| 质粒名称: | pCMV-PE7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 214812 |
| 存储实验室: | Brittany Adamson |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | Clontech |
| 空载体大小: | 3402bp |
| 完整质粒大小: | 10473bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PE7、PEmax-La(SSB)1-194 |
| 物种来源: | Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus; La(SSB)1-194 is from Homo sapiens |
| 插入大小: | 7071 bp |
| 启动子: | CMV |
| 标签/融合蛋白: |
SV40 bpNLS (N terminal on insert)
SV40 bpNLS (C terminal on insert)
c-Myc NLS (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BsrGI (not destroyed) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CMV Forward, T7 |
| 3’测序引物 | BGHR |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-PE7 was a gift from Brittany Adamson (Addgene plasmid # 214812 ; http://n2t.net/addgene:214812 ; RRID:Addgene_214812) |
| 参考文献部分: | Improving prime editing with an endogenous small RNA-binding protein. Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B. Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6. 10.1038/s41586-024-07259-6 PubMed 38570691 |
买家导航
pCMV-PE7载体质粒基本信息
pCMV-PE7质粒图谱载体图谱和pCMV-PE7载体序列质粒序列多克隆位点信息
pCMV-PE7质粒载体简介
pCMV-PE7质粒序列载体序列
LOCUS pCMV-PE7 10473 bp DNA circular SYN 14-APR-2025
DEFINITION Mammalian expression of SpCas9 PE7 prime editor.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10473)
AUTHORS Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y,
Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC,
Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B
TITLE Improving prime editing with an endogenous small RNA-binding
protein.
JOURNAL Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6.
PUBMED 38570691
REFERENCE 2 (bases 1 to 10473)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature.
2024 Apr 3. doi: 10.1038/s41586-024-07259-6."
COMMENT Sequence Label: pCMV-PE7
FEATURES Location/Qualifiers
source 1..10473
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(join(171..962,963..1031))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 794..813
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1032..1136)
/gene="bla"
/label=AmpR promoter
primer_bind complement(1215..1234)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 1406..1785
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1786..1989
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1940..1960
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2031..2050
/label=T7
/note="T7 promoter, forward primer"
promoter 2031..2049
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2099..2119
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2120..6220
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6278..6298
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 9077..9097
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 9107..9133
/codon_start=1
/product="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
/label=c-myc NLS
/translation="PAAKRVKLD"
CDS 9142..9159
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(9182..9199)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9188..9412
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(9483..9499)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9483..9499)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9496..9518)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9507..9523
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(9531..9537,9538..9555,9556..9561))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9576..9597
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9714..9731)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9885..10473)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9965..9984)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
2041 cactataggg agagccgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
2101 aaagaagaag cggaaagtcg acaagaagta cagcatcggc ctggacatcg gcaccaactc
2161 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
2221 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
2281 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
2341 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
2401 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
2461 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
2521 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
2581 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
2641 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
2701 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
2761 actgagcaag agcagaaagc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
2821 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
2881 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
2941 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
3001 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
3061 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
3121 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
3181 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
3241 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaagag
3301 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
3361 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
3421 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
3481 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
3541 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
3601 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
3661 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
3721 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
3781 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
3841 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
3901 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
3961 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
4021 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
4081 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
4141 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
4201 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
4261 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
4321 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
4381 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
4441 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
4501 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
4561 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
4621 ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
