pLVX-DD-tdTomato Control

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-DD-tdTomato Control
质粒类型: 慢病毒载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 9880 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: AcGFP1 (N-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-DD-tdTomato Control组成型表达DD-tdTomato,是pLVX-DD-tdTomato Reporter载体的阳性对照载体;
DD-tdTomato与tdTomato相比,N端融合了ProteoTuner destabilization domain (DD)去稳定结构域,导致tdTomato在细胞内很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。
产品目录号: 631754
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-DD-tdTomato Control载体图谱



pLVX-DD-tdTomato Control 载体特征

载体简介

载体序列

LOCUS       pLVX-DD-tdTomato Control   9833 bp    DNA   circular             6-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631753,631754
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    5801..6392
                     /label=WPRE
     rep_origin      complement(7701..8374)
                     /label=pUC\origin
     CDS             complement(8519..9515)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     misc_feature    2028..2151
                     /label=cPPT/CTS
     LTR             6595..7231
                     /label=3'\LTR
     misc_feature    4659..5167
                     /label=PGK\Promoter
     misc_feature    5188..5787
                     /label=Puro(R)
     CDS             2880..3203
                     /label=DD
     CDS             3210..4637
                     /label=tdTomato
BASE COUNT     2418 a      2609 c      2641 g      2165 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagaa 
     2881 tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc 
     2941 agacctgtgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtc gattcctccc 
     3001 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg 
     3061 aagaaggggt tgctcagatg agtgtgggtc agagagccaa actgactata tctccagatt 
     3121 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg 
     3181 atgtggagct tctaaaaccg gaaagatcta tggtgagcaa gggcgaggag gtcatcaaag 
     3241 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg 
     3301 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 
     3361 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca 
     3421 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg 
     3481 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg 
     3541 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc 
     3601 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc 
     3661 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg 
     3721 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc 
     3781 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca 
     3841 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg 
     3901 gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca 
     3961 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgaattcg 
     4021 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 
     4081 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg 
     4141 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc 
     4201 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga 
     4261 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca 
     4321 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg 
     4381 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga 
     4441 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc 
     4501 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact 
     4561 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca 
     4621 tggacgagct gtacaagtag gcggccgcga ctctagataa ttctaccggg taggggaggc 
     4681 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 
     4741 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 
     4801 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 
     4861 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 
     4921 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 
     4981 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 
     5041 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 
     5101 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 
     5161 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc 
     5221 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 
     5281 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 
     5341 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 
     5401 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 
     5461 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 
     5521 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 
     5581 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 
     5641 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 
     5701 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 
     5761 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa 
     5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 
     6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     6181 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 
     6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     6361 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa 
     6421 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta 
     6481 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca 
     6541 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa 
     6601 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 
     6661 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc 
     6721 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa 
     6781 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 
     6841 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg 
     6901 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg 
     6961 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc 
     7021 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg 
     7081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 
     7141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 
     7201 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 
     7261 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt 
     7321 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     7381 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     7441 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     7501 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     7561 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     7621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     7681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     7741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     7801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     7861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     7921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     7981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     8041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     8101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     8161 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
     8221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     8281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     8341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     8401 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     8461 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     8521 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     8581 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     8641 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     8701 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     8761 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     8821 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     8881 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     8941 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     9001 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     9061 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     9121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     9181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     9241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     9301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     9361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     9421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     9481 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 
     9541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     9601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     9661 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 
     9721 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 
     9781 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt 
//

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