TOPFlash

价格:1350元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Millipore
载体名称: TOPFlash
质粒类型: 信号通路报告系统载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: --
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: 21-170
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

TOPFlash载体图谱



TOPFlash载体特征

载体简介

 The Wnt/Wg family encodes secreted glycoproteins implicated in cell growth regulation, cell fate
determination, organogenesis and oncogenesis. Inferred from genetic analysis of wingless (wg) signaling in
Drosophila, in mammalian cells, Wnts signal through the Frizzled family of receptors, to Dishevelled. Dishevelled
down regulates glycogen synthase kinase-3  (GSK-3 ) activity. This results in the stabilization and accumulation of
cytosolic  -catenin, a GSK-3  substrate, which when phosphorylated by GSK-3, is targeted for ubiquitin-mediated
proteolysis. -catenin then translocates to the nucleus, where in complexes with members of the Tcf/LEF family of
transcription regulators, activates transcription of Tcf-responsive genes. This Tcf-reporter plasmid, along with
FOPFLASH (Cat. # 21-169) enables quantitation of Wnt/Wg signaling in cells transfected with these constructs.

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