pK18mobSacB

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pK18mobSacB
质粒类型: 芽孢杆菌基因敲除载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5.6kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Kanamycin
筛选标记: --
备注: --
产品目录号: --
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

载体简介

Cloning vector allowing mobilization into a wide range of Gramand
Gram+ bacteria. After
mobilization, the plasmid can be maintained by integration into the host chromosome via
homologous recombination. Excision of the intervening plasmid sequence by a double crossover
event can be facilitated by selection on medium containing 10% sucrose.The sacB gene has
been modified to eliminate the HindIII and EcoRI sites in the coding region.
Designation: pK18mobsacB

载体序列

LOCUS       pK18mobsacB   5721 bp   DNA                       17-AUG-2006
DEFINITION  Cloning vector pK18mobsacB.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Cloning vector pK18mobsacB.
  ORGANISM  Cloning vector pK18mobsacB
            Unclassified.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Schafer,A., Tauch,A., Jager,W., Kalinowski,K., Thierbach,G. and
            Puhler,A.
  TITLE     Small mobilizable multi-purpose cloning vectors derived from the
            Escherichia coli plasmids pK18 and pK19: selection of defined
            deletions in the chromosome of Corynebacterium glutamicum
  JOURNAL   Gene 145, 69-73 (1994)
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Ishikawa,J.
  TITLE     Unofficial nucleotide sequence of pK18mobsacB
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5721)
  AUTHORS   Ishikawa,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2006) Bioactive Molecules, NIID
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Cloning vector pK18mobsacB"
                     /mol_type="Other DNA"
     CDS             complement(185..469)
                     /gene="lacZ"
                     /codon_start=1
                     /product="beta-galactosidase"
     oriT            complement(2209..2736)
                     /note="RP4 origin of transfer"
     CDS             complement(2886..4307)
                     /gene="sacB"
                     /codon_start=1
                     /product="levansucrase"
     CDS             complement(4799..5593)
                     /gene="neo"
                     /note="confers resistances to neomycin and kanamycin"
                     /codon_start=1
                     /product="aminoglycoside 3'-O-phosphotransferase"
BASE COUNT     1343 a   1426 c   1414 g   1538 t
ORIGIN
        1 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc
       61 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
      121 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct
      181 tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgaagctagc ttatcgcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1561 cttttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccc
     1621 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag
     1681 ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc
     1741 gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg
     1801 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
     1861 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctgatag aaacagaagc
     1921 cactggagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccg
     1981 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc
     2041 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
     2101 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
     2161 tttttgagtt atcgagattt tcaggagctc tttggcatcg tctctcgcct gtcccctcag
     2221 ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga tattccacaa aacagcaggg
     2281 aagcagcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     2341 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     2401 tggtgtatcc aacggcgtca gcggggcagg ataggtgaag taggcccacc cgcgagcggg
     2461 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     2521 cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc
     2581 aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga
     2641 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc
     2701 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gggcgtcccg
     2761 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     2821 tggcaggttg ggcgtcgctt ggtcggtcat ttcgctcggt accatcggca ttttcttttg
     2881 cgtttttatt tgttaactgt taattgtcct tgttcaagga tgctgtcttt gacaacagat
     2941 gttttcttgc ctttgatgtt cagcaggaag ctcggcgcaa acgttgattg tttgtctgcg
     3001 tagaatcctc tgtttgtcat atagcttgta atcacgacat tgtttccttt cgcttgaggt
     3061 acagcgaagt gtgagtaagt aaaggttaca tcgttaggat caagatccat ttttaacaca
     3121 aggccagttt tgttcagcgg cttgtatggg ccagttaaag aattagaaac ataaccaagc
     3181 atgtaaatat cgttagacgt aatgccgtca atcgtcattt ttgatccgcg ggagtcagtg
     3241 aacaggtacc atttgccgtt cattttaaag acgttcgcgc gttcaatttc atctgttact
     3301 gtgttagatg caatcagcgg tttcatcact tttttcagtg tgtaatcatc gtttagctca
     3361 atcataccga gagcgccgtt tgctaactca gccgtgcgtt ttttatcgct ttgcagaagt
     3421 ttttgacttt cttgacggaa gaatgatgtg cttttgccat agtatgcttt gttaaataaa
     3481 gattcttcgc cttggtagcc atcttcagtt ccagtgtttg cttcaaatac taagtatttg
     3541 tggcctttat cttctacgta gtgaggatct ctcagcgtat ggttgtcgcc tgagctgtag
     3601 ttgccttcat cgatgaactg ctgtacattt tgatacgttt ttccgtcacc gtcaaagatt
     3661 gatttataat cctctacacc gttgatgttc aaagagctgt ctgatgctga tacgttaact
     3721 tgtgcagttg tcagtgtttg tttgccgtaa tgtttaccgg agaaatcagt gtagaataaa
     3781 cggatttttc cgtcagatgt aaatgtggct gaacctgacc attcttgtgt ttggtctttt
     3841 aggatagaat catttgcatc gaatttgtcg ctgtctttaa agacgcggcc agcgtttttc
     3901 cagctgtcaa tagaagtttc gccgactttt tgatagaaca tgtaaatcga tgtgtcatcc
     3961 gcatttttag gatctccggc taatgcaaag acgatgtggt agccgtgata gtttgcgaca
     4021 gtgccgtcag cgttttgtaa tggccagctg tcccaaacgt ccaggccttt tgcagaagag
     4081 atatttttaa ttgtggacga atcaaattca gaaacttgat atttttcatt tttttgctgt
     4141 tcagggattt gcagcatatc atggcgtgta atatgggaaa tgccgtatgt ttccttatat
     4201 ggcttttggt tcgtttcttt cgcaaacgct tgagttgcgc ctcctgccag cagtgcggta
     4261 gtaaaggtta atactgttgc ttgttttgca aactttttga tgttcatcgt tcatgtctcc
     4321 ttttttatgt actgtgttag cggtctgctt cttccagccc tcctgtttga agatggcaag
     4381 ttagttacgc acaataaaaa aagacctaaa atatgtaagg ggtgacgcca aagtatacac
     4441 tttgcccttt acacatttta ggtcttgcct gctttatcag taacaaaccc gcgcgattta
     4501 cttttcgacc tcattctatt agactctcgt ttggattgca actggtctat tttcctcttt
     4561 tgtttgatag aaaatcataa aaggatttgc agactacggg cctaaagaac taaaaaatct
     4621 atctgtttct tttcattctc tgtatttttt atagtttctg ttgcatgggc ataaagttgc
     4681 ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata atagtgaacg
     4741 gcaggtatat gtgatgggtt aaaaaggatc gatcctctag cgaaccccag agtcccgctc
     4801 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     4861 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     4921 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     4981 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     5041 cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga
     5101 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     5161 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     5221 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     5281 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     5341 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     5401 ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     5461 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     5521 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     5581 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     5641 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     5701 taccagaggg cgccccagct g
//

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