| 质粒名称: | pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85434 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR SIN |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Pembrolizumab |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry was a gift from Wendell Lim (Addgene plasmid # 85434 ; http://n2t.net/addgene:85434 ; RRID:Addgene_85434) |
| 参考文献部分: | Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim WA. Cell. 2016 Oct 6;167(2):419-432.e16. doi: 10.1016/j.cell.2016.09.011. Epub 2016 Sep 29. 10.1016/j.cell.2016.09.011 PubMed 27693353 |
pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_ligh
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_
Pembrolizumab_lightc... 12269 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and
constitutive mCherry expression.
ACCESSION .
VERSION .
KEYWORDS pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_
Pembrolizumab_lightc...
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12269)
AUTHORS Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH,
Walker WJ, McNally KA, Lim WA
TITLE Engineering T Cells with Customized Therapeutic Response Programs
Using Synthetic Notch Receptors.
JOURNAL Cell. 2016 Oct 6;167(2):419-432.e16. doi:
10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
PUBMED 27693353
REFERENCE 2 (bases 1 to 12269)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12269
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 58..87
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1453..1506
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 1564..1590
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter 2370..2869
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2405..2428)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2802..2821
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 2890..3600
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(3035..3053)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3303..3322
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 3677..4265
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3730..3750)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4148..4159)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4328..4561
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(4586..4604)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4587..4604)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4894..4913)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(5127..5146)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5246..5268
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5306..5324)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5392..5496
/gene="bla"
/label=AmpR promoter
CDS 5497..6357
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5715..5734)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6528..7116
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7017..7036
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7270..7287
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(7357..7377)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7362..7691
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7542..7677
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7604..7623
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 8825..8890
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9020..9040
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9465..9599
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9502..9521)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9556..9575
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 9767..10400
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 10447..10572
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 11069..11302
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 11816..11933
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 12159..12183
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 12264..4
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
ORIGIN
1 atggattgga catggagagt gttttgtctg cttgcggtga cacctggcgc tcatcctgag
61 cagaagctga tcagcgagga ggacctgcag gtacaactgg tccagtcagg cgtcgaggtt
121 aaaaagcccg gtgcctcagt gaaggtctcc tgcaaagcca gtggttacac cttcactaat
181 tactatatgt attgggtcag acaggcaccc ggccagggtc ttgaatggat gggaggcatc
241 aatcccagca atggagggac aaacttcaat gaaaagttca aaaatcgcgt gaccctcaca
301 accgactcct ccacaacaac agcatatatg gaactgaagt ctcttcagtt tgatgacacc
361 gccgtctact actgcgcccg acgagactac cggttcgata tgggattcga ctattggggt
421 caggggacga cagtgacagt ctcctccgca tctacaaagg gaccatcagt gtttcctctg
481 gccccgtgct cccgctctac cagcgaatct accgcagcat tgggctgttt ggtgaaggac
541 tacttccctg agccagtgac cgtgtcctgg aacagcgggg cactcactag cggagtgcac
601 acatttccag ccgtgcttca aagcagcggt ctctattccc tctccagcgt ggtgactgtg
661 ccatccagct ctctcggcac gaagacctat acctgcaacg tggaccacaa gccttcaaat
721 accaaggtgg ataaacgcgt cgaatccaaa tatggcccac cctgcccgcc atgtccagct
781 ccagagtttc tcggcgggcc tagcgtgttc ttgtttcccc ccaaacccaa ggacacgctc
841 atgatttcca gaacacctga agttacctgc gtcgtcgtag acgtcagtca ggaggacccg
901 gaagttcagt ttaactggta cgtggacggt gttgaggtgc ataatgccaa aaccaagcca
961 agagaagagc agttcaattc tacatatcga gtggtgtcag tcctgacagt tctccaccag
1021 gactggttga acggcaaaga gtacaaatgc aaggtctcca acaaaggatt gccgagcagt
1081 atagagaaaa caattagtaa agctaagggt cagcccagag agccccaggt gtacacgttg
1141 ccaccttcac aggaagagat gacgaagaac caagtgtctc tgacctgcct ggtgaagggc
1201 ttctacccat cagacattgc agtggagtgg gaaagcaatg ggcagcctga gaacaactac
1261 aaaactacac cccccgtcct ggacagcgat ggcagtttct tcctgtactc aaggttgact
1321 gtggacaaaa gcagatggca ggaaggcaat gtgttcagct gtagtgtgat gcacgaggcc
1381 ctccacaatc attacactca gaaatccctc tctctgagcc tgggaaagag gaagcgacgg
1441 agtgggtcag gcgaggggag aggctcactc ctcacgtgtg gagacgttga agaaaacccc
1501 ggaccaatgg cttggagtcc tctgttcctg accctgatta ctcactgcgc tggaagctgg
1561 gcttacccat acgatgttcc agattacgct gagattgttc tgacccaatc tcctgcaaca
1621 ctgagcctgt cccccggaga gagggcaacc ctctcctgcc gcgccagtaa gggagtgtct
1681 acgtctggat attcctatct ccattggtat cagcagaagc ctggccaggc gcccaggctt
1741 ctcatctacc ttgcttccta tctggagtcc ggggtgccag cccgcttttc tgggtccggc
1801 tctggtaccg acttcaccct gacaatttcc tcactggagc ctgaggattt cgctgtctac
1861 tattgtcaac attccagaga ccttcccctg acctttggag ggggtacaaa agtggagata
1921 aaacgaacag tggccgcccc atctgtattc atctttcctc catctgatga acagcttaag
1981 tccggcactg cttctgttgt ttgcctgctt aacaattttt acccgcggga agccaaggtg
2041 caatggaaag tggacaacgc cctgcagtct ggtaacagtc aggaaagcgt gactgaacag
2101 gacagcaaag actccacata ctccttgagt tccaccttga cgctcagtaa ggcagactac
2161 gagaagcaca aggtttatgc ctgtgaagtg actcaccagg gcctctctag cccagtgact
2221 aagtccttca accgcgggga gtgttgagat ccttgacttg cggccgcaac tcccacctgc
2281 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcatctag gcgccggaat
2341 tagatctctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
2401 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
2461 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
2521 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
2581 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
2641 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
2701 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
2761 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
2821 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
2881 aagcttacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
2941 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
3001 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
3061 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
3121 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
3181 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
3241 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
3301 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
3361 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
3421 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
3481 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
3541 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
3601 agcggccgcg actctagagt cgacctgcag gcatgcaagc ttgcaggcat gcaagcttga
3661 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
3721 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
3781 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
3841 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
