| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-DD-AcGFP1-Actin |
| 质粒类型: | 慢病毒载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 10219 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | DD-AcGFP (N-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-DD-AcGFP1-Actin组成型表达N端DD-AcGFP1标记的肌动蛋白Actin； DD标签是一个大小为12KD的FKBP (L106P)去稳定结构域，带有DD标签的目的蛋白将很快被降解； 向培养基中加入Shield1配体后，细胞中目的蛋白的量则迅速提高。 |
| 产品目录号: | 631077 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pLVX-DD-AcGFP1-Actin is an HIV-1-based, lentiviral vector that expresses the ligand-dependent, destabilized fusion protein DD-AcGFP1-Actin. This protein is composed of human cytoplasmic b-actin fused to AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens (excitation and emission maxima: 475 nm and 505 nm, respectively; 1). The fusion protein also contains an N-terminal ProteoTuner destabilization domain (DD; 2), which—in the absence of the stabilizing ligand Shield1— causes rapid, proteasomal degradation of the fusion protein. When added to the culture medium, Shield1 binds to the destabilization domain, preventing degradation of DD-AcGFP1-Actin. The stabilized fusion protein can then be incorporated into growing actin filaments, allowing actin-containing subcellular structures to be visualized in living and fixed cells (3, 4).
pLVX-DD-AcGFP-Actin contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (5), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (6). Finally, pLVX-DD-AcGFP-Actin also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (7).
In addition to lentiviral elements, pLVX-DD-AcGFP-Actin contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.
pLVX-DD-AcGFP1-Actin, available as part of the Lenti-X Actin Dynamics Monitoring Kit (Cat. No. 631077), allows you to monitor how newly synthesized, Shield1-stabilized DD-AcGFP1-Actin is integrated into the dynamic actin filament network. pLVX-DD-AcGFP-Actin expresses a DD-AcGFP-Actin fusion protein when transduced into target cells; the fusion protein is degraded until Shield1 is added to the culture medium. The stabilized fusion protein is incorporated into actin filaments, allowing the visualization of actin-containing subcellular structures 15–20 minutes after the addition of Shield1 to the medium.
Before the vector can be transduced, it must be transfected into 293T packaging cells with our Lenti-X HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (8). If required, stable transfectants can be selected using puromycin.
Propagation in E. coli
Recommended host strains: DH5α and other general purpose strains.
Selectable marker: plasmid confers resistance to ampicillin (100 μg/ml) in E. coli hosts.
E. coli replication origin: pUC
Copy number: high
Excitation and emission maxima of AcGFP1
Excitation maximum = 475 nm
Emission maximum = 505 nm
References
1. Ponte, P. et al. (1984) Nucleic Acids Res. 12(3):1687–1696.
2. Banaszynski, L. et al. (2006) Cell 126(5):995–1004.
3. Westphal, M. et al. (1997) Curr. Biol. 7(3):176–183.
4. de Hostos, E. L., unpublished data.