4681 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
4741 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
4801 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
4861 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
4921 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
4981 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
5041 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
5101 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
5161 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
5221 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
5281 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
5341 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
5401 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
5461 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
5521 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
5581 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
5641 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
5701 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
5761 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
5821 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
5881 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
5941 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
6001 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
6061 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
6121 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
6181 gtacgagaca cggatcgacc tgtctcagct gggaggtgac tccggcggaa gctctggtgg
6241 cagcaagcgg accgccgacg gctctgaatt cgagagccct aagaagaaaa gaaaggtgag
6301 cggaggctct agcggcggaa gcaccctgaa cattgaagac gagtatagac tgcatgaaac
6361 aagcaaggaa cccgacgtgt ccctgggctc cacctggctg tccgactttc cccaggcctg
6421 ggccgagaca ggaggaatgg gcctggccgt gcggcaggca cccctgatca tccctctgaa
6481 ggccacctct acacccgtga gcatcaagca gtaccctatg tctcaggagg ccagactggg
6541 catcaagcct cacatccaga ggctgctgga ccagggcatc ctggtgccat gccagagccc
6601 ctggaacaca ccactgctgc ccgtgaagaa gccaggcacc aatgactata gacccgtgca
6661 ggatctgaga gaggtgaaca agagggtgga ggatatccac cccaccgtgc ccaaccctta
6721 caatctgctg tccggcctgc ccccttctca ccagtggtat acagtgctgg acctgaagga
6781 tgccttcttt tgtctgagac tgcaccctac cagccagcca ctgttcgcct ttgagtggag
6841 ggaccctgag atgggcatct ctggccagct gacctggaca cgcctgcctc agggcttcaa
6901 gaatagccca acactgttta acgaggccct gcaccgcgac ctggcagatt tccggatcca
6961 gcacccagat ctgatcctgc tgcagtacgt ggacgatctg ctgctggccg ccaccagcga
7021 gctggattgc cagcagggaa cacgcgccct gctgcagacc ctgggaaacc tgggatatag
7081 ggcatccgcc aagaaggccc agatctgtca gaagcaggtg aagtacctgg gctatctgct
7141 gaaggagggc cagagatggc tgacagaggc caggaaggag acagtgatgg gccagccaac
7201 acccaagacc ccaagacagc tgagggagtt cctgggcaaa gcaggatttt gcaggctgtt
7261 catcccagga ttcgcagaga tggcagcacc tctgtaccca ctgaccaagc cgggcaccct
7321 gtttaattgg ggccctgacc agcagaaggc ctatcaggag atcaagcagg ccctgctgac
7381 agcaccagcc ctgggcctgc cagacctgac caagcctttc gagctgtttg tggatgagaa
7441 gcagggctac gccaagggcg tgctgaccca gaagctggga ccatggagac ggcccgtggc
7501 ctatctgtcc aagaagctgg acccagtggc agcaggatgg ccaccatgcc tgaggatggt
7561 ggcagcaatc gccgtgctga caaaggatgc cggcaagctg accatgggac agccactggt
7621 catcctggca ccacacgcag tggaggccct ggtgaagcag cctccagatc gctggctgtc
7681 taacgcccgg atgacacact accaggccct gctgctggac accgatcgcg tgcagtttgg
7741 ccctgtggtg gccctgaatc cagccaccct gctgcctctg ccagaggagg gcctgcagca
7801 caactgtctg gacatcctgg cagaggcaca cggaacaagg ccagacctga ccgatcagcc
7861 cctgcctgac gccgatcaca catggtatac cgatggaagc tccctgctgc aggagggcca
7921 gaggaaggca ggagcagcag tgaccacaga gacagaagtg atctgggcca aggccctgcc
7981 agcaggcaca tccgcccagc gggccgagct gatcgccctg acccaggccc tgaagatggc
8041 cgagggcaag aagctgaacg tgtacacaga ctccagatat gccttcgcca ccgcacacat
8101 ccacggagag atctacaggc gccggggctg gctgacctct gagggcaagg agatcaagaa
8161 caaggatgag atcctggccc tgctgaaggc cctgtttctg cccaagcggc tgagcatcat
8221 ccactgtcct ggacaccaga agggacactc cgccgaggca aggggcaatc ggatggccga
8281 ccaggccgcc agaaaggctg ctattactga aactcccgac acttccactc tgctgattga
8341 aaactcctcc ccttccggag gatctagcgg aggctcctct ggctctgaga cacctggcac
8401 aagcgagagc gcaacacctg aaagcagcgg gggcagcagc ggggggtcaa tggctgaaaa
8461 tggtgataat gaaaagatgg ctgccctgga ggccaaaatc tgtcatcaaa ttgagtatta
8521 ttttggcgac ttcaatttgc cacgggacaa gtttctaaag gaacagataa aactggatga
8581 aggctgggta cctttggaga taatgataaa attcaacagg ttgaaccgtc taacaacaga
8641 ctttaatgta attgtggaag cattgagcaa atccaaggca gaactcatgg aaatcagtga
8701 agataaaact aaaatcagaa ggtctccaag caaaccccta cctgaagtga ctgatgagta
8761 taaaaatgat gtaaaaaaca gatctgttta tattaaaggc ttcccaactg atgcaactct
8821 tgatgacata aaagaatggt tagaagataa aggtcaagta ctaaatattc agatgagaag
8881 aacattgcat aaagcattta agggatcaat ttttgttgtg tttgatagca ttgaatctgc
8941 taagaaattt gtagagaccc ctggccagaa gtacaaagaa acagacctgc taatactttt
9001 caaggacgat tactttgcca aaaaaaatga atctggcggc tcaaaaagaa ccgccgacgg
9061 cagcgaattc gagtctccca agaagaagag gaaagtcggc tctggccctg ccgctaagag
9121 agtgaagctg gactaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
9181 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
9241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagaa aattgcatcg
9301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
9361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
9421 gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca
9481 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
9541 gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt
9601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
9661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
9721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
9781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
9841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
9901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
9961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
10021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
10081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
10141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
10201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
10261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
10321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
10381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
10441 cagcagatta cgcgcagaaa aaaaggatct caa
//
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pCMV-PE7质粒载体应用举例
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