pYES2

价格:800元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pYES2
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5857 bp
5' 测序引物及序列: T7: TAATACGACTCACTATAGGG
3' 测序引物及序列: CYC1 Terminator: GTGACATAACTAATTACATGATG
载体标签: /
载体抗性: 氨苄
筛选标记: URA3
备注: 利用半乳糖诱导蛋白在酿酒酵母中表达。
产品目录号: V825-20
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYES2载体图谱


pYES2多克隆位点

载体简介

pYES2的是一个5.9 kb的载体,设计用来在酿酒酵母(Saccharomyces cerevisiae)中诱导表达重组蛋白。载体的特点在于基因插入载体的构建简单,以及能够使用原养型尿嘧啶进行转化株的筛选。

该载体包含以下元素:

1. 酵母GAL1启动子,能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白,同时能够被葡萄糖抑制表达

2.多克隆位点可以使用的很多限制酶切位点,便于基因插入。

3.CYC1终止子能够有效终止mRNA的转录。

4.能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。

5.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。

载体序列

LOCUS       pYES2	5857 bp 	DNA     circular	SYN
DEFINITION  pYES2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="pYES2"
                     /mol_type="other DNA"
     terminator      complement(12..251)
                     /label="CYC1_terminator"
     misc_feature    218..236
                     /label="pYESTrp_rev_primer"
     misc_feature    218..236
                     /label="CYC1_primer"
     promoter        complement(368..386)
                     /label="T7_promoter"
     promoter        complement(410..861)
                     /label="GAL1_promoter"
     misc_feature    complement(424..447)
                     /label="GAL1_primer"
     rep_origin      complement(1402..2873)
                     /label="2micron_origin"
     rep_origin      1404..2283
                     /label="2micron2_origin"
     promoter        2876..3101
                     /label="URA3_promoter"
     gene            3103..3903
                     /label="URA3"
                     /gene="URA3"
     CDS             3103..3906
                     /label="ORF frame 1"
     CDS             complement(3138..3614)
                     /label="ORF frame 3"
     gene            4002..4862
                     /label="Ampicillin (variant)"
                     /gene="Ampicillin (variant)"
     CDS             4002..4625
                     /label="ORF frame 3"
     rep_origin      5017..5636
                     /label="pBR322_origin"
ORIGIN
    1 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
   61 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
  121 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
  181 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA
  241 CTAATTACAT GATGCGGCCC TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT
  301 CTGCAGAATT CCAGCACACT GGCGGCCGTT ACTAGTGGAT CCGAGCTCGG TACCAAGCTT
  361 AATATTCCCT ATAGTGAGTC GTATTACAGC TGCTAGTAGT CCGATCCGGG GTTTTTTCTC
  421 CTTGACGTTA AAGTATAGAG GTATATTAAC AATTTTTTGT TGATACTTTT ATTACATTTG
  481 AATAAGAAGT AATACAAACC GAAAATGTTG AAAGTATTAG TTAAAGTGGT TAATGCAGTT
  541 TTTGCATTTA TATATCTGTT AATAGATCAA AAATCATCGC TTCGCTGATT AATTACCCCA
  601 GAAATAAGGC TAAAAAACTA ATCGCATTAT CATCCTATGG TTGTTAATTT GATTCGTTCA
  661 TTTGAAGGTT TGTGGGGCCA GGTTACTGCC AATTTTTCCT CTTCATAACC ATAAAAGCTA
  721 GTATTGTAGA ATCTTTATTG TTCGGAGCAG TGCGGCGCGA GGCACATCTG CGTTTCAGGA
  781 ACGCGACCGG TGAGGACGAG GACGCACGGA GGAGAGTCTT CCTTCGGAGG GCTGTCACCC
  841 GCTCGGCGGC TTCTAATCCG TACTAGTGGA TCATCCCCAC GCGCCCTGTA GCGCCCCATT
  901 AAGCGCGGCG GGTGTGGTGG TTACGCCCAG CGTGACCCCT ACACTTCCCA CCGCCCTAGC
  961 CCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
 1021 AGCTCTAAAT GGGGGCATCC GTTTACCCTT CCGATTTACT GCTTTACGGC ACCTCGACCC
 1081 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACCCTTTT
 1141 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTGG AAACTGGAAC
 1201 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGG
 1261 CTATTCGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
 1321 AACGTTTACA ATTTAAATAT TTGCTTATAC AATCTTCCTG TTTTTGGGGC TTTTCTGATT
 1381 ATCAACCGGG GTGGAGCTTC CCATTGCGAA TACCGCTTCC ACAAACATTG CTCAAAAGTA
 1441 TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC CTACCCATCC ACCTTTCGCT
 1501 CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT GTTTATCTCT AGTATTACTC
 1561 TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG AATCGAAAAC AATACGAAAA
 1621 TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG AAGAAACCGT TCATAATTTT
 1681 CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC ACAAAGTATG CGCAATCCAC
 1741 ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA AAATGCACCC GCAGCTTCGC
 1801 TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT TATGGAAGAG AAAATAGACA
 1861 CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC AAAAAGTTAT CAAGAGACTG
