| 质粒名称: | pCMV-mChilada-iPAK4 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000477 |
| 产品用途: | Fluorescent Tickertape Expression |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
|
插入基因信息 |
|
| 插入基因名称: | mChilada-iPAK4 |
| 物种来源: |
synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pCMV-mChilada-iPAK4
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-mChilada-iPAK4载体质粒基本信息
pCMV-mChilada-iPAK4质粒图谱载体图谱和pCMV-mChilada-iPAK4载体序列质粒序列多克隆位点信息
pCMV-mChilada-iPAK4质粒载体简介
pCMV-mChilada-iPAK4质粒序列载体序列
LOCUS pCMV-mChilada-iPAK4 6658 bp DNA circular SYN 23-APR-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6658)
AUTHORS Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
TITLE Cerulean, Venus, and VenusY67C FRET reference standards.
JOURNAL Biophys J. 2006 Dec 15. 91(12):L99-L101.
PUBMED 17040988
REFERENCE 2 (bases 1 to 6658)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J.
2006 Dec 15. 91(12):L99-L101."
FEATURES Location/Qualifiers
source 1..6658
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 1387..2463
/mol_type="genomic DNA"
/organism="."
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 591..671
/label=MCS
/note="multiple cloning site"
primer_bind 646..684
/label=BamHI-iPAK4-F
primer_bind 652..697
/label=mScarletI-1P0K-F
primer_bind complement(669..695)
/label=mScarletI-FLAG-Qch-R
regulatory 673..682
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 679..1386
/codon_start=1
/label=mChilada
/translation="MVSKGEEDNMAIIKEYMRFKVHMEGSVNGHEFEIEGEGEGRPFEG
TQTAKLKVTKGGPLPFAWHILPPQFQYGSKAYVKHPADIPDYFKLSFPEGFTWEREMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGNTASTERMYPEDGALK
GETKWRLKLKDGGHYEAEVKTTYKAKKPVQLPGAYNVDRKLKITYHNEDYTIVEQYERA
EARHSTGGMDELYK"
primer_bind complement(1366..1412)
/label=XFP-iPAK4-R
CDS 1399..1512
/codon_start=1
/label=INKA-box
/translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
CDS 1519..2460
/codon_start=1
/label=Pak4cat
/translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
primer_bind complement(1612..1630)
/label=PAK4-R
primer_bind complement(2442..2482)
/label=NotI-PAK4-R
primer_bind 2552..2570
/label=Primer 2
polyA_signal 2585..2706
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2622..2641)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2670..2706)
/label=Primer 2
primer_bind 2676..2695
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2713..3168)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2850..2871)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3062..3081
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3195..3299
/gene="bla"
/label=AmpR promoter
primer_bind 3283..3327
/label=Primer 1
CDS 3300..4160
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4134..4178)
/label=Primer 3
promoter 4162..4519
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(4185..4205)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4370..4505
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4432..4451
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(4455..4466)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4554..5348
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4608..4627)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5218..5237
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5536..5555)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5580..5627
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5956..6544
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6445..6464
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg acaacatggc tatcatcaag
721 gaatacatgc gtttcaaggt tcatatggaa ggttctgtta acggtcatga attcgaaatc
781 gaaggtgaag gtgaaggtcg tcctttcgaa ggtacccaga ccgctaagct gaaggttacc
841 aagggtggtc ctctgccttt cgcttggcat atcctgcctc ctcagttcca gtacggttct
901 aaggcttacg ttaagcatcc tgctgacatc cctgactact tcaagctgtc tttccctgaa
961 ggtttcacct gggaacgtga aatgaacttc gaagacggtg gtgttgttac cgttacccag
1021 gactcttctc tgcaggacgg tgaattcatc tacaaggtta agctgcgtgg taccaacttc
1081 ccttctgacg gtcctgttat gcagaagaag accatgggta acaccgcttc taccgaacgt
1141 atgtaccctg aagacggtgc tctgaagggt gaaaccaagt ggcgtctgaa gctgaaggac
1201 ggtggtcatt acgaagctga agttaagacc acctacaagg ctaagaagcc tgttcagctg
1261 cctggtgctt acaacgttga ccgtaagctg aagatcacct accataacga agactacacc
1321 atcgttgaac agtacgaacg tgctgaagct cgtcattcta ccggtggcat ggacgagctg
1381 tacaagtcag gtggatccga agcagaggac tggacagcag ccctactgaa caggggtcgc
1441 agtcgccagc ccctggtact aggggacaat tgctttgctg acttggtgca caactggatg
1501 gagctgcctg aggaattccc cgccgcccct gctgttcctg ggccccctgg cccccgctca
1561 ccacagcggg agccacagcg agtatcccat gagcagttcc gggctgccct gcagctggtg
1621 gtggacccag gcgacccccg ctcctacctg gacaacttca tcaagattgg cgagggctcc
1681 acgggcatcg tgtgcatcgc caccgtgcgc agctcgggca agctggtggc cgtcaagaag
1741 atggacctgc gcaagcagca gaggcgcgag ctgctcttca acgaggtggt aatcatgagg
1801 gactaccagc acgagaatgt ggtggagatg tacaacagct acctggtggg ggacgagctc
1861 tgggtggtca tggagttcct ggaaggaggc gccctcaccg acatcgtcac ccacaccagg
1921 atgaacgagg agcagatcgc agccgtgtgc cttgcagtgc tgcaggccct gtcggtgctc
1981 cacgcccagg gcgtcatcca ccgggacatc aagagcgact cgatcctgct gacccatgat
2041 ggcagggtga agctgtcaga ctttgggttc tgcgcccagg tgagcaagga agtgccccga
2101 aggaagtcgc tggtcggcac gccctactgg atggccccag agctcatctc ccgccttccc
2161 tacgggccag aggtagacat ctggtcgctg gggataatgg tgattgagat ggtggacgga
2221 gagcccccct acttcaacga gccacccctc aaagccatga agatgattcg ggacaacctg
2281 ccaccccgac tgaagaacct gcacaaggtg tcgccatccc tgaagggctt cctggaccgc
2341 ctgctggtgc gagaccctgc ccagcgggcc acggcagccg agctgctgaa gcacccattc
2401 ctggccaagg cagggccgcc tgccagcatc gtgcccctca tgcgccagaa ccgcaccaga
2461 tgaagcggcc gcgactctag acataatcag ccataccaca tttgtagagg ttttacttgc
2521 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
2581 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
2641 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
2701 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
2761 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
2821 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
2881 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
2941 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
3001 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
3061 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
3121 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
3181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
3241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
3301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
3361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
3421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
3481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
3541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
3601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
3661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
3721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
3781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
3841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
3901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
3961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggctctc
4021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
4081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
4141 cactgattaa gcattggtaa cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
4201 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
4261 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
4321 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
4381 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
4441 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
4501 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
4561 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
4621 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
4681 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
4741 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
4801 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
4861 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
4921 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
4981 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
5041 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
5101 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
5161 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
5221 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
5281 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
5341 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
5401 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
5461 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
5521 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
5581 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
5641 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
5701 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
5761 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
5821 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
5881 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
5941 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
6001 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
6061 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
6121 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
6181 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
6241 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
6301 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
6361 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
6421 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
6481 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
6541 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
6601 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
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