pKOR1

价格:6000元

联系方式:I47-825O-882O

买家导航

pKORI载体基本信息

出品公司: --
载体名称: pKOR1
质粒类型: 金黄色葡萄球菌基因敲除载体,大肠杆菌-金黄色葡萄球菌穿梭载体
高拷贝/低拷贝: 高拷贝
克隆方法: Gateway克隆
启动子: --
载体大小: 10030 bp
5' 测序引物及序列: 自己合成
3' 测序引物及序列: 自己合成
载体标签: --
载体抗性: 氨苄青霉素/氯霉素
筛选标记: 氯霉素(10 ug/ml)
克隆菌株: DH5α 等
宿主细胞(系): 金黄色葡萄球菌
备注: pKOR1是大肠杆菌-金黄色葡萄球菌穿梭载体,主要用于金葡菌的基因敲除。pKOR1金黄色葡萄球菌基因敲除载体,在大肠杆菌中的抗性为Amp,在金葡菌中筛选抗性为氯霉素(10ug/ml).

生物风对金黄色葡萄球菌的基因敲除具有丰富的实验经验,并且成功敲除过很多金黄色葡萄球菌基因,请有需要对金黄色葡萄球菌进行基因操作技术服务的老师和我们联系。
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pKORI载体质粒图谱和多克隆位点信息

pKOR1载体图谱
pKOR1载体图谱


pKOR1金黄色葡萄球菌基因敲除实验流程图:

pKOR1载体图谱

pKORI载体简介

pKOR1是大肠杆菌-金黄色葡萄球菌穿梭载体,主要用于金葡菌的基因敲除。

pKOR1金黄色葡萄球菌基因敲除载体,在大肠杆菌中的抗性为Amp,在金葡菌中筛选抗性为氯霉素(10ug/ml).

