pMMB207

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: pMMB207
质粒类型: 细菌表达载体;广宿主载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Tac
载体大小: 9075 bp
5' 测序引物及序列 Tac promoter:GAGCGGATAACAATTTCACACAGG
3' 测序引物及序列: pBAD Reverse: GATTTAATCTGTATCAGG
载体标签: --
载体抗性: 氨苄青霉素氯霉素(10 mg/ml Chloramphenicol)
表达菌株: 原核细胞
备注: --
产品目录号: 37809
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pMMB207载体图谱



pMMB207 多克隆位点

载体简介

载体序列

LOCUS       pMMB207	9075 bp 	DNA	SYN
DEFINITION  pMMB207
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="pMMB207"
                     /mol_type="other DNA"
     misc_feature    complement(8..25)
                     /label="pBAD_rev_primer"
     misc_feature    complement(8..25)
                     /label="pTrcHis_rev_primer"
     terminator      58..215
                     /label="rrnB_terminator"
     terminator      181..224
                     /label="rrnB_T1_terminator"
     terminator      356..383
                     /label="rrnB_T2_terminator"
     promoter        424..452
                     /label="AmpR_promoter"
     gene            494..673
                     /label="Ampicillin (0 - 179)"
                     /gene="Ampicillin (0 - 179)"
     gene            1047..1706
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             1047..1706
                     /label="ORF frame 3"
     CDS             complement(2813..3469)
                     /label="ORF frame 3"
     CDS             2942..5071
                     /label="ORF frame 2"
     CDS             complement(4217..4810)
                     /label="ORF frame 3"
     CDS             4527..5171
                     /label="ORF frame 3"
     CDS             complement(5579..6346)
                     /label="ORF frame 3"
     CDS             5582..6421
                     /label="ORF frame 2"
     CDS             6309..7259
                     /label="ORF frame 3"
     promoter        7420..7450
                     /label="P5_promoter"
     misc_feature    complement(7536..8626)
                     /label="lacI"
     CDS             complement(7679..8494)
                     /label="ORF frame 3"
     promoter        8926..8954
                     /label="tac_promoter"
     misc_feature    8966..8988
                     /label="M13_pUC_rev_primer"
ORIGIN
    1 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
   61 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
  121 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
  181 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
  241 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
  301 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
  361 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
  421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
  481 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
  541 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
  601 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
  661 gagttttcgc cccgaagaac gtgatgtccg gcggtgcttt tgccgttacg caccaccccg
  721 tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc aagcacctca
  781 aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg
  841 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
  901 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
  961 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
 1021 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
 1081 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
 1141 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
 1201 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
 1261 aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
 1321 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
 1381 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
 1441 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
 1501 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
 1561 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
 1621 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
 1681 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattgtctt caaattcccg
 1741 ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
 1801 atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
 1861 agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
 1921 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
 1981 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta actgtcacga
 2041 acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct gcaataactg
 2101 tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt tggaaaaatc
 2161 catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct tgtggggcgc
 2221 tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt cgctgcgcta
 2281 ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc
 2341 ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc ggcctttccc
 2401 cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc
 2461 agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt
 2521 caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg ctgttcacct
 2581 tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg cgctcttgct
 2641 gctgttccct tgcccgctcc cgctgaattt cggcattgat tcgcgctcgt tgttcttcga
 2701 gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca ccccattgtt
 2761 aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga ccctactttg
 2821 taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc cacccttcgg
 2881 gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac agcgaggcag
 2941 catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc aatcggccag
 3001 ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt
 3061 gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact actgggatgc
 3121 tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc
 3181 ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc agcacctgac
 3241 cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg agaacccgca
 3301 ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg ccgctcagtg
 3361 gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga ccgaagcgct
 3421 caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg ccaaccgggc
 3481 attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg cgcagggcat
 3541 cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag gccggggcat
 3601 ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc agatcatcga
 3661 cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg aagaaatcca
 3721 gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg gcgacactgg
 3781 ccgacgaagc ccggcaggtc atgagccaga ccaagcaggc cagcgaggcg caggcggcgg
 3841 agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc aaagagttgc
 3901 gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt
 3961 ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg gtggtgctgc
 4021 tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac ggctcgatct
 4081 ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct
 4141 caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgcaccca ccggccagat
 4201 gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat
 4261 gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt
 4321 gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc
 4381 tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc
 4441 cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga
 4501 cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga
 4561 caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa
 4621 gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca
 4681 gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag
 4741 ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg
 4801 cttcggtcat gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg
 4861 ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg
 4921 caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc
 4981 cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat
 5041 ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata
 5101 ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc
 5161 ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc
 5221 aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg
 5281 ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga
 5341 ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg
 5401 tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct
 5461 ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc
 5521 agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc
 5581 aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct
 5641 ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg
 5701 tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct
 5761 gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc
 5821 caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca
 5881 agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc
 5941 cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga
 6001 cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat
 6061 cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc
 6121 cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc
 6181 ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc
 6241 cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa
 6301 ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct
 6361 caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta
 6421 agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc
 6481 tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact
 6541 acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc
 6601 gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac
 6661 ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca
 6721 ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag
 6781 aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg
 6841 tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg
 6901 ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg
 6961 tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg
 7021 aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca
 7081 aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca
 7141 tgcgcaagcg ccgcaagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga
 7201 cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac
 7261 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
 7321 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
 7381 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
 7441 gatgtatgct cttctgctcc cgaacgccag caagacgtag cccagcgcgt cggccagctt
 7501 gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
 7561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
 7621 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
 7681 accgcctggc cctgagagag ttgcagcaag cggtccacgt ggtttgcccc agcaggcgaa
 7741 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
 7801 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
 7861 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
 7921 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
 7981 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
 8041 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
 8101 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
 8161 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
 8221 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
 8281 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
 8341 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
 8401 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
 8461 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
 8521 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
 8581 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
 8641 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
 8701 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
 8761 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
 8821 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
 8881 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
 8941 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg
 9001 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg
 9061 gcggatgaga gaaga
//

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