5. Zufferey, R. et al. (1999) J. Virol. 73(4):2886–2892.
6. Cochrane, A. W. et al. (1990) Proc. Natl. Acad. Sci. USA 87(3):1198–1202.
7. Zennou, V. et al. (2000) Cell 101(2):173–185.
5. Wu, X. et al. (2000) Mol. Ther. 2(1):47–55.
载体序列
LOCUS pLVX-DD-AcGFP1-Actin 10222 bp DNA circular 21-DEC-2009
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 631076,631077
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
promoter 2185..2787
/label=CMV\IE\Promoter
misc_feature 6190..6781
/label=WPRE
rep_origin complement(8090..8763)
/label=pUC\origin
CDS complement(8908..9904)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
misc_feature 2031..2046
/label=cPPT
LTR 6984..7620
/label=LTR
misc_feature 5048..5556
/label=PGK\promoter
misc_feature 5577..6176
/label=Puro(R)
CDS 2821..3144
/label=DD
CDS 3175..3891
/label=AcGFP1
CDS 3913..5037
/label=Actin
BASE COUNT 2504 a 2724 c 2709 g 2285 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
2821 atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc
2881 cagacctgtg tggtgcacta caccgggatg cttgaagatg gaaagaaagt cgattcctcc
2941 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
3001 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
3061 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
3121 gatgtggagc ttctaaaacc ggaagaattc tgcagtcgac ggtaccgcgg gcccatggtg
3181 agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct gaatggcgat
3241 gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac ctacggcaag
3301 ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc caccctggtg
3361 accaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat gaagcagcac
3421 gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat cttcttcgag
3481 gatgacggca actacaagtc gcgcgccgag gtgaagttcg agggcgatac cctggtgaat
3541 cgcatcgagc tgaccggcac cgatttcaag gaggatggca acatcctggg caataagatg
3601 gagtacaact acaacgccca caatgtgtac atcatgaccg acaaggccaa gaatggcatc
3661 aaggtgaact tcaagatccg ccacaacatc gaggatggca gcgtgcagct ggccgaccac
3721 taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa ccactacctg
3781 tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat gatctacttc
3841 ggcttcgtga ccgccgccgc catcacccac ggcatggatg agctgtacaa gtccggactc
3901 agatctcgag ctatggatga tgatatcgcc gcgctcgtcg tcgacaacgg ctccggcatg
3961 tgcaaggccg gcttcgcggg cgacgatgcc ccccgggccg tcttcccctc catcgtgggg
4021 cgccccaggc accagggcgt gatggtgggc atgggtcaga aggattccta tgtgggcgac
4081 gaggcccaga gcaagagagg catcctcacc ctgaagtacc ccatcgagca cggcatcgtc
4141 accaactggg acgacatgga gaaaatctgg caccacacct tctacaatga gctgcgtgtg
4201 gctcccgagg agcaccccgt gctgctgacc gaggcccccc tgaaccccaa ggccaaccgc
4261 gagaagatga cccagatcat gtttgagacc ttcaacaccc cagccatgta cgttgctatc
4321 caggctgtgc tatccctgta cgcctctggc cgtaccactg gcatcgtgat ggactccggt
4381 gacggggtca cccacactgt gcccatctac gaggggtatg ccctccccca tgccatcctg
4441 cgtctggacc tggctggccg ggacctgact gactacctca tgaagatcct caccgagcgc
4501 ggctacagct tcaccaccac ggccgagcgg gaaatcgtgc gtgacattaa ggagaagctg
4561 tgctacgtcg ccctggactt cgagcaagag atggccacgg ctgcttccag ctcctccctg
4621 gagaagagct acgagctgcc tgacggccag gtcatcacca ttggcaatga gcggttccgc
4681 tgccctgagg cactcttcca gccttccttc ctgggcatgg agtcctgtgg catccacgaa
4741 actaccttca actccatcat gaagtgtgac gtggacatcc gcaaagacct gtacgccaac
4801 acagtgctgt ctggcggcac caccatgtac cctggcattg ccgacaggat gcagaaggag
4861 atcactgccc tggcacccag cacaatgaag atcaagatca ttgctcctcc tgagcgcaag
4921 tactccgtgt ggatcggcgg ctccatcctg gcctcgctgt ccaccttcca gcagatgtgg
4981 atcagcaagc aggagtatga cgagtccggc ccctccatcg tccaccgcaa atgcttctag
5041 tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
5101 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
5161 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
5221 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
5281 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
5341 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
5401 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
5461 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
5521 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
5581 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
5641 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
5701 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
5761 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
5821 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
5881 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
5941 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
6001 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
6061 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
6121 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaccgc
6181 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
6241 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
6301 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
6361 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
6421 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
6481 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
6541 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
6601 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
6661 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
6721 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
6781 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
6841 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
6901 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
6961 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
7021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
7081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
7141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
7201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
7261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
7321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
7381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
7441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
7501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
7561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
7621 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
7681 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
7741 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
7801 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
7861 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
7921 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
7981 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
8041 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
8101 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
8161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
8221 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
8281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
8341 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
8401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
8461 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
8521 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
8581 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
8641 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
8701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
8761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
8821 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
8881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
8941 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
9001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
9061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
9121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
9181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
9241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
9301 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
9361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
9421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
9481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
9541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
9601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
9661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
9721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
9781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
9841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
9901 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
9961 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
10021 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
10081 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
10141 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
10201 tatgtactac aaacttagta gt
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