| 质粒名称: | MTS-mCherry-GFP1-10-Hyg-N1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 91957 |
| 存储实验室: | Rong Li |
|
载体骨架基本信息 |
|
| 载体名称: | pDsRed-Monomer-Hyg-N1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MTS-mCherry-GFP1-10 |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SalI（销毁后未知） |
| 3' 克隆位点: | NotI（销毁后未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | MTS-mCherry-GFP1-10-Hyg-N1 was a gift from Rong Li (Addgene plasmid # 91957 ; http://n2t.net/addgene:91957 ; RRID:Addgene_91957) |
| 参考文献部分: | Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R. Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695. Epub 2017 Mar 1. 10.1038/nature21695 PubMed 28241148 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MTS-mCherry-GFP1-10-Hyg-N1 6655 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses mCherry-GFP1-10 in mammalian cell mitochondria.
ACCESSION .
VERSION .
KEYWORDS MTS-mCherry-GFP1-10-Hyg-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6655)
AUTHORS Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R
TITLE Cytosolic proteostasis through importing of misfolded proteins into
mitochondria.
JOURNAL Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695.
Epub 2017 Mar 1.
PUBMED 28241148
REFERENCE 2 (bases 1 to 6655)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6655
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 69..426
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(92..112)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 277..412
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 339..358
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 434..1459
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
polyA_signal 1576..1657
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1685..1703)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1771..1875
/gene="bla"
/label=AmpR promoter
CDS 1876..2736
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2094..2113)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2922..3510
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3411..3430
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 3685..3988
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3989..4192
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4143..4163
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 4497..4506
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 6008..6129
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 6099..6118
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(6136..6591)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6273..6294)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6485..6504
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 6618..67
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
61 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
121 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
181 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
241 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
301 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
361 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
421 tgcaaagatc gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
481 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
541 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
601 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
661 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
721 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
781 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
841 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
901 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
961 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
1021 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
1081 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
1141 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
1201 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
1261 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
1321 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
1381 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
1441 tccgagggca aaggaataga gttctagagg atcataatca gccataccac atttgtagag
1501 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
1561 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
1621 atcacaaatt tcacaaataa agcatttttt tcactgcctc gcgatccaca accatggcga
1681 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
1741 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
1801 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
1861 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
1921 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
1981 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
2041 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
2101 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
2161 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
2221 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
2281 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
2341 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
2401 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
2461 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
2521 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
2581 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
2641 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
2701 gctgagatag gtgcctcact gattaagcat tggtaaggcg cgccaggccc tgccatagcc
2761 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
2821 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
2881 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
2941 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
3001 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
3061 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
3121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
3181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
3241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
3301 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
3361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
3421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
3481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
3541 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
3601 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
3661 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
3721 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
3781 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
3841 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
3901 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
3961 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
4021 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
4081 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
4141 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
4201 gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg
4261 cagtcgacat ggcctccact cgtgtcctcg cctctcgcct ggcctcccgg atggctgctt
4321 ccgccaaggt tgcccgccct gctgtccgcg ttgctcaggt cagcaagcgc accatccaga
4381 ctggctcccc cctccagacc ctcaagcgca cccagatgac ctccatcgtc aacgccacca
4441 cccgccaggc tttccagaag cgcgcctact cttccggatc cggtgacggt ccggtcgcca
4501 ccatggtttc taaaggtgaa gaagataata tggctattat taaagaattt atgagattta
4561 aagttcatat ggaaggttct gttaatggtc atgaatttga aattgaaggt gaaggtgaag
4621 gtagaccata tgaaggtact caaactgcta aattgaaagt tactaaaggt ggtccattgc
4681 catttgcttg ggatattttg tctccacaat ttatgtatgg ttctaaagct tatgttaaac
4741 atccagctga tattccagat tatttgaaat tgtcttttcc agaaggtttt aaatgggaaa
4801 gagttatgaa ttttgaagat ggtggtgttg ttactgttac tcaagattct tctttgcaag
4861 atggtgaatt tatttataaa gttaaattga gaggtactaa ttttccatct gatggtccag
4921 ttatgcaaaa aaaaactatg ggttgggaag cttcttctga aagaatgtat ccagaagatg
4981 gtgctttgaa aggtgaaatt aaacaaagat tgaaattgaa agatggtggt cattatgatg
5041 ctgaagttaa aactacttat aaagctaaaa aaccagttca attgccaggt gcttataatg
5101 ttaatattaa attggatatt acttctcata atgaagatta tactattgtt gaacaatatg
5161 aaagagctga aggtagacat tctactggtg gtatggatga attgtataaa gaattcggtg
5221 gtggttctgg cggaggttca atgtctaaag gtgaagaatt gtttactggt gttgttccaa
5281 ttttggttga attggatggt gatgttaatg gtcataaatt ttctgttaga ggtgaaggtg
5341 aaggtgatgc tactattggt aaattgactt tgaaatttat ttgtactact ggtaaattgc
5401 cagttccatg gccaactttg gttactactt tgacttatgg tgttcaatgt ttttctagat
5461 atccagatca tatgaaacaa catgattttt ttaaatctgc tatgccagaa ggttatgttc
5521 aagaaagaac tatttctttt aaagatgatg gtaaatataa aactagagct gttgttaaat
5581 ttgaaggtga tactttggtt aatagaattg aattgaaagg tactgatttt aaagaagatg
5641 gtaatatttt gggtcataaa ttggaatata attttaattc tcataatgtt tatattactg
5701 ctgataaaca aaaaaatggt attaaagcta attttactgt tagacataat gttgaagatg
5761 gttctgttca attggctgat cattatcaac aaaatactcc aattggtgat ggtccagttt
5821 tgttgccaga taatcattat ttgtctactc aaactgtttt gtctaaagat ccaaatgaaa
5881 aataaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
5941 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
6001 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
6061 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
6121 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
6181 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
6241 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
6301 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
6361 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
6421 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
6481 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
6541 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
6601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattc
//
