2148..2206
                     /label=oSCL1085
     primer_bind     2185..2243
                     /label=oSCL1086
     primer_bind     complement(2213..2232)
                     /label=oSCL1106
     primer_bind     2214..2252
                     /label=oSCL1104
     primer_bind     complement(2449..2507)
                     /label=oSCL1105
     primer_bind     complement(2489..2560)
                     /label=oSCL1087
     primer_bind     2491..2512
                     /label=oSCL1107
     primer_bind     2542..2560
                     /label=oSCL1088
     primer_bind     complement(2624..2662)
                     /label=oSCL1740
     primer_bind     complement(2624..2643)
                     /label=oSCL1089
     primer_bind     complement(2624..2643)
                     /label=oSCL1163
     primer_bind     complement(2643..2666)
                     /label=oSCL1564
     primer_bind     2643..2656
                     /label=oYS220
     primer_bind     2643..2656
                     /label=oYS326
     misc_feature    2649..3392
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    2922..2924
                     /label=F93A
                     /note="F93A"
     primer_bind     3348..3367
                     /label=oYS217
     primer_bind     complement(3348..3367)
                     /label=oYS218
     primer_bind     complement(3362..3392)
                     /label=oSCL1565
     primer_bind     complement(3362..3392)
                     /label=oSCL1570
     primer_bind     3376..3434
                     /label=oSCL1566
     misc_feature    3376..3392
                     /label=EpoR-D1_D2_TM
     misc_feature    3402..4230
                     /label=IL6ST
     misc_feature    3429..3455
                     /label=box1
     primer_bind     3736..3775
                     /label=oSCL1568
     primer_bind     complement(3736..3755)
                     /label=oSCL1567
     misc_feature    3753..3755
                     /label=Y759A
     primer_bind     complement(4215..4255)
                     /label=oSCL1569
     primer_bind     complement(4236..4255)
                     /label=oSCL1565
     primer_bind     complement(4237..4258)
                     /label=oLeo540_3.1_SfiI_R
     primer_bind     4258..4277
                     /label=olyl294
     polyA_signal    4273..4497
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4484..4505)
                     /label=olyl286
     primer_bind     4486..4506
                     /label=olyl287
     rep_origin      4543..4971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4985..5314
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    5165..5242
                     /label=SV40 ORI
     primer_bind     complement(5298..5319)
                     /label=oSCL1562
     CDS             5381..6175
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6349..6470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6440..6472)
                     /label=olyl287
     primer_bind     complement(6515..6535)
                     /label=M13-rev
     primer_bind     complement(6519..6535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6541..6563)
                     /label=LacO
     protein_bind    6543..6559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6567..6597)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(6568..6597)
                     /label=lac
     protein_bind    6612..6633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6739..7676
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7677..8537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8538..8642)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgggaaag
      241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      361 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      421 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggccggccga agcagaaggc
     1321 tagcgcttat aacttcgtat agcatacatt atacgaagtt atcggcgcgc caatgctccg
     1381 aatgcatcag tgcccctgca gggctgatca gcctcgactg tgccttctag ttgccagcca
     1441 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1501 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1561 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     1621 ggggatgcgg tgggctctat ggcttctgag gataagatac attgatgagt ttggacaaac
     1681 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1741 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1801 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc gcttggtacc gtctttccag
     1861 tggttaatta aataacttcg tatagcatac attatacgaa gttatcgatc cgaacaaacg
     1921 acccaacacc cgtgcgtttt attctgtctt tttattgccg atcctcgagg aattcgccgg
     1981 ccaccatgga gaccgatacc ttattactgt gggtgcttct tctgtgggtt cccggtagca
     2041 ccggagacgg atccaagaag aagggcagcg tggtgatcgt gggcagaatc aacctgagcg
     2101 gcgacaccgc ctacagccag cagaccagag gcctggaggg ctgccaggag accagccaga
     2161 ccggcagaga caagaaccag gtggagggcg aggtgcaggt ggtgagcacc gccacccaga
     2221 gcttcctggc caccagcatc aacggcgtgc tgtggaccgt gtaccacggc gccggcacca
     2281 gaaccatcgc cagccccaag ggccccgtga cccagatgta cacgaacgtg gacaaggact
     2341 tagtgggctg gcaggccccc caaggcagca gaagcctgac cccctgcacc tgcggcagca
     2401 gcgacctgta cctggtgacc agacacgccg acgtgatccc cgtgagaaga agaggcgaca
     2461 gcagaggcag cctgctgagc cccagaccca tcagctatct gaaaggctca agcggcggcc
