pGEX-4T-3

价格:800元

联系方式:I47-825O-882O

买家导航

pGEX4T3载体基本信息

出品公司: GE
别名: pGEX-4T-3, pGEX4T3, pGEX 4T 3
质粒类型: 大肠杆菌蛋白表达载体
表达水平: 高拷贝
启动子: Tac
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4968 bp
5' 测序引物及序列: pGEX5':  GGGCTGGCAAGCCACGTTTGGTG
3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG
载体标签: N-GST
载体抗性: 氨苄
备注:
复制子是pMB1
产品目录号: 27-4583-01
稳定性: 瞬时表达
组成型: 诱导表达
病毒/非病毒: 非病毒

pGEX4T3载体质粒图谱和多克隆位点信息

pGEX-4T-3载体图谱和多克隆位点

pGEX4T3载体简介

pGEX4T3载体序列

LOCUS       pGEX-4T-3	4968 bp 	DNA     circular	SYN
DEFINITION  pGEX-4T-3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="pGEX-4T-3"
                     /mol_type="other DNA"
     promoter        184..212
                     /label="tac_promoter"
     misc_feature    224..246
                     /label="M13_pUC_rev_primer"
     gene            258..976
                     /label="GST (variant)"
                     /gene="GST (variant)"
     CDS             258..980
                     /label="ORF frame 3"
     misc_feature    complement(1018..1040)
                     /label="pGEX_3_primer"
     promoter        1306..1334
                     /label="AmpR_promoter"
     gene            1376..2236
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1376..2236
                     /label="ORF frame 2"
     rep_origin      2391..3010
                     /label="pBR322_origin"
     misc_feature    3308..4399
                     /label="lacI"
     CDS             3440..4399
                     /label="ORF frame 2"
     promoter        4448..4477
                     /label="lac_promoter"
     misc_feature    4491..4513
                     /label="M13_pUC_rev_primer"
     promoter        4512..4530
                     /label="M13_reverse_primer"
     CDS             4522..81
                     /label="ORF frame 1"
     misc_feature    4539..4694
                     /label="lacZ_a"
     promoter        complement(4542..4558)
                     /label="M13_forward20_primer"
     misc_feature    complement(4551..4573)
                     /label="M13_pUC_fwd_primer"
ORIGIN
    1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG
   61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT
  121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC
  181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA
  241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC
  301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC
  361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC
  421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA
  481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC
  541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT
  601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG
  661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT
  721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA
  781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT
  841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
  901 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGAA TTCCCGGGTC GACTCGAGCG
  961 GCCGCATCGT GACTGACTGA CGATCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT
 1021 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG
 1081 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA
 1141 GTCACGTAGC GATAGCGGAG TGTATAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT
 1201 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG
 1261 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
 1321 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG
 1381 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT
 1441 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT
 1501 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA
 1561 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT
 1621 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA
 1681 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG
 1741 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG
 1801 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG
 1861 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC
 1921 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG
 1981 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC
 2041 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG
 2101 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC
 2161 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT
 2221 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA
 2281 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA
 2341 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
 2401 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
 2461 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
 2521 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA
 2581 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
 2641 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
 2701 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
 2761 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
 2821 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
 2881 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
 2941 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
 3001 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT
 3061 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC
 3121 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG
 3181 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TAAATTCCGA
 3241 CACCATCGAA TGGTGCAAAA CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA
 3301 ATTCAGGGTG GTGAATGTGA AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT
 3361 CTCTTATCAG ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG
 3421 GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA
 3481 ACTGGCGGGC AAACAGTCGT TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC
 3541 GCCGTCGCAA ATTGTCGCGG CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT
 3601 GGTGTCGATG GTAGAACGAA GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT
 3661 CGCGCAACGC GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC
 3721 TGTGGAAGCT GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC
 3781 CATCAACAGT ATTATTTTCT CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT
 3841 CGCATTGGGT CACCAGCAAA TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG
 3901 TCTGCGTCTG GCTGGCTGGC ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA
 3961 ACGGGAAGGC GACTGGAGTG CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA
 4021 GGGCATCGTT CCCACTGCGA TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG
 4081 CGCCATTACC GAGTCCGGGC TGCGCGTTGG TGCGGATATC TCGGTAGTGG GATACGACGA
 4141 TACCGAAGAC AGCTCATGTT ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT
 4201 GCTGGGGCAA ACCAGCGTGG ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG
 4261 CAATCAGCTG TTGCCCGTCT CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA
 4321 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
 4381 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
 4441 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
 4501 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGGATT CACTGGCCGT CGTTTTACAA
 4561 CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT
 4621 TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC
 4681 AGCCTGAATG GCGAATGGCG CTTTGCCTGG TTTCCGGCAC CAGAAGCGGT GCCGGAAAGC
 4741 TGGCTGGAGT GCGATCTTCC TGAGGCCGAT ACTGTCGTCG TCCCCTCAAA CTGGCAGATG
 4801 CACGGTTACG ATGCGCCCAT CTACACCAAC GTAACCTATC CCATTACGGT CAATCCGCCG
 4861 TTTGTTCCCA CGGAGAATCC GACGGGTTGT TACTCGCTCA CATTTAATGT TGATGAAAGC
 4921 TGGCTACAGG AAGGCCAGAC GCGAATTATT TTTGATGGCG TTGGAATT
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