pSFVCs-lacZ

LOCUS        pSFVCs-lacZ              14923 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  This is a high level expression plasmid for production of 
            recombinant genomic SFV RNA encoding a C-beta galactosidase fusion 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    pSFVCs-lacZ
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14923)
  AUTHORS   Sjoberg EM, Suomalainen M, Garoff H
  TITLE     A significantly improved Semliki Forest virus expression system 
            based on translation enhancer segments from the viral capsid gene.
  JOURNAL   Biotechnology (N Y). 1994 Nov;12(11):1127-31.
  PUBMED    7765556
REFERENCE   2  (bases 1 to 14923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14923
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             8145..11192
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(8147..8169)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12138..12155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12309..12897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12389..12408)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13068..13928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13691..13710
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13929..14033)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14101..14119
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14157..14179)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14279..14298
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14512..14531
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        14821..14839
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14821..14838
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atggccgcca aagtgcatgt tgatattgag gctgacagcc cattcatcaa gtctttgcag
       61 aaggcatttc cgtcgttcga ggtggagtca ttgcaggtca caccaaatga ccatgcaaat
      121 gccagagcat tttcgcacct ggctaccaaa ttgatcgagc aggagactga caaagacaca
      181 ctcatcttgg atatcggcag tgcgccttcc aggagaatga tgtctacgca caaataccac
      241 tgcgtatgcc ctatgcgcag cgcagaagac cccgaaaggc tcgtatgcta cgcaaagaaa
      301 ctggcagcgg cctccgggaa ggtgctggat agagagatcg caggaaaaat caccgacctg
      361 cagaccgtca tggctacgcc agacgctgaa tctcctacct tttgcctgca tacagacgtc
      421 acgtgtcgta cggcagccga agtggccgta taccaggacg tgtatgctgt acatgcacca
      481 acatcgctgt accatcaggc gatgaaaggt gtcagaacgg cgtattggat tgggtttgac
      541 accaccccgt ttatgtttga cgcgctagca ggcgcgtatc caacctacgc cacaaactgg
      601 gccgacgagc aggtgttaca ggccaggaac ataggactgt gtgcagcatc cttgactgag
      661 ggaagactcg gcaaactgtc cattctccgc aagaagcaat tgaaaccttg cgacacagtc
      721 atgttctcgg taggatctac attgtacact gagagcagaa agctactgag gagctggcac
      781 ttaccctccg tattccacct gaaaggtaaa caatccttta cctgtaggtg cgataccatc
      841 gtatcatgtg aagggtacgt agttaagaaa atcactatgt gccccggcct gtacggtaaa
      901 acggtagggt acgccgtgac gtatcacgcg gagggattcc tagtgtgcaa gaccacagac
      961 actgtcaaag gagaaagagt ctcattccct gtatgcacct acgtcccctc aaccatctgt
     1021 gatcaaatga ctggcatact agcgaccgac gtcacaccgg aggacgcaca gaagttgtta
     1081 gtgggattga atcagaggat agttgtgaac ggaagaacac agcgaaacac taacacgatg
     1141 aagaactatc tgcttccgat tgtggccgtc gcatttagca agtgggcgag ggaatacaag
     1201 gcagaccttg atgatgaaaa acctctgggt gtccgagaga ggtcacttac ttgctgctgc
     1261 ttgtgggcat ttaaaacgag gaagatgcac accatgtaca agaaaccaga cacccagaca
     1321 atagtgaagg tgccttcaga gtttaactcg ttcgtcatcc cgagcctatg gtctacaggc
     1381 ctcgcaatcc cagtcagatc acgcattaag atgcttttgg ccaagaagac caagcgagag
     1441 ttaatacctg ttctcgacgc gtcgtcagcc agggatgctg aacaagagga gaaggagagg
     1501 ttggaggccg agctgactag agaagcctta ccacccctcg tccccatcgc gccggcggag
     1561 acgggagtcg tcgacgtcga cgttgaagaa ctagagtatc acgcaggtgc aggggtcgtg
     1621 gaaacacctc gcagcgcgtt gaaagtcacc gcacagccga acgacgtact actaggaaat
     1681 tacgtagttc tgtccccgca gaccgtgctc aagagctcca agttggcccc cgtgcaccct
