LOCUS pcDNA3.0-Magneto2.0-p2A-mCherry 9796 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bicistronic expression of Magneto2.0 and mCherry in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.0-Magneto2.0-p2A-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9796)
AUTHORS Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM,
Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann
CD, Guler AD
TITLE Genetically targeted magnetic control of the nervous system.
JOURNAL Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
PUBMED 26950006
REFERENCE 2 (bases 1 to 9796)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9796
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 916..925
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 922..4545
/codon_start=1
/product="fusion between a C-terminally truncated rat TRPV4
cation channel, the human ferritin light chain, the human
ferritin heavy chain, and a plasma membrane targeting
signal from the Kir2.1 potassium channel (Wheeler et al.,
2016)"
/label=Magneto2.0
/note="can be used as a magnetically sensitive actuator of
neuronal activity"
/translation="MADPGDGPRAAPGDVAEPPGDESGTSGGEAFPLSSLANLFEGEEG
SSSLSPVDASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPM
DSLFDYGTYRHHPSDNKRWRRKVVEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGST
ADLDGLLSYLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGN
MREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGY
FYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTK
FVTKMYDLLLLKCSRLFPDSNLETVLNNDGLSPLMMAAKTGKIGVFQHIIRREVTDEDT
RHLSRKFKDWAYGPVYSSLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINEL
LRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVIT
LLTGVLFFFTSIKDLFMKKCPGVNSLFVDGSFQLLYFIYSVLVVVSAALYLAGIEAYLA
VMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVTL
LNPCTNMKVCNEDQSNCTVPSYPACRDSETFSAFLLDLFKLTIGMGDLEMLSSAKYPVV
FILLLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLR
KAFRSGARGGGGSDYKDDDDKGGGGSSRVMGSQIRQNYSTDVEAAVNSLVNLYLQASYT
YLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDE
WGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDH
LTNLHRLGGPEAGLGEYLFERLTLKHDARGGGGSDYKDDDDKGGGGSSRVMTTASTSQV
RQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAE
KLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKND
PHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNESARG
GGGSDYKDDDDKGGGGSSRVASKSRITSEGEYIPLDQIDINV"
CDS 4561..4617
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4624..5331
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(4766..4784)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 5034..5053
/label=mCherry-F
/note="mCherry, forward primer"
promoter complement(5349..5367)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5350..5367)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5387..5404)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5393..5617
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5663..6091
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5750..5769)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5960..5981
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6100..6120)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6105..6434
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6285..6420
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6347..6366
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6501..7295
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6555..6574)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7165..7184
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7469..7590
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7506..7525)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7560..7579
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7639..7655)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7639..7655)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7652..7674)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7663..7679
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7687..7717)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7732..7753
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7870..7887)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8041..8629)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8121..8140)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8800..9660)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9423..9442
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9661..9765)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gggtctagag gtaccgccac catggcagat cctggtgatg gcccccgtgc agcgcctggg
961 gatgtggctg agccccctgg agacgagagt ggcacttctg gtggggaggc cttccccctc
1021 tcttccctgg ccaacctgtt tgagggagag gaaggctcct cttctctttc accagtggat
1081 gctagccgcc ctgctggccc cggggatgga cgtccaaacc tgcgtatgaa gttccagggc
1141 gctttccgca agggggttcc caaccccatt gacctgctgg agtccaccct gtatgagtcc
1201 tcagtagtgc ctgggcccaa gaaagcgccc atggattcgt tgttcgacta tggcacttac
1261 cggcaccacc ccagtgacaa caagagatgg aggaggaagg tcgtagagaa gcagccacag
1321 agccccaaag ctcccgcccc ccagccaccc cccatcctca aagtcttcaa ccggcccatc
1381 ctctttgaca tcgtgtcccg gggctccact gccgacctgg acggactgct ctcctacttg
1441 ctgacccaca agaagcgcct gactgatgag gagttccggg aaccatccac agggaagacc
1501 tgcctgccca aggcacttct gaacttaagc aatggccgaa acgacaccat cccagtgttg
1561 ctggacattg cggaacgcac gggcaacatg cgggagttca tcaactcgcc cttcagagac
1621 atctactacc gagggcagac ggcactgcac atcgccattg aacggcgctg caagcattac
1681 gtggagctcc tggtggccca gggagccgat gtgcacgcgc aggcccgagg gcggttcttc
1741 cagcccaagg atgagggtgg ctacttctac tttggggagc tgcccttgtc cttggcagcc
1801 tgcaccaacc agccgcacat cgtcaactac ctgacagaga accctcacaa gaaagccgat
1861 atgaggcgac aggactccag aggcaacacg gtgctccacg cgctggtggc catcgctgac
1921 aacacccgag agaacaccaa gtttgtcacc aagatgtatg acctgttgct tctcaagtgc
1981 tcccgcctct tcccagacag caacctggag actgtgctta acaatgacgg tctttcgccc
2041 ctcatgatgg ctgccaagac tggcaagatc ggggtctttc agcacatcat ccgacgggag
2101 gtgacagatg aggacacacg gcacctgtct cgcaagttca aggactgggc ctacgggcct
2161 gtgtattctt ctctctacga cctctcctcc ctggatacgt gcggggagga agtgtccgtg
2221 ctggagatcc tggtttacaa cagcaagatc gagaaccgcc atgagatgct ggctgtggag
2281 cccattaacg aactgctgag ggacaagtgg cgtaagttcg gggccgtgtc cttctacatc
2341 aacgttgtct cctatctgtg tgccatggtc atcttcaccc tcacagccta ctatcagcca
2401 ctggagggca cgccacccta cccttaccgt accacggtgg actacctgag gctggctggt
2461 gaggtcatca cgctcctcac aggagtcctg ttcttcttta ccagtatcaa agacttgttc
2521 atgaagaaat gccctggagt gaattctctc ttcgtcgatg gctccttcca gttgctctac
2581 ttcatctact cagtgctggt ggttgtgtct gcggcgctct acctggcagg gatcgaggcc
2641 tatctggctg tgatggtctt tgccctggtc ctgggctgga tgaatgccct ttacttcacc
2701 cgtgggctga agctgacagg gacctacagc atcatgattc agaagatcct cttcaaagat
2761 ctcttccgct ttctgctggt ctacctgctt tttatgattg gctatgcctc agctctggtc
2821 accctcctga atccgtgcac caacatgaag gtctgtaacg aggaccagag caactgcacg
2881 gtgccctcat accccgcgtg ccgggacagc gagaccttca gcgccttcct actggacctc
2941 ttcaagctca ccatcggcat gggcgacctg gagatgctga gcagcgctaa gtaccccgtg
3001 gtcttcattc tcctgctggt tacctacatc atcctcacct tcgtgctcct gctgaacatg
3061 ctcatcgccc tcatgggtga gaccgtgggc caggtgtcca aggagagcaa gcacatctgg
3121 aagctgcagt gggccaccac catcctggac atcgagcgct ccttccctgt gttcctgagg
3181 aaggccttcc gctccggagc acggggcggg ggcggttctg attataagga cgacgacgac
3241 aaaggaggag gcggctcctc tcgggttatg ggctcccaga ttcgtcagaa ttattccacc
3301 gacgtggagg cagccgtcaa cagcctggtc aatttgtacc tgcaggcctc ctacacctac
3361 ctctctctgg gcttctattt cgaccgcgat gatgtggctc tggaaggcgt gagccacttc
3421 ttccgcgaat tggccgagga gaagcgcgag ggctacgagc gtctcctgaa gatgcaaaac
3481 cagcgtggcg gccgcgctct cttccaggac atcaagaagc cagctgaaga tgagtggggt
3541 aaaaccccag acgccatgaa agctgccatg gccctggaga aaaagctgaa ccaggccctt
3601 ttggatcttc atgccctggg ttctgcccgc acggaccccc atctctgtga cttcctggag
3661 actcacttcc tagatgagga agtgaagctt atcaagaaga tgggtgacca cctgaccaac
3721 ctccacaggc tgggtggccc ggaggctggg ctgggcgagt atctcttcga aaggctcact
3781 ctcaagcacg acgcccgcgg cggcggcggc tccgattata aagatgatga tgataaaggc
3841 ggcggcggct cctcccgcgt catgacgacc gcgtccacct cgcaggtgcg ccagaactac
3901 caccaggact cagaggccgc catcaaccgc cagatcaacc tggagctcta cgcctcctac
3961 gtttacctgt ccatgtctta ctactttgac cgcgatgatg tggctttgaa gaactttgcc
4021 aaatactttc ttcaccaatc tcatgaggag agggaacatg ctgagaaact gatgaagctg
4081 cagaaccaac gaggtggccg aatcttcctt caggatatca agaaaccaga ctgtgatgac
4141 tgggagagcg ggctgaatgc aatggagtgt gcattacatt tggaaaaaaa tgtgaatcag
4201 tcactactgg aactgcacaa actggccact gacaaaaatg acccccattt gtgtgacttc
4261 attgagacac attacctgaa tgagcaggtg aaagccatca aagaattggg tgaccacgtg
4321 accaacttgc gcaagatggg agcgcccgaa tctggcttgg cggaatatct ctttgacaag
4381 cacaccctgg gagacagtga taatgaaagc gcacgcgggg gtggcgggag cgactataaa
4441 gacgacgatg acaaaggggg tggaggctcc tcccgagtgg ctagcaagag cagaatcacc
4501 agcgagggcg agtacatccc cctggaccag atcgacatca acgtgggatc aggcagcggc
4561 gccacgaact tctctctgtt aaagcaagca ggagacgtgg aagaaaaccc cggtcccgga
4621 tccgtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
4681 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
4741 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
4801 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
4861 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
4921 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
4981 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
5041 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
5101 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
5161 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
5221 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
5281 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta aggcgcgccg
5341 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
5401 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
5461 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
5521 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
5581 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
5641 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
5701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
5761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
5821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
5881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
5941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
6001 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
6061 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
6121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
6181 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
6241 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
6301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
6361 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
6421 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
6481 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
6541 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
6601 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
6661 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
6721 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
6781 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
6841 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
6901 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
6961 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
7021 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
7081 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
7141 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
7201 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
7261 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
7321 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
7381 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
7441 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
7501 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
7561 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
7621 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
7681 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
7741 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
7801 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
8221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
8281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
8341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
8401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
8461 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
8521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
8581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
8641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
8701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
8761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
8821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
8881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
8941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
9001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
9061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
9121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
9181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
9241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
9301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
9361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
9421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
9481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
9541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
9601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
9661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
9721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
9781 agtgccacct gacgtc
//