     2521 ccctgttgtg tcccgccggc catgccgtgg gcatcttcag agccgccgtg agcaccagag
     2581 gcgtggccaa ggccgtggac ttcatccccg tggagagcct ggagaccacc atgagaagcc
     2641 ccgaattcgc accttcaccc agcctcccgg accccaagtt tgagagcaaa gcggccctgc
     2701 tggcatcccg gggctccgaa gaacttctgt gcttcaccca acgcttggaa gacttggtgt
     2761 gtttctggga ggaagcggcg agctccggga tggacttcaa ctacagcttc tcataccagc
     2821 tcgagggtga gtcacgaaag tcatgtagcc tgcaccaggc tcccaccgtc cgcggctccg
     2881 tgcgtttctg gtgttcactg ccaacagcgg acacatcgag tgctgtgccg ctggagctgc
     2941 aggtgacgga ggcgtccggt tctcctcgct atcaccgcat catccatatc aatgaagtag
     3001 tgctcctgga cgcccccgcg gggctgctgg cgcgccgggc agaagagggc agccacgtgg
     3061 tgctgcgctg gctgccacct cctggagcac ctatgaccac ccacatccga tatgaagtgg
     3121 acgtgtcggc aggcaaccgg gcaggaggga cacaaagggt ggaggtcctg gaaggccgca
     3181 ctgagtgtgt tctgagcaac ctgcggggcg ggacgcgcta caccttcgct gttcgagcgc
     3241 gcatggccga gccgagcttc agcggattct ggagtgcctg gtctgagccc gcgtcactac
     3301 tgaccgctag cgacctggac cctctcatct tgacgctgtc tctcattctg gtcctcatct
     3361 cgttgttgct gacggttctg gccctgctgt ccgcagcagc aaataagcga gacctaatta
     3421 aaaaacacat ctggcctaat gttccagatc cttcaaagag tcatattgcc cagtggtcac
     3481 ctcacactcc tccaaggcac aattttaatt caaaagatca aatgtattca gatggcaatt
     3541 tcactgatgt aagtgttgtg gaaatagaag caaatgacaa aaagcctttt ccagaagatc
     3601 tgaaatcatt ggacctgttc aaaaaggaaa aaattaatac tgaaggacac agcagtggta
     3661 ttggggggtc ttcatgcatg tcatcttcta ggccaagcat ttctagcagt gatgaaaatg
     3721 aatcttcaca aaacacttcg agcactgtcc aggcttctac cgtggtacac agtggctaca
     3781 gacaccaagt tccgtcagtc caagtcttct caagatccga gtctacccag cccttgttag
     3841 attcagagga gcggccagaa gatctacaat tagtagatca tgtagatggc ggtgatggta
     3901 ttttgcccag gcaacagtac ttcaaacaga actgcagtca gcatgaatcc agtccagata
     3961 tttcacattt tgaaaggtca aagcaagttt catcagtcaa tgaggaagat tttgttagac
     4021 ttaaacagca gatttcagat catatttcac aatcctgtgg atctgggcaa atgaaaatgt
     4081 ttcaggaagt ttctgcagca gatgcttttg gtccaggtac tgagggacaa gtagaaagat
     4141 ttgaaacagt tggcatggag gctgcgactg atgaaggcat gcctaaaagt tacttaccac
     4201 agactgtacg gcaaggcggc tacatgcctc agtaatctag agggcccgtt taaacccgct
     4261 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4321 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4381 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4441 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     4501 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4561 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4621 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4681 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4741 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4801 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4861 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4921 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4981 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5041 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5101 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5161 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5221 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5281 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5341 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     5401 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5461 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5521 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     5581 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5641 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5701 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5761 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5821 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5881 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5941 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     6001 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6061 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6121 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     6181 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6241 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6301 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6361 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6421 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6481 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6541 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6601 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6661 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6721 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6781 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6841 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6901 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6961 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7021 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7081 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7141 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7201 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7261 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7321 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7381 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7441 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8641 cgcacatttc cccgaaaagt gccacctgac gtc
//