pBE980a

LOCUS       pbe980a                 4562 bp DNA     circular SYN 23-JUL-2013
DEFINITION  bla gene was cutted from pBE980.
ACCESSION   pBE980a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Self
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished."
COMMENT     SECID/File created by SciEd Central, Scientific
COMMENT     VNTDATE|783561600|
COMMENT     VNTDBDATE|668304000|
COMMENT     VNTNAME|pBE980a|
FEATURES             Location/Qualifiers
     CDS             93..179
                     /codon_start=1
                     /gene="sig pep"
                     /label=sig pep
     CDS             573..1577
                     /codon_start=1
                     /gene="B. rep"
                     /product="replication protein"
                     /label=B. rep
     CDS             1737..2507
                     /codon_start=1
                     /gene="kanR"
                     /label=kanR
     CDS             2730..3128
                     /codon_start=1
                     /gene="bleoR"
                     /product="bleomycin resistance protein"
                     /label=bleoR
     misc_feature    complement(3525..4197)
                     /gene="E. ori"
                     /label=E. ori
     misc_feature    join(4506..4562,1..36)
                     /gene="p43 promoter"
                     /label=p43 promoter
ORIGIN
        1 cgcgattatg taaaatataa agtgatagcg gtaccaggag ggctggaaga agcagaccgc
       61 taacacagta cataaaaaag gagacatgaa cgatgaacat caaaaagttt gcaaaacaag
      121 caacagtatt aacctttact accgcactgc tggcaggagg cgcaactcaa gcttttgcct
      181 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc tagcttcagc
      241 acaattccaa gaaaaacacg atttagaacc taaaaagaac gaatttgaac taactcataa
      301 ccgagaggta aaaaaagaac gaagtcgaga tcagggaatg agtttataaa ataaaaaaag
      361 cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt tctttcttat cttgatacat
      421 atagaaataa cgtcattttt attttagttg ctgaaaggtg cgttgaagtg ttggtatgta
      481 tgtgttttaa agtattgaaa acccttaaaa ttggttgcac agaaaaaccc catctgttaa
      541 agttataagt gactaaacaa ataactaaat agatgggggt ttcttttaat attatgtgtc
      601 ctaatagtag catttattca gatgaaaaat caagggtttt agtggacaag acaaaaagtg
      661 gaaaagtgag accatggaga gaaaagaaaa tcgctaatgt tgattacttt gaacttctgc
      721 atattcttga atttaaaaag gctgaaagag taaaagattg tgctgaaata ttagagtata
      781 aacaaaatcg tgaaacaggc gaaagaaagt tgtatcgagt gtggttttgt aaatccaggc
      841 tttgtccaat gtgcaactgg aggagagcaa tgaaacatgg cattcagtca caaaaggttg
      901 ttgctgaagt tattaaacaa aagccaacag ttcgttggtt gtttctcaca ttaacagtta
      961 aaaatgttta tgatggcgaa gaattaaata agagtttgtc agatatggct caaggatttc
     1021 gccgaatgat gcaatataaa aaaattaata aaaatcttgt tggttttatg cgtgcaacgg
     1081 aagtgacaat aaataataaa gataattctt ataatcagca catgcatgta ttggtatgtg
     1141 tggaaccaac ttattttaag aatacagaaa actacgtgaa tcaaaaacaa tggattcaat
     1201 tttggaaaaa ggcaatgaaa ttagactatg atccaaatgt aaaagttcaa atgattcgac
     1261 cgaaaaataa atataaatcg gatatacaat cggcaattga cgaaactgca aaatatcctg