3901 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
3961 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
4021 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
4081 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
4141 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
4201 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
4261 cccgcatcga taccgtcgtc cacggcagct gtagatctta gccacttttt aaaagaaaag
4321 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
4381 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
4441 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
4501 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
4561 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
4621 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
4681 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
4741 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
4801 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
4861 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
4921 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
4981 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
5041 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
5101 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
5161 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
5221 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
5281 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
5341 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
5401 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
5461 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
5521 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
5581 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
5641 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
5701 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
5761 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
5821 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
5881 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
5941 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
6001 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
6061 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
6121 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
6181 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
6241 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
6301 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
6361 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
6421 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
6481 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
6541 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
6601 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
6661 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
6721 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
6781 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
6841 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
6901 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
6961 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
7021 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
7081 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
7141 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
7201 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
7261 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
7321 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
7381 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
7441 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
7501 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
7561 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
7621 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
7681 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
7741 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
7801 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
7861 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
7921 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
7981 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
8041 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
8101 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
8161 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
8221 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
8281 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
8341 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
8401 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
8461 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
8521 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
8581 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
8641 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
8701 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
8761 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
8821 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
8881 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
8941 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
9001 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
9061 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
9121 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
9181 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
9241 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
9301 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
9361 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
9421 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
9481 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
9541 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
9601 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
9661 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
9721 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
9781 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
9841 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
9901 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
9961 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
10021 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
10081 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
10141 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
10201 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
10261 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
10321 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
10381 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
10441 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
10501 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
10561 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
10621 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
10681 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
10741 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
10801 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
10861 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
10921 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
10981 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
11041 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
11101 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
11161 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
11221 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
11281 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
11341 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
11401 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
11461 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
11521 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
11581 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
11641 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
11701 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
11761 ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta
11821 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
11881 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
11941 attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcggagcac
12001 tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct ccgaacgtcg
12061 gagcactgtc ctccgaacgg agcatgtcct ccgaacgtcg gagcactgtc ctccgaacga
12121 ctagttaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
12181 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
12241 agcctctcga cattcgttgg atcgccgcc
//