 1921 CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA TGCTTTGTTA GAAAAATAGC
 1981 GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA GATTCTTTGT TGGTAAAATA
 2041 GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT GTTTGAAAAA
 2101 TTAGCGCTCT CGTCGCGTTG CATTTTTGTT TTACAAAAAT GAAGCACAGA TTCTTCGTTG
 2161 GTAAAATAGC GCTTTCGCGT TGCATTTCTG TTCTGTAAAA ATGCAGCTCA GATTCTTTGT
 2221 TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTCTACA AAATGAAGCA CAGATGCTTC
 2281 GTTAACAAAG ATATGCTATT GAAGTGCAAG ATGGAAACGC AGAAAATGAA CCGGGGATGC
 2341 GACGTGCAAG ATTACCTATG CAATAGATGC AATAGTTTCT CCAGGAACCG AAATACATAC
 2401 ATTGTCTTCC GTAAAGCGCT AGACTATATA TTATTATACA GGTTCAAATA TACTATCTGT
 2461 TTCAGGGAAA ACTCCCAGGT TCGGATGTTC AAAATTCAAT GATGGGTAAC AAGTACGATC
 2521 GTAAATCTGT AAAACAGTTT GTCGGATATT AGGCTGTATC TCCTCAAAGC GTATTCGAAT
 2581 ATCATTGAGA AGCTGCAGCG TCACATCGGA TAATAATGAT GGCAGCCATT GTAGAAGTGC
 2641 CTTTTGCATT TCTAGTCTCT TTCTCGGTCT AGCTAGTTTT ACTACATCGC GAAGATAGAA
 2701 TCTTAGATCA CACTGCCTTT GCTGAGCTGG ATCAATAGAG TAACAAAAGA GTGGTAAGGC
 2761 CTCGTTAAAG GACAAGGACC TGAGCGGAAG TGTATCGTAC AGTAGACGGA GTATCTAGTA
 2821 TAGTCTATAG TCCGTGGAAT TAATTCTCAT CTTTGACAGC TTATCATCGA TAAGCTAGCT
 2881 TTTCAATTCA ATTCATCATT TTTTTTTTAT TCTTTTTTTT GATTTCGGTT TCTTTGAAAT
 2941 TTTTTTGATT CGGTAATCTC CGAACAGAAG GAAGAACGAA GGAAGGAGCA CAGACTTAGA
 3001 TTGGTATATA TACGCATATG TAGTGTTGAA GAAACATGAA ATTGCCCAGT ATTCTTAACC
 3061 CAACTGCACA GAACAAAAAC CTGCAGGAAA CGAAGATAAA TCATGTCGAA AGCTACATAT
 3121 AAGGAACGTG CTGCTACTCA TCCTAGTCCT GTTGCTGCCA AGCTATTTAA TATCATGCAC
 3181 GAAAAGCAAA CAAACTTGTG TGCTTCATTG GATGTTCGTA CCACCAAGGA ATTACTGGAG
 3241 TTAGTTGAAG CATTAGGTCC CAAAATTTGT TTACTAAAAA CACATGTGGA TATCTTGACT
 3301 GATTTTTCCA TGGAGGGCAC AGTTAAGCCG CTAAAGGCAT TATCCGCCAA GTACAATTTT
 3361 TTACTCTTCG AAGACAGAAA ATTTGCTGAC ATTGGTAATA CAGTCAAATT GCAGTACTCT
 3421 GCGGGTGTAT ACAGAATAGC AGAATGGGCA GACATTACGA ATGCACACGG TGTGGTGGGC
 3481 CCAGGTATTG TTAGCGGTTT GAAGCAGGCG GCAGAAGAAG TAACAAAGGA ACCTAGAGGC
 3541 CTTTTGATGT TAGCAGAATT GTCATGCAAG GGCTCCCTAT CTACTGGAGA ATATACTAAG
 3601 GGTACTGTTG ACATTGCGAA GAGCGACAAA GATTTTGTTA TCGGCTTTAT TGCTCAAAGA
 3661 GACATGGGTG GAAGAGATGA AGGTTACGAT TGGTTGATTA TGACACCCGG TGTGGGTTTA
 3721 GATGACAAGG GAGACGCATT GGGTCAACAG TATAGAACCG TGGATGATGT GGTCTCTACA
 3781 GGATCTGACA TTATTATTGT TGGAAGAGGA CTATTTGCAA AGGGAAGGGA TGCTAAGGTA
 3841 GAGGGTGAAC GTTACAGAAA AGCAGGCTGG GAAGCATATT TGAGAAGATG CGGCCAGCAA
 3901 AACTAAAAAA CTGTATTATA AGTAAATGCA TGTATACTAA ACTCACAAAT TAGAGCTTCA
 3961 ATTTAATTAT ATCAGTTATT ACCCATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
 4021 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
 4081 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
 4141 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
 4201 TGAGCACTTT TAAAGTTCTG CTATGTGATA CACTATTATC CCGTATTGAC GCCGGGCAAG
 4261 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
 4321 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
 4381 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
 4441 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
 4501 TGAATGAAGC CATACCAAAC GACGAGAGTG ACACCACGAT GCCTGTAGCA ATGCCAACAA
 4561 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
 4621 ACTGAATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
 4681 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
 4741 TGGGGCCAGA TGGTAAGCGC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
 4801 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
 4861 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
 4921 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
 4981 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
 5041 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
 5101 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
 5161 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
 5221 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
 5281 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
 5341 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
 5401 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
 5461 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
 5521 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
 5581 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
 5641 TTTTACGGTT CCTGGGCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
 5701 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
 5761 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC
 5821 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAG
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng