pLZ360

LOCUS       pLZ360              8787 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232
                     /label=CMV
     misc_feature    234..1300
                     /label=P_EF1a
     intron          353..1291
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     promoter        1309..1313
                     /label=P_TAL
     misc_recomb     1329..1362
                     /label=LoxP
     protein_bind    complement(1329..1362)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1370..1396
                     /label=192-WKG-615-F
     polyA_signal    1418..1642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      1651..1835
                     /label=SV40 late polyA
     misc_feature    1872..1905
                     /label=loxP
     protein_bind    complement(1872..1905)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1912..1960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1980..1989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1986..2048
                     /label=Igk secretion signal
     CDS             1986
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation=""
     CDS             1989
                     /codon_start=1
                     /label=mCherry
                     /translation=""
     CDS             2054
                     /codon_start=1
                     /label=linker
                     /translation=""
     CDS             2055..2753
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    2760..3503
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     2763..2784
                     /label=193-WKG-615-F
     primer_bind     complement(2827..2849)
                     /label=186-WKG-603-R
     misc_feature    3033..3035
                     /label=F93A
                     /note="F93A"
     misc_feature    3487..3503
                     /label=EpoR-D1_D2_TM
     misc_feature    3513..4341
                     /label=IL6ST
     misc_feature    3540..3566
                     /label=box1
     misc_feature    3864..3866
                     /label=Y759A
     polyA_signal    4384..4608
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4654..5082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4668..4692)
                     /label=184-WKG-599-R
     primer_bind     4953..4972
                     /label=189-WKG-612-F
     promoter        5096..5425
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    5276..5353
                     /label=SV40 ORI
     CDS             5492..6286
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6460..6581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6626..6646)
                     /label=M13-rev
     primer_bind     complement(6630..6646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6652..6674)
                     /label=LacO
     protein_bind    6654..6670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6678..6708)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(6679..6708)
                     /label=lac
     protein_bind    6723..6744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6850..7790
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7791..8651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8652..8756)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(8693..8721)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgggaaag
      241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      361 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      421 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggccggccga agcagaaggc
     1321 tagcgcttat aacttcgtat agcatacatt atacgaagtt atcggcgcgc caatgctccg
     1381 aatgcatcag tgcccctgca gggctgatca gcctcgactg tgccttctag ttgccagcca
     1441 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1501 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1561 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     1621 ggggatgcgg tgggctctat ggcttctgag gataagatac attgatgagt ttggacaaac
     1681 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1741 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1801 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc gcttggtacc gtctttccag
     1861 tggttaatta aataacttcg tatagcatac attatacgaa gttatcgatc cgaacaaacg
     1921 acccaacacc cgtgcgtttt attctgtctt tttattgccg atcctcgagg aattcgccgg
     1981 ccaccatgga gaccgatacc ttattactgt gggtgcttct tctgtgggtt cccggtagca
     2041 ccggagacgg atccgacatc gagaagctgt gcaagaaggc ggaagaggag gccaaggagg
     2101 cccaggagaa ggccgacgag ctgagacaac gacaccccga ttcgcaggcg gcggaagacg
     2161 cggaggatct cgcaaatctg gctgtcgccg ccgtgctgac cgcttgtctg ctggcccagg
