pAAV-fNPY-GFP

价格:1000元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pAAV-fNPY-GFP 
出品公司: 爱迪基因
目录编号: 22912
存储实验室: Edward Callaway 

载体骨架基本信息
载体名称: pAAV
载体抗性: 氨苄霉素
空载体大小: 4000 bp
完整质粒大小: 8300 bp
载体类型: AAV,哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: fugu neuropeptide Y promoter driving GFP
物种来源: takifugu rubripes (河豚)
插入大小: 4300 bp
克隆方法: 限制性内切酶
5' 测序引物 Unknown
3' 测序引物 SP6

文献引用方法
材料和方法部分: pAAV-fNPY-GFP was a gift from Edward Callaway (爱迪基因 plasmid # 22912)
参考文献部分: Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461

载体图谱质粒图谱和多克隆位点信息

pAAV-fNPY-GFP质粒图谱

载体简介

载体序列

LOCUS       pAAV-fNPY-GFP                8248 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-fNPY-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009  . 3():19.
  PUBMED    19949461
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pAAV-fNPY-GFP|
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          2874..3349
                     /label=-beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     3431..3447
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3431..3447
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      3480..3489
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3486..4202
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3531..3552)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3792..3811)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4139..4160
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4263..4281)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4264..4281)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4301..4318)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4307..4418
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4562..4691
                     /label=AAV2 ITR
     rep_origin      5062..5575
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5149..5168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5359..5380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6057..6076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6176..6198
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6236..6254)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6322..6426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6427..7287
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6645..6664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7458..8046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7947..7966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8200..8217
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctctccccgc gcgttggccg attcattaat gcagctgcgc gctcgctcgc tcactgaggc
       61 cgcccgggca aagcccgggc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      121 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacg
      181 tagccatgct ctagaggatc cttcgaaaag cttgtatacg gccggccacg cgtatgaagg
      241 gagggaaatt cagaaccaag cgaatacaat aagaaagaaa tacaaaagaa aagcaaattg
      301 acttggtgct tgatttctga tgtttcaacg tattttcaca catgatagca tatggttgct
      361 atggagaagg gatgcctcat tttgtccata attacagttt attttagttt ttttttttct
      421 aattgactta agcaaccgga gctacattgg aggtttttgc tatcttatca ctgatattat
      481 tagtgctgat gttaatcatt attaatggac atattaccct gactcccact tagcatcgcc
      541 atttctctct cctcgagctg gaaaagatac aaaatcctcc tgtatgctca tagaaatcct
      601 gccaaattct tccaaaatct gatctgcttg tcagagctat tttaattata gaaggtatgg
      661 tgaatattta attaatcaag ctgattgaga tggtctaatc cgctgtttgc tcgtgtgaag
      721 acagcagcaa atgaaggaaa gatgttgttt tattgccgat gttgatgcgt atccttcgac
      781 actcgcgccg tttcccggct gacgtgcaga gggttcaggc tcggatcccc gtttcctgtg
      841 tggactctcc ctcttctccc cgttcctgca catcccgctt cactgttctg ttcctgccct
      901 gcccgtcgga gtgttgacgg tcatttgttc ctccatctgg gcccactgat ggattcgcct
      961 ccacctccca gataccagat attcgctggt ttcactgcat cttcgtgtta ttaatgcgat
     1021 gctgtttccc tccctggagc agatgtttga gatggcaaag aatattttcc catttcacac
     1081 cccacccccc ctgtaaatgg agggctgaga ctaagaagcc attgtagcag ctcttcttgg
     1141 tcatctccta ttttgagtgt gaaggtgatg gcgcctcacc ttttcctacc aaaggacagg
     1201 atatcaccag gaagaaacag ctattttaga taaaaatgat gaatatttaa caccccgttt
     1261 ggattttcat gttgtttttg cctttgtgcg atgacatcag tgcactttta agattttttt
     1321 tcttcttcct tgtagtttaa aggccttttc aatcgtggta ggggtgttac atttaactta
     1381 gaagtaatta cacacactcc ttgcctcctt tgacggctgc tgtgactcca aactcatgct
     1441 cctgggcttg aacccgtctg ctccagctgc ttggatctca acagatcacc tgtttatcag
     1501 cctggagctg tgaagagcgc actgcaaaca caggctgacc tctcacccct gcaatcaggc
     1561 acattttaat ttcaagccct tcactccaac gagcccgtaa gacgtcgtca ttctgtgcat
     1621 gtgtggactc ggttctggtt ccacgcacgt tctcactcgt catctctctt tgagcatcct
     1681 gagttgtaca aaaaaagccc ctaacgcatc ctccaggagc agcacggctg gataatggtg
     1741 atttttgacg ctccaagcat gcgtacaaaa ggccgatgaa tgggaagaag atgaagcact
     1801 tagtgatgca gactcatctg aagccttatt tgggttttct tattaagggg gttgattgac
     1861 aggcctccat attcatgttt agggcttttg ttcacattga agacttcgag tccctctttt
     1921 ccatctgatg cagaagggat gggggggggg ggggggataa gcgatctaat tcatcacaca
     1981 tcccctcaag ttagtgtgca cgcgagtctg gctgactctt ttcatttctc gcgcttcttt
