| 出品人: | Irene Castano Navarro（2015） |
|---|---|
| 载体名称: | pYC48 |
| 质粒类型: | 酿酒酵母表达载体；Recipient integrative Vector；FLP/FRT系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 6024 bp |
| 5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
| 3' 测序引物及序列: | -- |
| 载体标签: | HA |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3，Nourseothricin |
| 克隆菌株: | DH10B感受态细胞 ,30°C |
| 宿主细胞（系）: | 出芽酵母（酿酒酵母）、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #63905 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYC48 6024 bp DNA SYN
DEFINITION pYC48
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6024
/organism="pYC48"
/mol_type="other DNA"
misc_feature complement(29..51)
/label="pGEX_3_primer"
promoter 196..409
/label="URA3_promoter"
gene 411..1211
/label="URA3"
/gene="URA3"
CDS 411..1214
/label="ORF frame 3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS complement(446..922)
/label="ORF frame 3"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature complement(1814..1962)
/label="lacZ_a"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature 1928..1950
/label="M13_pUC_fwd_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 1943..1959
/label="M13_forward20_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 1966..1984
/label="T7_promoter"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature 2017..2033
/label="pBluescriptSK_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
CDS complement(2830..3333)
/label="ORF frame 1"
/translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
PVVYVRM*"
CDS 2869..3441
/label="ORF frame 1"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(3763..3782)
/label="T3_promoter"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(3799..3817)
/label="M13_reverse_primer"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
misc_feature complement(3816..3838)
/label="M13_pUC_rev_primer"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
rep_origin complement(4190..4809)
/label="pBR322_origin"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
gene complement(4964..5824)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
CDS complement(4964..5824)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5866..5894)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctaTACC
2041 CATACGATGT TCCTGACTAT GCGGGCTATC CGTATGACGT CCCGGACTAT GCAGGcTCCT
2101 ATCCATATGA CGTTCCAGAT TACGCTGCTC AGTGCtgaaG ATcCGAAGTT CCTATACTTT
2161 CTAGAGAATA GGAACTTCgt gcgcttttga accacgtaaa gtgctgtaaa Ccagctctat
2221 tcaatgaggt aaatgataaa gtttataaga gaccaaaaaa ttcaaaaaaa aaattgaaaa
2281 attatattgg tatttatatg aaacaatagt tcagttcaaa gcgctagctt ttctcatctc
2341 aactaaccgg tattaggtgg gtatttattt aatatgggaa caagaaatgt gacaTtaatg
2401 ttcatgtttt tattttcatt atttattcta tatggtttgg gacttgatgt tatgtcttaa
2461 ttcatagaGA TCCgcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
2521 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
2581 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
2641 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
2701 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
2761 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
2821 gttctcacat cacatccgaa cataaacaac cgGATCgatc ctacaattAT GTCTACTACT
2881 TTGGATGATA CTGCTTATAG ATACAGAACT TCTGTTCCAG GTGATGCTGA AGCTATTGAA
2941 GCTTTGGATG GTTCTTTCAC TACCGATACT GTTTTTAGAG TTACTGCTAC TGGTGATGGT
3001 TTCACTTTGA GAGAAGTTCC AGTTGATCCA CCATTGACTA AGGTTTTCCC AGATGATGAA
3061 TCCGATGATG AATCCGATGC TGGTGAAGAT GGTGATCCAG ATTCTAGAAC TTTCGTTGCT
3121 TATGGTGATG ATGGTGATTT GGCTGGTTTC GTTGTTGTTT CTTATTCTGG TTGGAACAGA
3181 AGATTGACTG TTGAAGATAT TGAAGTTGCT CCAGAACATA GAGGTCATGG TGTTGGTAGA
3241 GCTTTGATGG GTTTGGCTAC TGAATTCGCC AGAGAAAGAG GTGCTGGTCA TTTGTGGTTG
3301 GAAGTTACCA ATGTTAATGC TCCAGCTATT CATGCTTATA GAAGAATGGG TTTCACTTTG
3361 TGTGGTTTGG ATACTGCTTT ATACGATGGT ACTGCTTCCG ATGGTGAACA AGCTTTGTAT
3421 ATGTCTATGC CATGTCCATA AtaacTCGAC cagtactgac aataaaaaga ttcttgtttt
3481 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
3541 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
3601 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
3661 tcgatactaa cgccgccatc cagtgTCGAt cgacGAAGTT CCTATACTTT CTAGAGAATA
3721 GGAACTTCGT Cctcgagggg gggcccggta ccagcttttg ttccctttag tgagggttaa
3781 ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
3841 caattccaca caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag
3901 tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
3961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
4021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
4081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
4141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcC GCGTTGCTGG
4201 CGTTTTTCCA TAGGCTCGGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
4261 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT CCCCCCTGGA AGCTCCCTCG
4321 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
4381 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
4441 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
4501 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
4561 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
4621 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
4681 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
4741 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4801 CTTTGATCTt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
4861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
4921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagTTACCAA TGCTTAATCA
4981 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTGCCCG
5041 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
5101 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
5161 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
5221 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
5281 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
5341 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGAAAAAA AGCGGTTAGC TCCTTCGGTC
5401 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
5461 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
5521 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
5581 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
5641 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5701 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
5761 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
5821 TCATactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
5881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
5941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
6001 ggcgtatcac gaggcccttt cgtc
//
