| 质粒名称: | pLZ349 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000619 |
| 产品用途: | Mammalian Cre-dependent VanR-specific 8xANR-NSP3 expression vector (VanO5-PhCMVmin-loxP-STOP-loxP-(ANR)8-NSP3-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | VanO5-PhCMVmin |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: | (ANR)8-NSP3 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ349
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ349载体质粒基本信息
pLZ349质粒图谱载体图谱和pLZ349载体序列质粒序列多克隆位点信息
pLZ349质粒载体简介
pLZ349质粒序列载体序列
LOCUS pLZ349 7693 bp DNA circular SYN 14-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7693)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7693
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 228
/label=E2F-1
misc_feature 232
/label=CMV
misc_feature 234..245
/label=VanO
/label=nonstandard type: operator
misc_feature 248..259
/label=VanO
/label=nonstandard type: operator
misc_feature 262..273
/label=VanO
/label=nonstandard type: operator
misc_feature 276..287
/label=VanO
/label=nonstandard type: operator
misc_feature 290..301
/label=VanO
/label=nonstandard type: operator
misc_feature 302..306
/label=E2F-1
misc_feature 307..455
/label=hcmvmin
misc_feature 377..391
/label=New Feature
primer_bind 436..456
/label=181-WKG-593-F
misc_feature 478..485
/label=New Feature
misc_feature 488..492
/label=NheI
misc_recomb 497..530
/label=LoxP
protein_bind complement(497..530)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
polyA_signal 586..810
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
terminator 819..1003
/label=SV40 late polyA
misc_feature 1040..1073
/label=loxP
protein_bind complement(1040..1073)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
polyA_signal 1080..1128
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
misc_feature 1141
/label=New Feature
misc_feature 1141
/label=New Feature
regulatory 1147..1156
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1155
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 1156..1218
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1219..1293
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1294..1356
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1357..1431
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1438..1500
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1501..1575
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1576..1638
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1639..1713
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1720..1782
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1783..1857
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1858..1920
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1921..1995
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2002..2064
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2065..2139
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2140..2202
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2203..2277
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 2284..3225
/label=NSP3
misc_feature 2284..2307
/label=NSP3
primer_bind complement(2295..2314)
/label=179-WKG-592-R
misc_feature 3253..3258
/label=New Feature
misc_feature 3253..3258
/label=New Feature
polyA_signal 3290..3514
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3560..3988
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
primer_bind complement(3574..3598)
/label=184-WKG-599-R
promoter 4002..4331
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
misc_feature 4234..4283
/label=P_SV40 TSS-region
misc_feature 4398..5189
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(4442..4469)
/label=188-WKG-610-R
polyA_signal 5366..5487
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5532..5552)
/label=M13-rev
primer_bind complement(5536..5552)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5558..5580)
/label=LacO
protein_bind 5560..5576
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5584..5614)
/label=lac promoter
protein_bind 5629..5650
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5756..6696
/label=ColE1
/note="ColE1"
misc_feature complement(6700..7557)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(7558..7662)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
301 tctagctcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
421 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc tcgcccgggg
481 atcctcgcta gcgcttataa cttcgtatag catacattat acgaagttat cggcgcgcca
541 atgctccgaa tgcatcagtg cccctgcagg gctgatcagc ctcgactgtg ccttctagtt
601 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
661 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
721 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
781 ggcatgctgg ggatgcggtg ggctctatgg cttctgagga taagatacat tgatgagttt
841 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
901 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
961 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcgc ttggtaccgt
1021 ctttccagtg gttaattaaa taacttcgta tagcatacat tatacgaagt tatcgatccg
1081 aacaaacgac ccaacacccg tgcgttttat tctgtctttt tattgccgat cctcgagctt
1141 aagcttgcca ccatggggga gctcgacgag ttggtttatc tcttagacgg acccggatat
1201 gaccccattc attcagatgg ctccggaacg ggcagcggga ccggaagtgg caccggaacc
1261 acttccggaa ccggtactgg cgggagtacc ggcggtgagc tagacgaact cgtgtacctg
1321 ttggatggcc ccggttacga cccaatccac agcgatggaa gtggaactgg gtctgggact
1381 gggtccggca ccgggactac ttcaggcacc ggcactgggg gctccactgg ccataccggg
1441 gagctcgacg agttggttta tctcttagac ggacccggat atgaccccat tcattcagat
1501 ggctccggaa cgggcagcgg gaccggaagt ggcaccggaa ccacttccgg aaccggtact
1561 ggcgggagta ccggcggtga gctagacgaa ctcgtgtacc tgttggatgg ccccggttac
1621 gacccaatcc acagcgatgg aagtggaact gggtctggga ctgggtccgg caccgggact
1681 acttcaggca ccggcactgg gggctccact ggccataccg gggagctcga cgagttggtt
1741 tatctcttag acggacccgg atatgacccc attcattcag atggctccgg aacgggcagc
1801 gggaccggaa gtggcaccgg aaccacttcc ggaaccggta ctggcgggag taccggcggt
1861 gagctagacg aactcgtgta cctgttggat ggccccggtt acgacccaat ccacagcgat