     2221 agcatcccaa cgccgatatc gctaagctgt gcatcaaggc cgcgagcgaa gctgcggagg
     2281 ccgcgtcaaa agccgccgag ctggcccaac ggcacccaga cagtcaagca gccagagatg
     2341 ctatcaaact cgcctctcaa gccgcccgcg ccgtgatcct ggccatcatg ctcgccgcag
     2401 agaatcccaa cgctgatatt gcaaagctat gtattaaggc ggccagcgaa gccgcagagg
     2461 ctgcctcgaa agctgcggaa cttgcacagc ggcaccccga ctcccaggcc gcccgagatg
     2521 caattaaact ggccagtcag gccgctgagg cggtggagag agccatctgg ctggccgccg
     2581 agaaccccaa tgccgatatc gccaaaaaat gcatcaaagc ggcctccgaa gcagccgagg
     2641 aggcctctaa agctgccgaa gaggctcaaa gacacccaga cagccagaag gcacgcgacg
     2701 agatcaagga ggcctctcag aaggccgagg aggtgaagga gagatgcaag agcgaattcg
     2761 caccttcacc cagcctcccg gaccccaagt ttgagagcaa agcggccctg ctggcatccc
     2821 ggggctccga agaacttctg tgcttcaccc aacgcttgga agacttggtg tgtttctggg
     2881 aggaagcggc gagctccggg atggacttca actacagctt ctcataccag ctcgagggtg
     2941 agtcacgaaa gtcatgtagc ctgcaccagg ctcccaccgt ccgcggctcc gtgcgtttct
     3001 ggtgttcact gccaacagcg gacacatcga gtgctgtgcc gctggagctg caggtgacgg
     3061 aggcgtccgg ttctcctcgc tatcaccgca tcatccatat caatgaagta gtgctcctgg
     3121 acgcccccgc ggggctgctg gcgcgccggg cagaagaggg cagccacgtg gtgctgcgct
     3181 ggctgccacc tcctggagca cctatgacca cccacatccg atatgaagtg gacgtgtcgg
     3241 caggcaaccg ggcaggaggg acacaaaggg tggaggtcct ggaaggccgc actgagtgtg
     3301 ttctgagcaa cctgcggggc gggacgcgct acaccttcgc tgttcgagcg cgcatggccg
     3361 agccgagctt cagcggattc tggagtgcct ggtctgagcc cgcgtcacta ctgaccgcta
     3421 gcgacctgga ccctctcatc ttgacgctgt ctctcattct ggtcctcatc tcgttgttgc
     3481 tgacggttct ggccctgctg tccgcagcag caaataagcg agacctaatt aaaaaacaca
     3541 tctggcctaa tgttccagat ccttcaaaga gtcatattgc ccagtggtca cctcacactc
     3601 ctccaaggca caattttaat tcaaaagatc aaatgtattc agatggcaat ttcactgatg
     3661 taagtgttgt ggaaatagaa gcaaatgaca aaaagccttt tccagaagat ctgaaatcat
     3721 tggacctgtt caaaaaggaa aaaattaata ctgaaggaca cagcagtggt attggggggt
     3781 cttcatgcat gtcatcttct aggccaagca tttctagcag tgatgaaaat gaatcttcac
     3841 aaaacacttc gagcactgtc caggcttcta ccgtggtaca cagtggctac agacaccaag
     3901 ttccgtcagt ccaagtcttc tcaagatccg agtctaccca gcccttgtta gattcagagg
     3961 agcggccaga agatctacaa ttagtagatc atgtagatgg cggtgatggt atttttccca
     4021 ggcaacagta cttcaaacag aactgcagtc agcatgaatc cagtccagat atttcacatt
     4081 ttgaaaggtc aaagcaagtt tcatcagtca atgaggaaga ttttgttaga cttaaacagc
     4141 agatttcaga tcatatttca caatcctgtg gatctgggca aatgaaaatg tttcaggaag
     4201 tttctgcagc agatgctttt ggtccaggta ctgagggaca agtagaaaga tttgaaacag
     4261 ttggcatgga ggctgcgact gatgaaggca tgcctaaaag ttacttacca cagactgtac
     4321 ggcaaggcgg ctacatgcct cagtaatcta gagggcccgt ttaaacccgc tgatcagcct
     4381 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4441 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4501 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4561 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4621 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5101 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5161 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5221 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5281 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5341 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5401 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5461 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5521 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5581 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5641 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5701 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5761 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5821 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5881 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5941 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6001 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6061 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6121 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6181 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6241 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6301 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6361 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6421 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6481 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6541 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6601 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6781 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7441 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8761 tttccccgaa aagtgccacc tgacgtc
//