LOCUS rtTA-N144 9792 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION 2nd generation lentiviral transfer plasmid. Expresses the reverse
tetracycline-controlled transactivator (rtTA) with hygromycin
resistance.
ACCESSION .
VERSION .
KEYWORDS rtTA-N144
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9792)
AUTHORS Richner M, Victor MB, Liu Y, Abernathy D, Yoo AS
TITLE MicroRNA-based conversion of human fibroblasts into striatal medium
spiny neurons.
JOURNAL Nat Protoc. 2015 Oct;10(10):1543-55. doi: 10.1038/nprot.2015.102.
Epub 2015 Sep 17.
PUBMED 26379228
REFERENCE 2 (bases 1 to 9792)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9792
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2221..2432
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
intron 2433..3371
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3328..3348
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3390..4136
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
promoter 4185..4684
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4220..4243)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4617..4636
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 4711..5736
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
misc_feature 5760..6348
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5813..5833)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6231..6242)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6555..7188
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7316..7332)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7316..7332)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7329..7351)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7340..7356
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7364..7394)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7409..7430
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7547..7564)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7718..8306)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7798..7817)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8477..9337)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9100..9119
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9338..9442)
/gene="bla"
/label=AmpR promoter
polyA_signal 9490..9624
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9527..9546)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9581..9600
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
3361 ttccatttca ggtgtcgtga ggatccacca tgtctagact ggacaagagc aaagtcataa
3421 acggcgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg
3481 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
3541 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
3601 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc
3661 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
3721 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
3781 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
3841 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
3901 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
3961 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg
4021 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
4081 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaaggat
4141 ccgcggccgc gccggctcta gatcgcgaac gcgtgaattc taccgggtag gggaggcgct
4201 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
4261 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
4321 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
4381 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
4441 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
4501 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
4561 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
4621 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
4681 ttcgacctgc agcccaagct tcctaggacc atgaaaaagc ctgaactcac cgcgacgtct
4741 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
4801 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
4861 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
4921 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
4981 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
5041 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
5101 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
5161 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
5221 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
5281 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
5341 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
5401 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
5461 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
5521 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
5581 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
5641 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
5701 cgacgcccca gcactcgtcc gagggcaaag gaatagcccg ggcggggcgc gtctggaaca
5761 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
5821 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
5881 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
5941 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
6001 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
6061 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
6121 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
6181 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
6241 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
6301 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
6361 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
6421 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
6481 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
6541 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
6601 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
6661 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
6721 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
6781 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
6841 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
6901 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
6961 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
7021 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
7081 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
7141 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
7201 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
7261 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
7321 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
7381 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
7441 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
7501 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
7561 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
7621 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
7681 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
7741 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
7801 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
7861 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
7921 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
7981 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
8041 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
8101 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
8161 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
8221 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
8281 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
8341 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
8401 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
8461 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
8521 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
8581 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
8641 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
8701 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
8761 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
8821 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
8881 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
8941 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
9001 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
9061 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
9121 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
9181 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
9241 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
9301 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
9361 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
9421 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
9481 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
9541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
9601 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
9661 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
9721 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
9781 aacttagtag tt
//
