出品人: | Irene Castano Navarro(2015) |
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载体名称: | pYC48 |
质粒类型: | 酿酒酵母表达载体;Recipient integrative Vector;FLP/FRT系统载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | -- |
载体大小: | 6024 bp |
5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
3' 测序引物及序列: | -- |
载体标签: | HA |
载体抗性: | 氨苄青霉素 |
筛选标记: | URA3,Nourseothricin |
克隆菌株: | DH10B感受态细胞 ,30°C |
宿主细胞(系): | 出芽酵母(酿酒酵母)、白色念珠菌 |
备注: | -- |
产品目录号: | #63905 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pYC48
价格:1200元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYC48 6024 bp DNA SYN DEFINITION pYC48 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6024 /organism="pYC48" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" promoter 196..409 /label="URA3_promoter" gene 411..1211 /label="URA3" /gene="URA3" CDS 411..1214 /label="ORF frame 3" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*" CDS complement(446..922) /label="ORF frame 3" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature complement(1814..1962) /label="lacZ_a" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature 1928..1950 /label="M13_pUC_fwd_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" promoter 1943..1959 /label="M13_forward20_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" promoter 1966..1984 /label="T7_promoter" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature 2017..2033 /label="pBluescriptSK_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" CDS complement(2830..3333) /label="ORF frame 1" /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID PVVYVRM*" CDS 2869..3441 /label="ORF frame 1" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(3763..3782) /label="T3_promoter" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(3799..3817) /label="M13_reverse_primer" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" misc_feature complement(3816..3838) /label="M13_pUC_rev_primer" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" rep_origin complement(4190..4809) /label="pBR322_origin" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" gene complement(4964..5824) /label="Ampicillin" /gene="Ampicillin" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" CDS complement(4964..5824) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5866..5894) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG 421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA 481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT 541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA 601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT 661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC 721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG 781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC 841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT 901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG 961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG 1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG 1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG 1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG 1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA 1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC 1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA 1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG 1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG 1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg 1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta 1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctaTACC 2041 CATACGATGT TCCTGACTAT GCGGGCTATC CGTATGACGT CCCGGACTAT GCAGGcTCCT 2101 ATCCATATGA CGTTCCAGAT TACGCTGCTC AGTGCtgaaG ATcCGAAGTT CCTATACTTT 2161 CTAGAGAATA GGAACTTCgt gcgcttttga accacgtaaa gtgctgtaaa Ccagctctat 2221 tcaatgaggt aaatgataaa gtttataaga gaccaaaaaa ttcaaaaaaa aaattgaaaa 2281 attatattgg tatttatatg aaacaatagt tcagttcaaa gcgctagctt ttctcatctc 2341 aactaaccgg tattaggtgg gtatttattt aatatgggaa caagaaatgt gacaTtaatg 2401 ttcatgtttt tattttcatt atttattcta tatggtttgg gacttgatgt tatgtcttaa 2461 ttcatagaGA TCCgcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc 2521 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc 2581 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc 2641 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc 2701 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt 2761 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca 2821 gttctcacat cacatccgaa cataaacaac cgGATCgatc ctacaattAT GTCTACTACT 2881 TTGGATGATA CTGCTTATAG ATACAGAACT TCTGTTCCAG GTGATGCTGA AGCTATTGAA 2941 GCTTTGGATG GTTCTTTCAC TACCGATACT GTTTTTAGAG TTACTGCTAC TGGTGATGGT 3001 TTCACTTTGA GAGAAGTTCC AGTTGATCCA CCATTGACTA AGGTTTTCCC AGATGATGAA 3061 TCCGATGATG AATCCGATGC TGGTGAAGAT GGTGATCCAG ATTCTAGAAC TTTCGTTGCT 3121 TATGGTGATG ATGGTGATTT GGCTGGTTTC GTTGTTGTTT CTTATTCTGG TTGGAACAGA 3181 AGATTGACTG TTGAAGATAT TGAAGTTGCT CCAGAACATA GAGGTCATGG TGTTGGTAGA 3241 GCTTTGATGG GTTTGGCTAC TGAATTCGCC AGAGAAAGAG GTGCTGGTCA TTTGTGGTTG 3301 GAAGTTACCA ATGTTAATGC TCCAGCTATT CATGCTTATA GAAGAATGGG TTTCACTTTG 3361 TGTGGTTTGG ATACTGCTTT ATACGATGGT ACTGCTTCCG ATGGTGAACA AGCTTTGTAT 3421 ATGTCTATGC CATGTCCATA AtaacTCGAC cagtactgac aataaaaaga ttcttgtttt 3481 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt 3541 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc 3601 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat 3661 tcgatactaa cgccgccatc cagtgTCGAt cgacGAAGTT CCTATACTTT CTAGAGAATA 3721 GGAACTTCGT Cctcgagggg gggcccggta ccagcttttg ttccctttag tgagggttaa 3781 ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 3841 caattccaca caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag 3901 tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 3961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 4021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 4081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 4141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcC GCGTTGCTGG 4201 CGTTTTTCCA TAGGCTCGGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 4261 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT CCCCCCTGGA AGCTCCCTCG 4321 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 4381 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 4441 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 4501 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 4561 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 4621 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 4681 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 4741 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 4801 CTTTGATCTt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 4921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagTTACCAA TGCTTAATCA 4981 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTGCCCG 5041 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 5101 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 5161 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 5221 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 5281 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 5341 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGAAAAAA AGCGGTTAGC TCCTTCGGTC 5401 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 5461 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 5521 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 5581 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 5641 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 5701 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 5761 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 5821 TCATactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 5881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 5941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 6001 ggcgtatcac gaggcccttt cgtc //