     1321 taaaggatac ggattttatg accgatgatg aagaaaagaa tttgaaacgt ttgtctgatt
     1381 tggaggaagg tttacaccgt aaaaggttaa tctcctatgg tggtttgtta aaagaaatac
     1441 ataaaaaatt aaaccttgat gacacagaag aaggcgattt gattcataca gatgatgacg
     1501 aaaaagccga tgaagatgga ttttctatta ttgcaatgtg gaattgggaa cggaaaaatt
     1561 attttattaa agagtagttc aacaaacggg ccagtttgtt gaagattaga tgctataatt
     1621 gttattaaaa ggattgaagg atgcttagga agacgagtta ttaatagctg aataagaacg
     1681 gtgctctcca aatattctta tttagaaaag caaatctaaa attatctgaa aagggaatga
     1741 gaatagtgaa tggaccaata ataatgacta gagaagaaag aatgaagatt gttcatgaaa
     1801 ttaaggaacg aatattggat aaatatgggg atgatgttaa ggctattggt gtttatggct
     1861 ctcttggtcg tcagactgat gggccctatt cggatattga gatgatgtgt gtcatgtcaa
     1921 cagaggaagc agagttcagc catgaatgga caaccggtga gtggaaggtg gaagtgaatt
     1981 ttgatagcga agagattcta ctagattatg catctcaggt ggaatcagat tggccgctta
     2041 cacatggtca atttttctct attttgccga tttatgattc aggtggatac ttagagaaag
     2101 tgtatcaaac tgctaaatcg gtagaagccc aaacgttcca cgatgcgatt tgtgccctta
     2161 tcgtagaaga gctgtttgaa tatgcaggca aatggcgtaa tattcgtgtg caaggaccga
     2221 caacatttct accatccttg actgtacagg tagcaatggc aggtgccatg ttgattggtc
     2281 tgcatcatcg catctgttat acgacgagcg cttcggtctt aactgaagca gttaagcaat
     2341 cagatcttcc ttcaggttat gaccatctgt gccagttcgt aatgtctggt caactttccg
     2401 actctgagaa acttctggaa tcgctagaga atttctggaa tgggattcag gagtggacag
     2461 aacgacacgg atatatagtg gatgtgtcaa aacgcatacc attttgaacg atgacctcta
     2521 ataattgtta atcatgttgg ttacgtattt attaacttct cctagtatta gtaattatca
     2581 tggctgtcat ggcgcattaa cggaataaag ggtgtgctta aatcgggcca ttttgcgtaa
     2641 taagaaaaag gattaattat gagcgaattg aattaataat aaggtaatag atttacatta
     2701 gaaaatgaaa ggggatttta tgcgtgagaa tgttacagtc tatcccggca ttgccagtcg
     2761 gggatattaa aaagagtata ggtttttatt gcgataaact aggtttcact ttggttcacc
     2821 atgaagatgg attcgcagtt ctaatgtgta atgaggttcg gattcatcta tgggaggcaa
     2881 gtgatgaagg ctggcgctct cgtagtaatg attcaccggt ttgtacaggt gcggagtcgt
     2941 ttattgctgg tactgctagt tgccgcattg aagtagaggg aattgatgaa ttatatcaac
     3001 atattaagcc tttgggcatt ttgcacccca atacatcatt aaaagatcag tggtgggatg
     3061 aacgagactt tgcagtaatt gatcccgaca acaatttgat tagctttttt caacaaataa
     3121 aaagctaaaa tctattatta atctgttcag caatcgggcg cgattgctga ataaaagata
     3181 cgagagacct ctcttgtatc ttttttattt tgagtggttt tgtccgttac actagaaaac
     3241 cgaaagacaa taaaaatttt attcttgctg agtctggctt tcggtaagct agacaaaacg
     3301 gacaaaataa aaattggcaa gggtttaaag gtggagattt tttgagtgat cttctcaaaa
     3361 aatactacct gtcccttgct gatttttaaa cgagcacgag agcaaaaccc ccctttgctg
     3421 aggtggcaga gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga aaggtcttaa
     3481 aggttttatg gttttggtcg gcactgaatt ctgaagctat ggtgtgtgag caaaaggcca
     3541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4381 ctgtctattt cgtcaaacat caccctcttg ctaaagcggc caaggacgct gccgccgggg
     4441 ctgtttgcgt ttttgccgtg atttcgtgta tcattggttt acttattttt ttgccaaagc
     4501 tgtaatggct gaaaattctt acatttattt tacattttta gaaatgggcg tgaaaaaaag
     4561 cg
//