1921 ggaagtggaa ctgggtctgg gactgggtcc ggcaccggga ctacttcagg caccggcact
1981 gggggctcca ctggccatac cggggagctc gacgagttgg tttatctctt agacggaccc
2041 ggatatgacc ccattcattc agatggctcc ggaacgggca gcgggaccgg aagtggcacc
2101 ggaaccactt ccggaaccgg tactggcggg agtaccggcg gtgagctaga cgaactcgtg
2161 tacctgttgg atggccccgg ttacgaccca atccacagcg atggaagtgg aactgggtct
2221 gggactgggt ccggcaccgg gactacttca ggcaccggca ctgggggctc cactggccat
2281 atgatgctca agatggagtc tacacagcag atggctagtt cgattattaa cacttctttt
2341 gaagccgcag tcgttgctgc cacttcaact cttgaactta tgggcattca atatgattat
2401 aatgaagttt atactagagt taagagcaaa tttgattacg taatggatga ttcaggtgtt
2461 aaaaacaatc tgttgggtaa agcggcaact attgatcagg cactgaatgg aaaatttggt
2521 tcagcagcaa gaaatagaaa ttggatggct gatactagaa ctacagctag actagatgaa
2581 gatgtgaaca aacttagaat gatgctatca tctaaaggaa ttgatcaaaa aatgagagta
2641 cttaatgcat gtttcaatgt gaagcgagtt ccaggtaaat catcatcaat aattaaatgt
2701 actagattaa tgagagataa gattgaacgt ggtgaagtcg aagtggatga ttcgtttgtt
2761 gaagaaaaaa tggaagttga cacaattgat tggaaatcta gatatgaaca attggaaaaa
2821 cgatttgaat cgctgaaaca aagggtgaat gaaaaatata caagttgggt gcaaaaagca
2881 aaaaaggtaa atgaaaatat gtactctctc caaaacgtta tatcacaaca gcagagccag
2941 attgctgatt tacaaaatta ctgtaataaa ttggaagttg atctacaaaa taaaattagt
3001 tcacttgtgt cttcagtcga atggtacctg aaatcaatgg aattgcctga tgaaattaag
3061 acagatattg aacaacaact aaattccatt gatgtaatca atccaattaa tgctatagat
3121 gattttgaat cactgatcag aaatattata ctcgactatg acagaatatt tttaatgttt
3181 aaaggattaa tgaggcaatg caactatgaa tatacgtatg aataggatcc actagtccag
3241 tgtggtgaag cttctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
3301 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
3361 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
3421 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
3481 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
3541 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
3601 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
3661 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
3721 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
3781 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
3841 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
3901 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
3961 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
4021 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
4081 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
4141 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
4201 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
4261 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
4321 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
4381 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
4441 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
4501 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
4561 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
4621 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
4681 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
4741 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
4801 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
4861 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
4921 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
4981 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
5041 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
5101 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
5161 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
5221 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
5281 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
5341 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
5401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
5461 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
5521 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
5581 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
5641 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
5701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
5761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
5821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
5881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
5941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
6001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
6061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
6121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
6181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
6241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
6301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
6361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
6421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
6481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
6541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
6601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
6661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
6721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
6781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
6841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
6901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
6961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
7021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
7081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
7141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
7201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
7261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
7321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
7381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
7441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
7501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
7561 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
7621 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
7681 gccacctgac gtc
//
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