pYC48

价格:1200元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品人: Irene Castano Navarro(2015)
载体名称: pYC48
质粒类型: 酿酒酵母表达载体;Recipient integrative Vector;FLP/FRT系统载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 6024 bp
5' 测序引物及序列: M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’
3' 测序引物及序列: --
载体标签: HA
载体抗性: 氨苄青霉素
筛选标记: URA3,Nourseothricin
克隆菌株: DH10B感受态细胞 ,30°C
宿主细胞(系): 出芽酵母(酿酒酵母)、白色念珠菌
备注: --
产品目录号: #63905
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYC48载体图谱



pYC48多克隆位点

载体简介

酿酒酵母整合型载体还有: 

pYC44
pYC56   红色荧光标签
pYC54   黄色荧光标签
pYC52   青色荧光标签
pYC50   13XMyc标签
pYC48   HA标签
pYC46   3XFlag标签



载体序列

LOCUS       pYC48	6024 bp 	DNA	SYN
DEFINITION  pYC48
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="pYC48"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     promoter        196..409
                     /label="URA3_promoter"
     gene            411..1211
                     /label="URA3"
                     /gene="URA3"
     CDS             411..1214
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             complement(446..922)
                     /label="ORF frame 3"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(1814..1962)
                     /label="lacZ_a"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    1928..1950
                     /label="M13_pUC_fwd_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1943..1959
                     /label="M13_forward20_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1966..1984
                     /label="T7_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2017..2033
                     /label="pBluescriptSK_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             complement(2830..3333)
                     /label="ORF frame 1"
                     /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
                     NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
                     INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
                     PVVYVRM*"
     CDS             2869..3441
                     /label="ORF frame 1"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(3763..3782)
                     /label="T3_promoter"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(3799..3817)
                     /label="M13_reverse_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    complement(3816..3838)
                     /label="M13_pUC_rev_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     rep_origin      complement(4190..4809)
                     /label="pBR322_origin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     gene            complement(4964..5824)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     CDS             complement(4964..5824)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5866..5894)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
  241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
  301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
  361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
  421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
  481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
  541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
  601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
  661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
  721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
  781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
  841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
  901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
  961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
 1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
 1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
 1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
 1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
 1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
 1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
 1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
 1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
 1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
 1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
 1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
 1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
 1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
 1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctaTACC
 2041 CATACGATGT TCCTGACTAT GCGGGCTATC CGTATGACGT CCCGGACTAT GCAGGcTCCT
 2101 ATCCATATGA CGTTCCAGAT TACGCTGCTC AGTGCtgaaG ATcCGAAGTT CCTATACTTT
 2161 CTAGAGAATA GGAACTTCgt gcgcttttga accacgtaaa gtgctgtaaa Ccagctctat
 2221 tcaatgaggt aaatgataaa gtttataaga gaccaaaaaa ttcaaaaaaa aaattgaaaa
 2281 attatattgg tatttatatg aaacaatagt tcagttcaaa gcgctagctt ttctcatctc
 2341 aactaaccgg tattaggtgg gtatttattt aatatgggaa caagaaatgt gacaTtaatg
 2401 ttcatgtttt tattttcatt atttattcta tatggtttgg gacttgatgt tatgtcttaa
 2461 ttcatagaGA TCCgcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
 2521 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
 2581 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
 2641 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
 2701 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
 2761 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
 2821 gttctcacat cacatccgaa cataaacaac cgGATCgatc ctacaattAT GTCTACTACT
 2881 TTGGATGATA CTGCTTATAG ATACAGAACT TCTGTTCCAG GTGATGCTGA AGCTATTGAA
 2941 GCTTTGGATG GTTCTTTCAC TACCGATACT GTTTTTAGAG TTACTGCTAC TGGTGATGGT
 3001 TTCACTTTGA GAGAAGTTCC AGTTGATCCA CCATTGACTA AGGTTTTCCC AGATGATGAA
 3061 TCCGATGATG AATCCGATGC TGGTGAAGAT GGTGATCCAG ATTCTAGAAC TTTCGTTGCT
 3121 TATGGTGATG ATGGTGATTT GGCTGGTTTC GTTGTTGTTT CTTATTCTGG TTGGAACAGA
 3181 AGATTGACTG TTGAAGATAT TGAAGTTGCT CCAGAACATA GAGGTCATGG TGTTGGTAGA
 3241 GCTTTGATGG GTTTGGCTAC TGAATTCGCC AGAGAAAGAG GTGCTGGTCA TTTGTGGTTG
 3301 GAAGTTACCA ATGTTAATGC TCCAGCTATT CATGCTTATA GAAGAATGGG TTTCACTTTG
 3361 TGTGGTTTGG ATACTGCTTT ATACGATGGT ACTGCTTCCG ATGGTGAACA AGCTTTGTAT
 3421 ATGTCTATGC CATGTCCATA AtaacTCGAC cagtactgac aataaaaaga ttcttgtttt
 3481 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
 3541 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
 3601 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
 3661 tcgatactaa cgccgccatc cagtgTCGAt cgacGAAGTT CCTATACTTT CTAGAGAATA
 3721 GGAACTTCGT Cctcgagggg gggcccggta ccagcttttg ttccctttag tgagggttaa
 3781 ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
 3841 caattccaca caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag
 3901 tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
 3961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
 4021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
 4081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
 4141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcC GCGTTGCTGG
 4201 CGTTTTTCCA TAGGCTCGGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
 4261 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT CCCCCCTGGA AGCTCCCTCG
 4321 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
 4381 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
 4441 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
 4501 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
 4561 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
 4621 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
 4681 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
 4741 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
 4801 CTTTGATCTt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
 4861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
 4921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagTTACCAA TGCTTAATCA
 4981 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTGCCCG
 5041 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
 5101 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
 5161 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
 5221 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
 5281 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
 5341 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGAAAAAA AGCGGTTAGC TCCTTCGGTC
 5401 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
 5461 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
 5521 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
 5581 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
 5641 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
 5701 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
 5761 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
 5821 TCATactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
 5881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
 5941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
 6001 ggcgtatcac gaggcccttt cgtc
//

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