     1741 ctagcagagc aggtgaaaat aataacacat aacgggaggg ccggccgtta ccaggtcgac
     1801 ggatatgacg gcagggtcct actaccatgt ggatcggcca ttccggtccc tgagtttcaa
     1861 gctttgagcg agagcgccac tatggtgtac aacgaaaggg agttcgtcaa caggaaacta
     1921 taccatattg ccgttcacgg accgtcgctg aacaccgacg aggagaacta cgagaaagtc
     1981 agagctgaaa gaactgacgc cgagtacgtg ttcgacgtag ataaaaaatg ctgcgtcaag
     2041 agagaggaag cgtcgggttt ggtgttggtg ggagagctaa ccaacccccc gttccatgaa
     2101 ttcgcctacg aagggctgaa gatcaggccg tcggcaccat ataagactac agtagtagga
     2161 gtctttgggg ttccgggatc aggcaagtct gctattatta agagcctcgt gaccaaacac
     2221 gatctggtca ccagcggcaa gaaggagaac tgccaggaaa tagtcaacga cgtgaagaag
     2281 caccgcggac tggacatcca ggcaaaaaca gtggactcca tcctgctaaa cgggtgtcgt
     2341 cgtgccgtgg acatcctata tgtggacgag gctttcgctt gccattccgg tactctgcta
     2401 gccctaattg ctcttgttaa acctcggagc aaagtggtgt tatgcggaga ccccaagcaa
     2461 tgcggattct tcaatatgat gcagcttaag gtgaacttca accacaacat ctgcactgaa
     2521 gtatgtcata aaagtatatc cagacgttgc acgcgtccag tcacggccat cgtgtctacg
     2581 ttgcactacg gaggcaagat gcgcacgacc aacccgtgca acaaacccat aatcatagac
     2641 accacaggac agaccaagcc caagccagga gacatcgtgt taacatgctt ccgaggctgg
     2701 gtaaagcagc tgcagttgga ctaccgtgga cacgaagtca tgacagcagc agcatctcag
     2761 ggcctcaccc gcaaaggggt atacgccgta aggcagaagg tgaatgaaaa tcccttgtat
     2821 gcccctgcgt cggagcacgt gaatgtactg ctgacgcgca ctgaggatag gctggtgtgg
     2881 aaaacgctgg ccggcgatcc ctggattaag gtcctatcaa acattccaca gggtaacttt
     2941 acggccacat tggaagaatg gcaagaagaa cacgacaaaa taatgaaggt gattgaagga
     3001 ccggctgcgc ctgtggacgc gttccagaac aaagcgaacg tgtgttgggc gaaaagcctg
     3061 gtgcctgtcc tggacactgc cggaatcaga ctcactgcag aagagtggag cacgattatt
     3121 acagcattta aggaggacag agcttactct ccagtggtgg ccttgaatga aatttgcacc
     3181 aagtactatg gagttgacct ggacagtggc ctgttttctg ccccgaaggt gtccctgtat
     3241 tacgagaaca accactggga taacagacct ggtggaagga tgtatggatt caatgccgca
     3301 acagctgcca ggctggaagc tagacatacc ttcctgaagg ggcagtggca tacgggcaag
     3361 caggcagtta tcgcagaaag aaaaatccaa ccgctttctg tgctggacaa tgtaattcct
     3421 atcaaccgca ggctgccgca cgccctggtg gctgagtaca agacggttaa aggcagtagg
     3481 gttgagtggc tggtcaataa agtaagaggg taccacgtcc tgctggtgag tgagtacaac
     3541 ctggctttgc ctcgacgcag ggtcacttgg ttgtcaccgc tgaatgtcac aggcgccgat
     3601 aggtgctacg acctaagttt aggactgccg gctgacgccg gcaggttcga cttggtcttt
     3661 gtgaacattc acacggaatt cagaatccac cactaccagc agtgtgtcga ccacgccatg
     3721 aagctgcaga tgcttggggg agatgcgcta cgactgctaa aacccggcgg cagcctcttg
     3781 atgagagctt acggatacgc cgataaaatc agcgaagccg ttgtttcctc cttaagcaga
     3841 aagttctcgt ctgcaagagt gttgcgcccg gattgtgtca ccagcaatac agaagtgttc
     3901 ttgctgttct ccaacttcga taacggaaag aggccctcaa ccttacacca gatgaatacc
     3961 aagctgagtg ccgtgtatgc cggagaagcc atgcacacgg ccgggtgtgc accatcctac
     4021 agagttaaga gagcagacat agccacgtgc acagaagcgg ctgtggttaa cgcagctaac
     4081 gcccgtggaa ctgtagggga tggcgtatgc agggccgtgg cgaagaaatg gccgtcagcc
     4141 tttaagggag aagcaacacc agtgggcaca attaaaacag tcatgtgcgg ctcgtacccc
     4201 gtcatccacg ctgtagcgcc taatttctct gccacgactg aagcggaagg ggaccgcgaa
     4261 ttggccgctg tctaccgggc agtggccgcc gaagtaaaca gactgtcact gagcagcgta
     4321 gccatcccgc tgctgtccac aggagtgttc agcggcggaa gagataggct gcagcaatcc
     4381 ctcaaccatc tattcacagc aatggacgcc acggacgctg acgtgaccat ctactgcaga
     4441 gacaaaagtt gggagaagaa aatccaggaa gccatagaca tgaggacggc tgtggagttg
     4501 ctcaatgatg acgtggagct gaccacagac ttggtgagag tgcacccgga cagcagcctg
     4561 gtgggtcgta agggctacag taccactgac gggtcgctgt actcgtactt tgaaggtacg
     4621 aaattcaacc aggctgctat tgatatggca gagatactga cgttgtggcc cagactgcaa
     4681 gaggcaaacg aacagatatg cctatacgcg ctgggcgaaa caatggacaa catcagatcc
     4741 aaatgtccgg tgaacgattc cgattcatca acacctccca ggacagtgcc ctgcctgtgc
     4801 cgctacgcaa tgacagcaga acggatcgcc cgccttaggt cacaccaagt taaaagcatg
     4861 gtggtttgct catcttttcc cctcccgaaa taccatgtag atggggtgca gaaggtaaag
     4921 tgcgagaagg ttctcctgtt cgacccgacg gtaccttcag tggttagtcc gcggaagtat
     4981 gccgcatcta cgacggacca ctcagatcgg tcgttacgag ggtttgactt ggactggacc
     5041 accgactcgt cttccactgc cagcgatacc atgtcgctac ccagtttgca gtcgtgtgac
     5101 atcgactcga tctacgagcc aatggctccc atagtagtga cggctgacgt acaccctgaa
     5161 cccgcaggca tcgcggacct ggcggcagat gtgcatcctg aacccgcaga ccatgtggac
     5221 ctcgagaacc cgattcctcc accgcgcccg aagagagctg cataccttgc ctcccgcgcg
     5281 gcggagcgac cggtgccggc gccgagaaag ccgacgcctg ccccaaggac tgcgtttagg
     5341 aacaagctgc ctttgacgtt cggcgacttt gacgagcacg aggtcgatgc gttggcctcc
     5401 gggattactt tcggagactt cgacgacgtc ctgcgactag gccgcgcggg tgcatatatt
     5461 ttctcctcgg acactggcag cggacattta caacaaaaat ccgttaggca gcacaatctc
     5521 cagtgcgcac aactggatgc ggtcgaggag gagaaaatgt acccgccaaa attggatact
     5581 gagagggaga agctgttgct gctgaaaatg cagatgcacc catcggaggc taataagagt
     5641 cgataccagt ctcgcaaagt ggagaacatg aaagccacgg tggtggacag gctcacatcg
     5701 ggggccagat tgtacacggg agcggacgta ggccgcatac caacatacgc ggttcggtac
     5761 ccccgccccg tgtactcccc taccgtgatc gaaagattct caagccccga tgtagcaatc
     5821 gcagcgtgca acgaatacct atccagaaat tacccaacag tggcgtcgta ccagataaca
     5881 gatgaatacg acgcatactt ggacatggtt gacgggtcgg atagttgctt ggacagagcg
     5941 acattctgcc cggcgaagct ccggtgctac ccgaaacatc atgcgtacca ccagccgact
     6001 gtacgcagtg ccgtcccgtc accctttcag aacacactac agaacgtgct agcggccgcc
     6061 accaagagaa actgcaacgt cacgcaaatg cgagaactac ccaccatgga ctcggcagtg
     6121 ttcaacgtgg agtgcttcaa gcgctatgcc tgctccggag aatattggga agaatatgct
     6181 aaacaaccta tccggataac cactgagaac atcactacct atgtgaccaa attgaaaggc
     6241 ccgaaagctg ctgccttgtt cgctaagacc cacaacttgg ttccgctgca ggaggttccc
     6301 atggacagat tcacggtcga catgaaacga gatgtcaaag tcactccagg gacgaaacac
     6361 acagaggaaa gacccaaagt ccaggtaatt caagcagcgg agccattggc gaccgcttac
     6421 ctgtgcggca tccacaggga attagtaagg agactaaatg ctgtgttacg ccctaacgtg
     6481 cacacattgt ttgatatgtc ggccgaagac tttgacgcga tcatcgcctc tcacttccac
     6541 ccaggagacc cggttctaga gacggacatt gcatcattcg acaaaagcca ggacgactcc
     6601 ttggctctta caggtttaat gatcctcgaa gatctagggg tggatcagta cctgctggac
     6661 ttgatcgagg cagcctttgg ggaaatatcc agctgtcacc taccaactgg cacgcgcttc
     6721 aagttcggag ctatgatgaa atcgggcatg tttctgactt tgtttattaa cactgttttg
     6781 aacatcacca tagcaagcag ggtactggag cagagactca ctgactccgc ctgtgcggcc
     6841 ttcatcggcg acgacaacat cgttcacgga gtgatctccg acaagctgat ggcggagagg
     6901 tgcgcgtcgt gggtcaacat ggaggtgaag atcattgacg ctgtcatggg cgaaaaaccc
     6961 ccatattttt gtgggggatt catagttttt gacagcgtca cacagaccgc ctgccgtgtt
     7021 tcagacccac ttaagcgcct gttcaagttg ggtaagccgc taacagctga agacaagcag
     7081 gacgaagaca ggcgacgagc actgagtgac gaggttagca agtggttccg gacaggcttg
     7141 ggggccgaac tggaggtggc actaacatct aggtatgagg tagagggctg caaaagtatc
     7201 ctcatagcca tggccacctt ggcgagggac attaaggcgt ttaagaaatt gagaggacct
     7261 gttatacacc tctacggcgg tcctagattg gtgcgttaat acacagaatt ctgattatag
     7321 cgcactatta tagcaccatg aattacatcc ctacgcaaac gttttacggc cgccggtggc
     7381 gcccgcgccc ggcggcccgt ccctggccgt tgcaggccac tccggtggct cccgtcgtcc
     7441 ccgacttcca ggcccagcag atgcagcaac tcatcagcgc cgtaaatgcg ctgacaatga
     7501 gacagaacgc aattgctcct gctaggcctc ccaaaccaaa gaagaagaag acaaccaaac
     7561 caaagccgaa aacgcagccc aagaagatca acggaaaaac gcagcagcaa aagaagaaag
     7621 acaagcaagc cgacaagaag aagaagaaac ccggaaaaag agaaagaatg tgcatgaaga
     7681 ttgaaaatga ctgtatcttc gaagtcaaac acgaaggaaa ggtcactggg tacgcctgcc
     7741 tggtgggcga caaagtcatg aaacctgccc acgtgaaagg agtcatcgac aacgcggacc
     7801 tggcaaagct agctttcaag aaatcgagca agtatgacct tgagtgtgcc cagataccag
     7861 ttcacatgag gtcggatgcc tcaaagtaca cgcatgagaa gcccgaggga cactataact
     7921 ggcaccacgg ggctgttcag tacagcggag gtaggttcac tataccgaca ggagcgggca
     7981 aaccgggaga cagtggcagg cccatattcg ataacaaggg gagggtagtc gcaatcgtcc
     8041 tgggtggggc caacgagggc tcacgcacag cactgtcggt ggtcacctgg aacaaagata
     8101 tggtgactag agtgaccccc gaggggtccg aagagtggga tcccgtcgtt ttacaacgtc
     8161 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8221 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8281 tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc
     8341 tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg
     8401 gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg
     8461 ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc
     8521 tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt
     8581 gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga
     8641 gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg
     8701 gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt
     8761 tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg
     8821 atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc
     8881 tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt
     8941 tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga
     9001 acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg
     9061 aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg
     9121 aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg
     9181 ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc
     9241 aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga
     9301 accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca
     9361 atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac
     9421 cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga
     9481 tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct
     9541 ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca
     9601 cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg
     9661 ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga
     9721 tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc
     9781 aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt
     9841 cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg
     9901 atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc
     9961 atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc
    10021 aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact
    10081 ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc
    10141 cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac
    10201 tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca
    10261 tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt
    10321 cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata
    10381 agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa
    10441 aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg
    10501 gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg
    10561 gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg
    10621 tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga
    10681 aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga
    10741 gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc
    10801 gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct
    10861 gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg
    10921 aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg
    10981 acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc
    11041 atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg
    11101 gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct
    11161 accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggggg gatcccgggt
    11221 aattaattga attacatccc tacgcaaacg ttttacggcc gccggtggcg cccgcgcccg
    11281 gcggcccgtc cctggccgtt gcaggccact ccggtggctc ccgtcgtccc cgacttccag
    11341 gcccagcaga tgcagcaact catcagcgcc gtaaatgcgc tgacaatgag acagaacgca
    11401 attgctcctg ctaggcctcc caaaccaaag aagaagaaga caaccaaacc aaagccgaaa
    11461 acgcagccca agaagatcaa cggaaaaacg cagcagcaaa agaagaaaga caagcaagcc
    11521 gacaagaaga agaagaaacc cggaaaaaga gaaagaatgt gcatgaagat tgaaaatgac
    11581 tgtatctatg cggctagcca cagtaacgta gtgtttccag acatgtcggg caccgcacta
    11641 tcatgggtgc agaaaatctc gggtggtctg ggggccttcg caatcggcgc tatcctggtg
    11701 ctggttgtgg tcacttgcat tgggctccgc agataagtta gggtaggcaa tggcattgat
    11761 atagcaagaa aattgaaaac agaaaaagtt agggtaagca atggcatata accataactg
    11821 tataacttgt aacaaagcgc aacaagacct gcgcaattgg ccccgtggtc cgcctcacgg
    11881 aaactcgggg caactcatat tgacacatta attggcaata attggaagct tacataagct
    11941 taattcgacg aataattgga tttttatttt attttgcaat tggtttttaa tatttccaaa
    12001 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
    12061 aaaaaaacta gtctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    12121 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    12181 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    12241 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    12301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    12361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    12421 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    12481 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    12541 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    12601 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    12661 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    12721 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    12781 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    12841 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12901 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    12961 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    13021 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    13081 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    13141 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    13201 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    13261 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    13321 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    13381 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    13441 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    13501 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    13561 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    13621 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    13681 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    13741 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    13801 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    13861 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    13921 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    13981 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    14041 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    14101 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    14161 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    14221 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
    14281 gcatcagagc agattgtact gagagtgcac cattcgacgc tctcccttat gcgactcctg
    14341 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
    14401 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
    14461 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
    14521 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
    14581 ggcgtagagg atctggctag cgatgaccct gctgattggt tcgctgacca tttccgggtg
    14641 cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt
    14701 ttacgagaga gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta
    14761 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
    14821 atttaggtga cactatagat ggcggatgtg tgacatacac gacgccaaaa gattttgttc
    14881 cagctcctgc cacctccgct acgcgagaga ttaaccaccc acg
//