     2041 cagggaggaa gactcaagtt tggttgcagc tcttttgtga cttcagatgt gattattgat
     2101 ctcattatgc agaagaaatg cgtcttgatt gtgaaacctc agagacaccg aagctcccac
     2161 actcagcacg agtgtttatc tgcgttgtgt gtgtgtgtgt gcgtgtgtgt gtgtgtgtgt
     2221 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtac acgcacctgc gtctgagttg
     2281 ccctatcaaa attggtttaa tggtgtttcc aggtgcaaat cacagtgcac cgtttagctg
     2341 ttgattaatt cccccctttt ttaacaccct aaattctctc tttagctgct ggaatgggca
     2401 gatttgctcg tctccctcca tcctcggcct ctcgggttct tctttacgct gttctacgtg
     2461 ttttatatta aaagaccccg tttaaaaggt gacagcatca ccagtgacgt ccacacggag
     2521 aatgcagagg aactctgacg cgctgttcca cagccccttt gcgcgcacat ataaatccca
     2581 ccgcgcagcc agccgccacc aaagtcgagg agacgccacg ggattacgca caaagccacc
     2641 ctgaaccact gggaaacgta agtactttgc tttcaaatct gaaaaattaa aacttctcaa
     2701 aaggtgcgac tttgttaatc tgtttgaagg gcgattaaag caactcaatt taacaaaatc
     2761 gcagattatc cccgggtttt aaatatattg gggaaagaaa tgtatataaa atagatgtga
     2821 ttttagatgg gtacgctagc ggcgcgccga gctcggatcc tgagaacttc agggtgagtc
     2881 tatgggaccc ttgatgtttt ctttcccctt cttttctatg gttaagttca tgtcatagga
     2941 aggggagaag taacagggta cacatattga ccaaatcagg gtaattttgc atttgtaatt
     3001 ttaaaaaatg ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc
     3061 ctaatctctt tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct
     3121 aaagaataac agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat
     3181 ttctgcatat aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc
     3241 cagctaccat tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag
     3301 ctaggccctt ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa
     3361 cgtgctggtc tgtgtgctgg cccatcactt tggcaaagca cgtgaattcg aagatctggc
     3421 cgcctcggcc tctagaacta gtggatcccc cgggctgcag gaattcgatg ctaccggtcg
     3481 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3541 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3601 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3661 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3721 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3781 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3841 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3901 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3961 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4021 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4081 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4141 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4201 agtccggact cagatcctac tagtaggatc tcgagtttaa accgtacggg aaacggccct
     4261 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     4321 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4381 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4441 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4501 gcggccgctc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac
     4561 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4621 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4681 cgagcgcgca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4741 cgcagcctga atggcgaatg gcgattccgt tgcaatggct ggcggtaata ttgttctgga
     4801 tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa
     4861 tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg
     4921 cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc
     4981 tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt
     5041 gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5101 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5161 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5221 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5281 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5341 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5401 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5461 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttaaa
     5521 tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata
     5581 tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct
     5641 caggcaatga cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat
     5701 gaatttatca gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct
     5761 ttctcacccg tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga
     5821 gggttctaaa aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca
     5881 gggtcataat gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa
     5941 ttttgctaat tctttgcctt gcctgtatga tttattggat gttggaatcg cctgatgcgg
     6001 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     6061 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     6121 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6181 agctgcatgt gtcagaggtt ttcaccatca tcaccgaaac gcgcgagacg aaagggcctc
     6241 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     6301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6661 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8101 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8161 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8221 gcggaagagc gcccaatacg caaaccgc
//

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