TetR-ELK1

LOCUS       TetR-ELK1              4605 bp DNA     circular SYN 13-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             864..1481
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     CDS             1482..1877
                     /codon_start=1
                     /label=ELK1
                     /translation="GPAVRSQPQKGRKPRDLELPLSPSLLGGPGPERTPGSGSGSGLQA
                     PGPALTPSLLPTHTLTPVLLTPSSLPPSIHFWSTLSPIAPRSPAKLSFQFPSSGSAQVH
                     IPSISVDGLSTPVVLSQGPRSHEPVPGN"
     polyA_signal    2017..2151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2689..3629
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(3630..4490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4491..4595)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
       61 aggcgtatca cgaggccctt tcgtcttcac tcgaggagct tggcccattg catacgttgt
      121 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      841 gcctccgcgg ccccgaattc accatgtcta gactggacaa gagcaaagtc ataaactctg
      901 ctctggaatt actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
      961 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     1021 atgccctggc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     1081 agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac
     1141 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     1201 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     1261 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggatcag gagcatcaag
     1321 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     1381 caattgagct gttcgaccat cagggagccg aacctgcctt ccttttcggc ctggaactaa
     1441 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gtacgctccc
     1501 agccgcagaa gggccggaag ccccgggacc tagagcttcc actcagcccg agcctgctag
     1561 gtgggccggg acccgaacgg accccaggat cgggaagtgg ctccggcctc caggctccgg
     1621 ggccggcgct gaccccatcc ctgcttccta cgcatacatt gaccccggtg ctgctgacac
     1681 ccagctcgct gcctcctagc attcacttct ggagcaccct gagtcccatt gcgccccgta
     1741 gcccggccaa gctctccttc cagtttccat ccagtggcag cgcccaggtg cacatccctt
     1801 ctatcagcgt ggatggcctc tcgacccccg tggtgctctc ccagggcccc agaagccatg
     1861 aaccggtccc gggtaactaa gtaaggatcc agacatgata agatacattg atgagtttgg
     1921 acaaaccaca actagaatgc agtgaaaaaa atgctttatt gtggtgtttt cccagggccc
     1981 cagaagccat gacccggtcc cgggtaacta agtaaggatc cagacatgat aagattcatt
     2041 gatgagtttg gacaacccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2101 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2161 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     2221 taaaacctct acaaatgtgg tatggctgat tatgatcctg caagcctcgt cgtcctggcc
     2281 ggaccacgct atctgtgcaa ggtccccggc cccggacgcg cgctccatga gcagagcgcc
     2341 cgccgccgag gcgaagactc gggcggcgcc ctgcccgtcc caccaggtca acaggcggta
     2401 accggcctct tcatcgggaa tgcgcgcgac cttcagcatc gccggcatgt ccccctggcg
     2461 gacgggaagt atccagctcg accaagcttg gcgagatttt caggagctaa ggaagctaaa
     2521 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     2581 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctgcat
     2641 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2701 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2761 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2821 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2881 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2941 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3001 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3061 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3121 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3181 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3241 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3301 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3361 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3421 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3481 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3541 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3601 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3661 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3721 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3781 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3841 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3901 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3961 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4021 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4081 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4141 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4201 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     4261 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4321 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4381 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4441 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4501 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4561 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccc
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