pLVX-rHom-1

价格:4220元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pLVX-rHom-1
质粒类型: 慢病毒载体;双顺反子载体;信号通路载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8848 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢毒载体pLVX-rHom-1载体表达DmrD融合蛋白;利用信号受体的可逆二聚化作用研究细胞信号通路。
产品目录号: 635077
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-rHom-1载体图谱
pLVX-rHom-1 多克隆位点

pLVX-rHom-1 载体特征

载体简介

载体序列

LOCUS       pLVX-rHom-1   8848 bp    DNA   circular            10-NOV-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Catalog Nos. 635077, 635076
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=CTS/cppt\(DNA\flap)
     promoter        2185..2788
                     /label=pCMV
     misc_feature    4770..5361
                     /label=WPRE
     rep_origin      complement(6671..7341)
                     /label=pUC\origin
     CDS             complement(7486..8482)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             5565..6201
                     /label=3'\LTR
     misc_feature    3549..4124
                     /label=IRES
     CDS             4157..4756
                     /label=Puro(R)
     CDS             2849..3172
                     /label=DmrD
     CDS             3176..3499
                     /label=DmrD
BASE COUNT     2267 a      2210 c      2294 g      2077 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 
     2821 tatgccagaa gccgccacca tggcttctag aggagtgcag gtggagacta tctccccagg 
     2881 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct 
     2941 tgaagatgga aagaaaatgg attcctcccg ggacagaaac aagcccttta agtttatgct 
     3001 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca 
     3061 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat 
     3121 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaactagagg 
     3181 agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac 
     3241 ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaaatggatt cctcccggga 
     3301 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga 
     3361 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc 
     3421 ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt 
     3481 ggagcttcta aaactggaaa ctagttatcc gtacgacgta ccaagcggcc gcggatccta 
     3541 cgtatgaccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat 
     3601 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg 
     3661 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc 
     3721 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt 
     3781 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg 
     3841 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac 
     3901 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg 
     3961 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg 
     4021 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc 
     4081 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac 
     4141 aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 
     4201 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 
     4261 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 
     4321 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 
     4381 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 
     4441 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 
     4501 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 
     4561 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 
     4621 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 
     4681 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 
     4741 caagcccggt gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa 
     4801 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     4861 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     4921 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     4981 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     5041 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     5101 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     5161 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     5221 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     5281 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     5341 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc 
     5401 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 
     5461 ggaggaggag gtgggttttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 
     5521 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 
     5581 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 
     5641 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 
     5701 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 
     5761 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 
     5821 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 
     5881 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 
     5941 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 
     6001 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 
     6061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 
     6121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 
     6181 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 
     6241 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac 
     6301 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     6361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     6421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     6481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     6541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     6601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     6661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     6721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     6781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     6841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     6901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     6961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     7021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     7081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     7141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     7201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     7261 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     7321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     7381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     7441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     7501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     7561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     7621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     7681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     7741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     7801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     7861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     7921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     7981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     8041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     8101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     8161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     8221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     8281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     8341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     8401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     8461 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata 
     8521 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     8581 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 
     8641 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 
     8701 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 
     8761 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 
     8821 gttaaatatg tactacaaac ttagtagt 
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng