| 质粒名称: | pPBbsr2-4031NES |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 105241 |
| 存储实验室: | Michiyuki Matsuda |
|
载体骨架基本信息 |
|
| 载体名称: | pPB |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6800 bp |
| 完整质粒大小: | 9116 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 杀稻瘟菌素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | AMPK-EV |
| 物种来源: | S. cerevisiae (budding yeast), Synthetic; aequorea coerulescens |
| 插入大小: | 2300 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CAGGS |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | SalI（在克隆过程中被销毁） |
| 5' 测序引物 | pCAGGS-5' |
| 3' 测序引物 | IRES-reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pPBbsr2-4031NES was a gift from Michiyuki Matsuda (Addgene plasmid # 105241 ; http://n2t.net/addgene:105241 ; RRID:Addgene_105241) |
| 参考文献部分: | A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs. Konagaya Y, Terai K, Hirao Y, Takakura K, Imajo M, Kamioka Y, Sasaoka N, Kakizuka A, Sumiyama K, Asano T, Matsuda M. Cell Rep. 2017 Nov 28;21(9):2628-2638. doi: 10.1016/j.celrep.2017.10.113. 10.1016/j.celrep.2017.10.113 PubMed 29186696 |
买家导航
pPBbsr24031NES载体基本信息
pPBbsr24031NES载体图谱质粒图谱和多克隆位点信息
pPBbsr24031NES载体简介
pPBbsr24031NES载体序列
LOCUS pPBbsr2-4031NES 9116 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION A Forster resonance energy transfer (FRET) biosensor for AMPK
activity with cyan and yellow fluorescent protein..
ACCESSION .
VERSION .
KEYWORDS pPBbsr2-4031NES
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9116)
AUTHORS Konagaya Y, Terai K, Hirao Y, Takakura K, Imajo M, Kamioka Y,
Sasaoka N, Kakizuka A, Sumiyama K, Asano T, Matsuda M
TITLE A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous
AMPK Responses among Cells and Organs.
JOURNAL Cell Rep. 2017 Nov 28;21(9):2628-2638. doi:
10.1016/j.celrep.2017.10.113.
PUBMED 29186696
REFERENCE 2 (bases 1 to 9116)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9116
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(140..161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 352..371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
repeat_region 674..708
/label=piggyBac left (5') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
enhancer 1011..1390
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1393..1668
/label=chicken beta-actin promoter
intron 1669..2677
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2601..2623
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
CDS 2735..3253
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIE"
primer_bind 4975..4996
/label=EGFP-C
/note="EGFP, forward primer"
CDS 5045..5071
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
primer_bind complement(5325..5342)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5552..5571
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind complement(6200..6217)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6206..6430
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6477..6493)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6501..6517
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6525..6555)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6570..6591
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
repeat_region 6825..6887
/label=piggyBac right (3') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
primer_bind complement(6928..6948)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(6928..6946)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6967..6983)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6991..7007
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7015..7045)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7060..7081
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7198..7215)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7369..7957)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7449..7468)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8128..8988)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8751..8770
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8989..9093)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
1201 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
1381 tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
1681 ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg
1741 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
1801 cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc
1861 cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
1921 ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
1981 gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
2041 gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
2101 agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct ccccgagttg
2161 ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg gggctcgccg
2221 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
2281 gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc gaggcgcggc
2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
2401 ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
2461 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
2521 gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg gctgccttcg
2581 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
2641 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt
2701 tgtgctgtct catcattttg gcaaagaatt cggcatggtg agcaagggcg aggagctgtt
2761 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
2821 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agcttctatg
2881 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
2941 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
3001 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
3061 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
3121 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
3181 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
3241 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
3301 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgtt
3361 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcctga ccgccgccgg
3421 gatcactgag ggcatgaacg agctgtacct cgagaagttt tctcaagaac agatcggcga
3481 aaacattgtg tgcagggtca tttgtaccac gggtcaaatt cccatccgag atttgtcagc
3541 tgatatttca caagtgctta aggaaaaacg atccataaag aaagtttgga catttggtag
3601 aaacccagcc tgtgactatc atttaggaaa catttcaaga ctgtcaaata agcatttcca
3661 aatactacta ggagaagacg gtaacctttt attgaatgac atttccacta atgggacctg
3721 gttaaatggg caaaaagtcg agaagaacag caatcagtta ctgtcccaag gtgatgaaat
3781 aaccgttggt gtaggcgtgg aatcagatat tttatctctg gtcattttca taaacgacaa
3841 atttaagcag tgcctggagc agaacaaagt tgatcgcggt accagtgctg gtggtagtgc
3901 tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca gtgctggtgg
3961 tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
4021 tggtggtagt gctggtggtt ccggcagtgc tggtggtagt gctggtggta gtaccagtgc
4081 tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggttccgg
4141 cagtgctggt ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg gtggtagtgc
4201 tggtggtagt gctggtggta gtgctggtgg ttccggaggc agcggcgagg gcagcaccaa
4261 gatgaggagg gtggccaccc tggtggacct gggcaccggc ggcagcgagc tgggcggccg
4321 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
4381 cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg atgccaccta
4441 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
4501 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
4561 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gtaccatctt
4621 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
4681 ggtgaaccgc atcgagctga agggcatcgg cttcaaggag gacggcaaca tcctggggca
4741 caagctagag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
4801 cggcatcaag gcccacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
4861 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
4921 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
4981 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtctagact
5041 gcagctgcct cctctggaac gcctgactct ggattaagtc gagggccggc gcgccgcggc
5101 cggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc tagcgggatc
5161 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
5221 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
5281 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
5341 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
5401 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
5461 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
5521 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
5581 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
5641 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
5701 gtggttttcc tttgaaaaac acgataatac catggtcatg aaaacattta acatttctca
5761 acaagatcta gaattagtag aagtagcgac agagaagatt acaatgcttt atgaggataa
5821 taaacatcat gtgggagcgg caattcgtac gaaaacagga gaaatcattt cggcagtaca
5881 tattgaagcg tatataggac gagtaactgt ttgtgcagaa gccattgcga ttggtagtgc
5941 agtttcgaat ggacaaaagg attttgacac gattgtagct gttagacacc cttattctga
6001 cgaagtagat agaagtattc gagtggtaag tccttgtggt atgtgtaggg agttgatttc
6061 agactatgca ccagattgtt ttgtgttaat agaaatgaat ggcaagttag tcaaaactac
6121 gattgaagaa ctcattccac tcaaatatac ccgaaattaa aagtcgagat gcatcgatga
6181 tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
6241 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
6301 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
6361 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
6421 ggctctatgg cttctgaggc ggaaagaacc tgcagcccaa gcttggcgta atcatggtca
6481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
6541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
6601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatctt aaaagttttg
6661 ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat
6721 aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct
6781 attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac
6841 gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta atctagtata
6901 cgcgtttaat taatccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
6961 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
7021 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
7081 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
7141 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
7201 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
7261 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
7321 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
7381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
7441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
7501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
7561 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
7621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
7681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
7741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
7801 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
7861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
7921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
7981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
8041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
8101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
8161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
8221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
8281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
8341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
8401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
8461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
8521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
8581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
8641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
8701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
8761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
8821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
8881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
8941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
9001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
9061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