生物风对金黄色葡萄球菌的基因敲除具有丰富的实验经验,并且成功敲除过很多金黄色葡萄球菌基因,请有需要对金黄色葡萄球菌进行基因操作技术服务的老师和我们联系。

pKORI载体序列

LOCUS       pKOR1                  10030 bp    DNA     circular      2-OCT-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTDATE|705424862|
COMMENT     VNTDBDATE|746737717|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pKOR1|
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     primer          9998..10015
                     /vntifkey="27"
                     /label=MM13\Forword
     misc_feature    4824..4840
                     /vntifkey="21"
                     /label=M13-\R\primer
     misc_feature    complement(7345..7361)
                     /vntifkey="21"
                     /label=M13-R\Primer
     rep_origin      complement(7783..8411)
                     /vntifkey="33"
                     /label=ColE1\origin
     CDS             complement(9859..9927)
                     /vntifkey="4"
                     /label=LacZ\alpha\Reporter
     misc_binding    complement(7367..7389)
                     /vntifkey="20"
                     /label=LacO
     CDS             8563..9222
                     /vntifkey="4"
                     /label=Amp\R
     CDS             1033..1368
                     /vntifkey="4"
                     /label=Rep(+)
     CDS             complement(1966..2592)
                     /vntifkey="4"
                     /label=Tet\Resistance
     misc_feature    complement(2722..3294)
                     /vntifkey="21"
                     /label=SecY-F
     misc_feature    5362..6045
                     /vntifkey="21"
                     /label=Cm\Resistance
     misc_feature    4883..5114
                     /vntifkey="21"
                     /label=attP2
     CDS             6365..6670
                     /vntifkey="4"
                     /label=ccdB
     misc_feature    complement(7066..7297)
                     /vntifkey="21"
                     /label=attP2
     misc_feature    4883..5114
                     /vntifkey="21"
                     /label=attP1
     misc_feature    complement(7066..7297)
                     /vntifkey="21"
                     /label=attP1
BASE COUNT     3069 a      1935 c      2129 g      2897 t 
ORIGIN
        1 ctgcagaacg gattgttgat gattacgaaa atattaagag cacagactat tacacagaaa 
       61 atcaagaatt aaaaaaacgt agagagagtt tgaaagaagt agtgaataca tggaaagagg 
      121 ggtatcacga aaaaagtaaa gaggttaata aattaaagcg agagaatgat agtttgaatg 
      181 agcagttgaa tgtatcagag aaatttcaag ctagtacagt gactttatat cgtgctgcga 
      241 gggcgaattt ccctgggttt gagaaagggt ttaataggct taaagagaaa ttctttaatg 
      301 attccaaatt tgagcgtgtg ggacagttta tggatgttgt acaggataat gtccagaagg 
      361 tcgatagaaa gcgtgagaaa cagcgtacag acgatttaga gatgtagagg tacttttatg 
      421 ccgagaaaac tttttgcgtg tgacagtcct taaaatatac ttagagcgta agcgaaagta 
      481 gtagcgacag ctattaactt tcggttgcaa agctctagga tttttaatgg acgcagcgca 
      541 tcacacgcaa aaaggaaatt ggaataaatg cgaaatttga gatgttaatt aaagaccttt 
      601 ttgaggtctt tttttcttag atttttgggg ttatttaggg gagaaaacat aggggggtac 
      661 tacgacctcc cccctaggtg tccattgtcc attgtccaaa caaataaata aatattgggt 
      721 ttttaatgtt aaaaggttgt tttttatgtt aaagtgaaaa aaacagatgt tgggaggtac 
      781 agtgatagtt gtagatagaa aagaagagaa aaaagttgct gttactttaa gacttacaac 
      841 agaagaaaat gagatattaa atagaatcaa agaaaaatat aatattagca aatcagatgc 
      901 aaccggtatt ctaataaaaa aatatgcaaa ggaggaatac ggtgcatttt aaacaaaaaa 
      961 agatagacag cactggcatg ctgcctatct atgactaaat tttgttaaat gtattagcac 
     1021 cgttattata tcatgagcga aaatgtaata aaagaaactg aaaacaagaa aaattcaaga 
     1081 ggacgtaatt ggacatttgt tttatatcca gaatcagcaa aagccgagtg gttagagtat 
     1141 ttaaaagagt tacacattca atttgtagtg tctccattac atgataggga tactgataca 
     1201 gaagatagga tgaaaaaaga gcattatcat attctagtga tgtatgaggg taataaatct 
     1261 tatgaacaga taaaaataat taacagaaga attgaatgcg actattccgc agattgcagg 
     1321 aagtgtgaaa ggtcttgtga gatatatgct tcacatggac gatcctaata aatttaaata 
     1381 tcaaaaagaa gatatgatag tttatggcgg tgtagatgtt gatgaattat taaagaaaac 
     1441 aacaacagat agatataaat taattaaaga aatgattgag tttattgatg aacaaggaat 
     1501 cgtagaattt aagagtttaa tggattatgc aatgaagttt aaatttgatg attggttccc 
     1561 gcttttatgt gataactcgg cgtatgttat tcaagaatat ataaaatcaa atcggtataa 
     1621 atctgaccga tagattttga atttaagagt gtcacaagac actctttttt cgcaccagcg 
     1681 aaaactggtt taagccgact gcgcaaaaga cataatcgat tcacaaaaaa taggcacacg 
     1741 aaaaacaagt taagggatgc agtttatgca tcccttaact tacttattaa ataatttata 
     1801 gctattgaaa agagataaga attgttcaaa gctaatattg tttaaatcgt caattcctgc 
     1861 atgttttaag gaattgttaa attgattttt tgtaaatatt ttcttgtatt ctttgttatc 
     1921 ttggttaccg tgaagttacc atcacggaaa aaggttatgc tgcttttaag acccactttc 
     1981 acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg 
     2041 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc 
     2101 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca 
     2161 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc 
     2221 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg 
     2281 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt 
     2341 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg 
     2401 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg 
     2461 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc 
     2521 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa 
     2581 tctagacatc attaattcct cctttttgtt gacactctat cattgataga gttatttgtc 
     2641 aaactagttt tttatttgga tcccctcgag ttcatgaaaa actaaaaaaa atattgacac 
     2701 tctatcattg atagagtata attaaaataa gcttgatggg aatatgataa tagaaatacc 
     2761 attaccaaca ccgaactgag tgatttgatc accaagccat attaagaaag cagttcctgc 
     2821 tgtcaaaact agtgctatta ataaataact cataattgac tgattgataa tcagcgcacc 
     2881 tttgagataa ttattaaatt ggaatgccat acctatagat tggataaatg ctaaagaaat 
     2941 tgctaaataa cgagtaacgt tatttaactt tcttctacct acttcacctt gttttgccca 
     3001 ttctgagaat ttagggacaa tatccatttg taataattgc attacgattg atgcagtgat 
     3061 gtagggtaca atacccattg caaaaataga aaatcgtttc aaggctccgc caccaaaagt 
     3121 atttaataac tcagtggcac cttgagaacc ttggggatta tcaaaagctg caggatttac 
     3181 tcctggagct ggtatataag tccctatttt aaaaattact aacattgcta gtgtgaagaa 
     3241 aatcttgtta cgaacctctt ttgttctaaa gaagttcaca agggtttgaa tcattagatc 
     3301 accccggggt gatctaatga ttcatatcta catcaaatgc tggggcatat gttttaccct 
     3361 caggtacagg taagtcaccc gcttcaaacg tcttcttaat atgcgtaatt gcatctactc 
     3421 taaatccatc aatgccttta tcaaaccacc agttcatcat ttcaaataca gcatctctaa 
     3481 cttccggatt accccaattc aaatcaggtt gttttttact gaataaatgg aaataatatt 
     3541 gctcagtatt agcatcatat tcccgcgcgc caattgagct cccctttcta tgtatgtttt 
     3601 ttactagtca tttaaaacga tacattaata ggtacgaaaa agcaactttt tttgcgctta 
     3661 aaaccagtca taccaataac ttaagggtaa ctagcctcgc cggcaatagt tacccttatt 
     3721 atcaagataa gaaagaaaag gatttttcgc tacgctcaaa tcctttaaaa aaacacaaaa 
     3781 gaccacattt tttaatgtgg tcttttattc ttcaactaaa gcacccatta gttcaacaaa 
     3841 cgaaaattgg ataaagtggg atatttttaa aatatatatt tatgttacag taatattgac 
     3901 ttttaaaaaa ggattgattc taatgaagaa agcagacaag taagcctcct aaattcactt 
     3961 tagataaaaa tttaggaggc atatcaaatg aaatttaata aaattgattt agacaattgg 
     4021 aagagaaaag agatatttaa tcattatttg aaccaacaaa cgacttttag tataaccaca 
     4081 gaaattgata ttagtgtttt ataccgaaac ataaaacaag aaggatataa attttaccct 
     4141 gcatttattt tcttagtgac aagggtgata aactcaaata cagcttttag aactggttac 
     4201 aatagcgacg gagagttagg ttattgggat aagttagagc cactttatac aatttttgat 
     4261 ggtgtatcta aaacattctc tggtatttgg actcctgtaa agaatgactt caaagagttt 
     4321 tatgatttat acctttctga tgtagagaaa tataatggtt cggggaaatt gtttcccaaa 
     4381 acacctatac ctgaaaatgc tttttctctt tctattattc catggacttc atttactggg 
     4441 tttaacttaa atatcaataa taatagtaat taccttctac ccattattac agcaggaaaa 
     4501 ttcattaata aaggtaattc aatatattta ccgctatctt tacaggtaca tcattctgtt 
     4561 tgtgatggtt atcatgcagg attgtttatg aactctattc aggaattgtc agataggcct 
     4621 aatgactggc ttttataata tgagataatg ccgactgtac tttttacagt cggttttcta 
     4681 atgtcactaa cctgccccgt tagttgaaga aggtttttat attacagctc cagatccata 
     4741 tccttctttt tctgaaccga cttctccttt ttcgcttctt tattccaatt gctttattga 
     4801 cgttgagcct cggaaccggt acccaggaaa cagctatgac catgtaatac gactcactat 
     4861 acggggatat cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg 
     4921 ttgcaacaaa ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctgaa 
     4981 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag 
     5041 actacataat actgtaaaac acaacatatc cagtcactat gaatcaacta cttagatggt 
     5101 attagtgacc tgtagtcgac taagttggca gcatcacccg acgcactttg cgccgaataa 
     5161 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc 
     5221 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc 
     5281 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt 
     5341 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 
     5401 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 
     5461 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 
     5521 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc 
     5581 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 
     5641 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 
     5701 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 
     5761 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 
     5821 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 
     5881 ggcaaatatt atacgcaagg cgacaaggtg ctgatgacgc tggcgattca ggttcatcat 
     5941 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 
     6001 gagtggcagg gggggcgtaa acgccgcgtg gatccggctt actaaaagcc agataacagt 
     6061 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa 
     6121 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag 
     6181 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg 
     6241 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg 
     6301 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg 
     6361 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga 
     6421 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagcgc 
     6481 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga 
     6541 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga 
     6601 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg 
     6661 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgat acagtagaaa 
     6721 ttacagaaac tttatcacgt ttagtaagta tagaggctga aaatccagat gaagccgcac 
     6781 gacttgtaag agaaaagtat aagagttgtg aaattgttct tgatgcagat gattttcagg 
     6841 actatgacac tagcgtatat gaataggtag atgtttttat tttgtcacac aaaaaagagg 
     6901 ctcgcacctc tttttcttat ttctttttat gatttaatac ggcattgagg acaatagcga 
     6961 gtaggctgga tacgacgatt ccgtttgaga agaacatttg gaaggctgtc ggtcgactaa 
     7021 gttggcagca tcacccgaag aacatttgga aggctgtcgg tcgactacag gtcactaata 
     7081 ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta ttatgtagtc 
     7141 tgttttttat gcaaaatcta atttaatata ttgatattta tatcacttta cgtttctcgt 
     7201 tcagcttttt tgtacaaagt tggcattata aaaaagcatt gctcatcaat ttgttgcaac 
     7261 gaacaggtca ctatcagtca aaataaaatc attatttggg gcccgagctt aagactggcc 
     7321 gtcgttttac gaattcgtga ctatgtcata gctgtttcct gtgtgaaatt gttatccgct 
     7381 cacaattcca cacaacatac gagccggaag cataaagtga aagcctgggg tgcctaaaat 
     7441 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     7501 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     7561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     7621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     7681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     7741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     7801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     7861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     7921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     7981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     8041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     8101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     8161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     8221 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     8281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     8341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     8401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     8461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     8521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     8581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     8641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     8701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     8761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     8821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     8881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     8941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     9001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     9061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     9121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     9181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     9241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     9301 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     9361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     9421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     9481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     9541 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 
     9601 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 
     9661 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 
     9721 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 
     9781 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 
     9841 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 
     9901 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 
     9961 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 
    10021 gcttgcatgc 
//

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